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The map label for this gene is tilS [H]

Identifier: 99082202

GI number: 99082202

Start: 2484016

End: 2485308

Strand: Reverse

Name: tilS [H]

Synonym: TM1040_2362

Alternate gene names: 99082202

Gene position: 2485308-2484016 (Counterclockwise)

Preceding gene: 99082203

Following gene: 99082201

Centisome position: 77.64

GC content: 63.34

Gene sequence:

>1293_bases
GTGCTCCTGACACCGACAGAAGAGGCGCTGACCTCTGCGATCCGCGCCCGTATCGGTCCCTTGCCCGAAAAACTTGGCGT
CGCTGTCTCCGGAGGCAGCGATTCCGTTGCGTTGATGCATGTGCTTGCGACCATGGCTCGTGCAGAAGACACAGCGCTGG
CGGTGGCCACGGTGGATCACGGGTTGCGCCCCGAATCACGGCGTGAAGCCGAGTTTGTGGCGCAGCAAGCACATGACCTT
GGGCTCTCGCATCAGACCCTGACATGGCAACATCCCGCTGACAGCGGAAACCTTCAGGCAGAGGCCCGCGCTGCGCGCTA
TCGCTTGCTGGGAGACTGGGCACGGACGCAGGGCCTCGGCGCGGTGGCGGTCGGGCATACCGCGGACGATCAGGCTGAAA
CCGTTCTGATGCGGTTGCGACGGGCCTCTGGGGTGGCCGGGCTCTGCGGTATGCCGACATGTCACCACCGCGACGGGATC
GATGTCCTGCGTCCGATGCTGGATCTGCGCCGTAGCGATCTGCGCGCTTATCTTACGGCGCGCGATGTGGCTTGGATCGA
GGATCCCTCAAACCTTGATATGAAGTTCGAGCGTGTGCGTATACGCGATGCGCTCAACGCGCTGGAGCCTTTGGGTCTGA
CCGTGGACGCGCTGACAGCGGTGGCTGACAATATGCGCATGGCCAATAGGGCATTGGACAGTGCGACACGCACCGCTGCC
GCGTCGATCGGGCAGATCCGTCTTGGCATGGTGGTCCTTGATTGTGCAGGGTTTAATCGGCTTGAGACCGAAATCGCGCG
TCGTCTTCTGGTGTCGGCACTTCTGCACACAAACGGCGCCGCATACCCGCCGCGCCGGGCGCCTGTTCTCGATGCATTGA
AGGCGCTGAAAGAGGGGCGGGGCGCAACCTTGCAGGGCTGCCATATCCTCGTGCGGGGCGGTGCGATCTGGGTTGGGCGC
GAATATGCCTCCGTGCAACACTCCGCATGCGCCCTCTTGCCCGGTGAGGGTGGTGTCGCAACGATGGATGCAACTTGGGA
CGCGGCATGGCACATCAAGGCGCCTGCCGGATGGAGCCGACGCGTCGAGATTCGCGCGCTCGGAGCCGAAGGCCTTGCCG
AAATCCCCGATTGGCGCAGTCACGACGCCCCGCGCGCCCTTTTGAAATCTCTTCCGGGGGTCTGGTGTGATGGCCAGTTG
ATCGCCGCGCCGCTGCTTCAGGAGCGCTCTGGCTGGACGATTGCGGCAATTCGACAGCCTGAGACATGGGCACTGTCTGC
TTTATCGCATTGA

Upstream 100 bases:

>100_bases
GGCAGCTCGATCAGGCTTGTATCACGCTGGCCGAGGTCGCCGTGCGTTTCCCCAATGATCCCGCCGTGACCGCAGCCGAA
GCGGAGCGGTCCAAACTGGC

Downstream 100 bases:

>100_bases
ACCTCACCAAAACCTCCCTATTTTAGGTGCAACTTATGGTCGTCGCGTGCTAGGGCCGTGACGTCCTTGGAATTCTTGGG
AGAGCCATTTTGGGCAACGC

Product: tRNA(Ile)-lysidine synthetase-like

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MLLTPTEEALTSAIRARIGPLPEKLGVAVSGGSDSVALMHVLATMARAEDTALAVATVDHGLRPESRREAEFVAQQAHDL
GLSHQTLTWQHPADSGNLQAEARAARYRLLGDWARTQGLGAVAVGHTADDQAETVLMRLRRASGVAGLCGMPTCHHRDGI
DVLRPMLDLRRSDLRAYLTARDVAWIEDPSNLDMKFERVRIRDALNALEPLGLTVDALTAVADNMRMANRALDSATRTAA
ASIGQIRLGMVVLDCAGFNRLETEIARRLLVSALLHTNGAAYPPRRAPVLDALKALKEGRGATLQGCHILVRGGAIWVGR
EYASVQHSACALLPGEGGVATMDATWDAAWHIKAPAGWSRRVEIRALGAEGLAEIPDWRSHDAPRALLKSLPGVWCDGQL
IAAPLLQERSGWTIAAIRQPETWALSALSH

Sequences:

>Translated_430_residues
MLLTPTEEALTSAIRARIGPLPEKLGVAVSGGSDSVALMHVLATMARAEDTALAVATVDHGLRPESRREAEFVAQQAHDL
GLSHQTLTWQHPADSGNLQAEARAARYRLLGDWARTQGLGAVAVGHTADDQAETVLMRLRRASGVAGLCGMPTCHHRDGI
DVLRPMLDLRRSDLRAYLTARDVAWIEDPSNLDMKFERVRIRDALNALEPLGLTVDALTAVADNMRMANRALDSATRTAA
ASIGQIRLGMVVLDCAGFNRLETEIARRLLVSALLHTNGAAYPPRRAPVLDALKALKEGRGATLQGCHILVRGGAIWVGR
EYASVQHSACALLPGEGGVATMDATWDAAWHIKAPAGWSRRVEIRALGAEGLAEIPDWRSHDAPRALLKSLPGVWCDGQL
IAAPLLQERSGWTIAAIRQPETWALSALSH
>Mature_430_residues
MLLTPTEEALTSAIRARIGPLPEKLGVAVSGGSDSVALMHVLATMARAEDTALAVATVDHGLRPESRREAEFVAQQAHDL
GLSHQTLTWQHPADSGNLQAEARAARYRLLGDWARTQGLGAVAVGHTADDQAETVLMRLRRASGVAGLCGMPTCHHRDGI
DVLRPMLDLRRSDLRAYLTARDVAWIEDPSNLDMKFERVRIRDALNALEPLGLTVDALTAVADNMRMANRALDSATRTAA
ASIGQIRLGMVVLDCAGFNRLETEIARRLLVSALLHTNGAAYPPRRAPVLDALKALKEGRGATLQGCHILVRGGAIWVGR
EYASVQHSACALLPGEGGVATMDATWDAAWHIKAPAGWSRRVEIRALGAEGLAEIPDWRSHDAPRALLKSLPGVWCDGQL
IAAPLLQERSGWTIAAIRQPETWALSALSH

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=217, Percent_Identity=34.5622119815668, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 46298; Mature: 46298

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTPTEEALTSAIRARIGPLPEKLGVAVSGGSDSVALMHVLATMARAEDTALAVATVDH
CCCCCCHHHHHHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCC
GLRPESRREAEFVAQQAHDLGLSHQTLTWQHPADSGNLQAEARAARYRLLGDWARTQGLG
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
AVAVGHTADDQAETVLMRLRRASGVAGLCGMPTCHHRDGIDVLRPMLDLRRSDLRAYLTA
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDVAWIEDPSNLDMKFERVRIRDALNALEPLGLTVDALTAVADNMRMANRALDSATRTAA
HCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIGQIRLGMVVLDCAGFNRLETEIARRLLVSALLHTNGAAYPPRRAPVLDALKALKEGR
HHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
GATLQGCHILVRGGAIWVGREYASVQHSACALLPGEGGVATMDATWDAAWHIKAPAGWSR
CCEEECCEEEEECCEEEEECHHHHHCCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCC
RVEIRALGAEGLAEIPDWRSHDAPRALLKSLPGVWCDGQLIAAPLLQERSGWTIAAIRQP
EEEEEEECCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEECC
ETWALSALSH
CHHHHHCCCC
>Mature Secondary Structure
MLLTPTEEALTSAIRARIGPLPEKLGVAVSGGSDSVALMHVLATMARAEDTALAVATVDH
CCCCCCHHHHHHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCC
GLRPESRREAEFVAQQAHDLGLSHQTLTWQHPADSGNLQAEARAARYRLLGDWARTQGLG
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
AVAVGHTADDQAETVLMRLRRASGVAGLCGMPTCHHRDGIDVLRPMLDLRRSDLRAYLTA
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDVAWIEDPSNLDMKFERVRIRDALNALEPLGLTVDALTAVADNMRMANRALDSATRTAA
HCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIGQIRLGMVVLDCAGFNRLETEIARRLLVSALLHTNGAAYPPRRAPVLDALKALKEGR
HHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
GATLQGCHILVRGGAIWVGREYASVQHSACALLPGEGGVATMDATWDAAWHIKAPAGWSR
CCEEECCEEEEECCEEEEECHHHHHCCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCC
RVEIRALGAEGLAEIPDWRSHDAPRALLKSLPGVWCDGQLIAAPLLQERSGWTIAAIRQP
EEEEEEECCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEECC
ETWALSALSH
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA