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The map label for this gene is sigW [H]

Identifier: 99082017

GI number: 99082017

Start: 2288888

End: 2289514

Strand: Reverse

Name: sigW [H]

Synonym: TM1040_2177

Alternate gene names: 99082017

Gene position: 2289514-2288888 (Counterclockwise)

Preceding gene: 99082018

Following gene: 99082016

Centisome position: 71.53

GC content: 65.55

Gene sequence:

>627_bases
ATGACCGCCGAGACGGGCGTTGATCGTGCGCATGGCGCGACGCGGGCCACCTTTGTGGAGCCCGCGCTCAGCGACGAAGC
CTTGCTGATCCGCTTCGGCAATGGCGATCCTTCGGCGGCGACAGAGCTGACCCGGAGGCTTGCGCCGCGGGTGCTGTCAC
TGGCGACACGGGTATTGGGCAATCGCGCAGAGGCCGAAGACGTGACCCAGGAAGCGATGCTCAGGCTGTGGAAAATCGCG
CCCGACTGGCGAAATGGCGAGGCCCGCGTCTCTACTTGGCTCTATCGTGTGGGGATGAATCTTTGCATCGACCACAAACG
CCGGGTTCGTGGCGGGCATGTGGATCTGGACGCGATCCCGGAACCTCCTGACCCTGCGGCCGGGGCGGCGGAGGCTCTTC
AGGAGGGGGCGCGCCGGGACGCGCTGCAAGATGCGCTGATGACCTTGCCCGAGCGGCAACGTCAGGCCGTGGTGCTGCGC
CACATAGAGGAACTGACGAACCCGGAAATCGCCGGGGTCATGGAGATCAGCGTGGAGGCCGTGGAAAGCCTGACCGCACG
GGGCAAACGCGCTTTGGCCGCAGCATTGGCGGGTCGGCGCGAGGAATTGGGGTATCAAGATGGGTAA

Upstream 100 bases:

>100_bases
CGTGCAGGTGCGGGCCAGGACTCGGAGTAAACCTGATCCAAACGACACAGTGCATATGGGTGCCAAAAAACCGGTGTCTC
CGCTTGCGAGCGGAGCACCG

Downstream 100 bases:

>100_bases
CAAGCTAAATAGCCAGACCGAAATTGACGATCTCGACGCACTCTTTGCCGAGGCGCGCGCGACCCGTCCCGAGGTGCCCG
CCTCCCTGATGGAGTCCATC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTAETGVDRAHGATRATFVEPALSDEALLIRFGNGDPSAATELTRRLAPRVLSLATRVLGNRAEAEDVTQEAMLRLWKIA
PDWRNGEARVSTWLYRVGMNLCIDHKRRVRGGHVDLDAIPEPPDPAAGAAEALQEGARRDALQDALMTLPERQRQAVVLR
HIEELTNPEIAGVMEISVEAVESLTARGKRALAAALAGRREELGYQDG

Sequences:

>Translated_208_residues
MTAETGVDRAHGATRATFVEPALSDEALLIRFGNGDPSAATELTRRLAPRVLSLATRVLGNRAEAEDVTQEAMLRLWKIA
PDWRNGEARVSTWLYRVGMNLCIDHKRRVRGGHVDLDAIPEPPDPAAGAAEALQEGARRDALQDALMTLPERQRQAVVLR
HIEELTNPEIAGVMEISVEAVESLTARGKRALAAALAGRREELGYQDG
>Mature_207_residues
TAETGVDRAHGATRATFVEPALSDEALLIRFGNGDPSAATELTRRLAPRVLSLATRVLGNRAEAEDVTQEAMLRLWKIAP
DWRNGEARVSTWLYRVGMNLCIDHKRRVRGGHVDLDAIPEPPDPAAGAAEALQEGARRDALQDALMTLPERQRQAVVLRH
IEELTNPEIAGVMEISVEAVESLTARGKRALAAALAGRREELGYQDG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=180, Percent_Identity=29.4444444444444, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22689; Mature: 22558

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAETGVDRAHGATRATFVEPALSDEALLIRFGNGDPSAATELTRRLAPRVLSLATRVLG
CCCCCCCCHHCCCCHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
NRAEAEDVTQEAMLRLWKIAPDWRNGEARVSTWLYRVGMNLCIDHKRRVRGGHVDLDAIP
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EPPDPAAGAAEALQEGARRDALQDALMTLPERQRQAVVLRHIEELTNPEIAGVMEISVEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VESLTARGKRALAAALAGRREELGYQDG
HHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAETGVDRAHGATRATFVEPALSDEALLIRFGNGDPSAATELTRRLAPRVLSLATRVLG
CCCCCCCHHCCCCHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
NRAEAEDVTQEAMLRLWKIAPDWRNGEARVSTWLYRVGMNLCIDHKRRVRGGHVDLDAIP
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EPPDPAAGAAEALQEGARRDALQDALMTLPERQRQAVVLRHIEELTNPEIAGVMEISVEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VESLTARGKRALAAALAGRREELGYQDG
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]