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The map label for this gene is yoaE [H]

Identifier: 99082008

GI number: 99082008

Start: 2279428

End: 2281509

Strand: Reverse

Name: yoaE [H]

Synonym: TM1040_2168

Alternate gene names: 99082008

Gene position: 2281509-2279428 (Counterclockwise)

Preceding gene: 99082009

Following gene: 99082007

Centisome position: 71.28

GC content: 62.2

Gene sequence:

>2082_bases
ATGTCCAGACAAGCCAGATCCTCTCAAGACACGCGTGTCGTGCCCAGCGTTTGCCCGTTGGACTGCCCGGACACCTGTAG
CCTCAGCGTCGAGGTAACGGGCGATCAGATTACTGCCGTACGCGGGTCAAATGCCAATCCGTTTACGGCCGGTGTGGTCT
GCAACAAGGTCACGCGCGCCTACCCTGACTTTGTACATGGTCCGGCGCGGCTCACACATCCGCTGCGGCGGGTGGGGCCG
CGGGGGGCTGGCACGTTTGAACGCATCAGTTGGGAGGATGCGCTGGATCTTGTGGTCGAAGGGTTTCACGCTGCCATTGA
TGCGCATGGCGCGCAGTCGATCTTGCCGCTGAACTATGCTGGACCTCACGGGGAGCTGGCGGGGGGCTCGCTCGATCGGC
GATTTTTCTACCGTCTGGGCGCCACGCGAATGGATCGCGGCCCGCTCTGCGGCGGCGTGCGCAGCCTCGCCTATGAGAGC
CTCTATGGCAATGCTCCCGGCATGCCGCCGGAACAGGCCCAGCATGCCGATCTCATCGTGGTCTGGGGCAACAATGTGAC
GGTCTCCAACCTGCATCTGACACGGATCGTCAAAGCGGCCCGCGCCAAGGGTGCGCGTCTGGTGGTGATTGACCCCAAGC
GGATCAAGATCGCAGAGCAGGCCGACCTCTATCTTCAGGTGCGACCCGGAACCGATGTGGTGCTTGCCCATGCGCTTGCG
GCTGAACTCGAACGGCGCGGCGCATTTGATGCGGAGTTTATTGCGCATTGGGTGCATGGCGTTGACGAGTATATGGACGA
GGCGCGCAAGCACAGCGTTGAAAACGCTTCTGAAATCTGCGGCCTCGCTGTCAGCGATATCCTCAAGCTGGCGGATTGGA
TGCAGACTGCGCAGAGGCTGGCCACCTCCACCGGCAATGGGATGGAACGGGGACGCTCCGGAGGTTCGGGGCTGCGCGCG
GCGATGGCGCTCAATGCGCTCCTGGGACAGCACGGGCGACTGGGAGCCGGGGTGGTGGCCAAATCCGGACTGGCCTCCCC
CAAGACACGCGCCCGGCTGCAGGGCGATCATCTGATCGACCCTGAAACACGGGTGGTGAATATCCTTGATGTGGCACAGC
TGATGCTTGATCGCGAGCAATCCACACCGATCAATGCCGTCATGATCTACAACCACAATCCTGTCGCGACACACCCCGAC
CAGACTCGGATGATTGCGGCGTTGAGTCAGCCTGAGATCTTTACCGTTGGCTGCGACGTGGTGATGACCGACAGCATGGC
ATATTGCGATGTGATCCTGCCCGCAGCCACCCACTTTGAGCACGATGAGATCTTTGCCGCCTATGGTCAGAACTATGTCC
AGCGGGCCGAGCCCGTGATCGCGCCCATCGCGGAAAGTCTGCCGAACACGGAGATTTTCCGCCGTCTGGCCGCGCGTTTT
GGCTATATCGAGCCAGAGCATCAGGCTTCGGACGTGCAGTTGATGGATGACGCGATGGATGCCAGCCATCCGCAATTTGA
AGGCCGCCCCCCAAGCCAGATGCCGGTGGATCGCGCGTTGGAAATGCAAAAAAGCGATGGCGCTGCTCTGGTCCTCTGCG
AGACGGTGACGCCCGACACAGAAACAGGCCGGATTGAGCTGTTCAGCGCCGCGCTCGAGGCGGAGACCGGCTTTGGCGTG
CCGCGCTATGATCCGGTGTCGCAGGACCTGCCTTTGGTGTTGATTACCCCGAGTTCGGACAAACGCATCAACGCCACTTT
CGGCAGCGCGCCGCTTTCCGCAGGGCCCGAGAGCATCGAGGTGCACCCCGAAGACGCCGCCGCACGGGGGCTGCAGGATG
GTGATTTGGTCGAGGTTGCCAACGCCCGCGGAAGCGTCACGTTCCGTCTCAAGATCTCGGATGCGATGCAGCGCGGTGTG
ACGTATGCCCCCAAGGGTACATGGCGCATCAGTTCCAAGACGGGCCTCACCGCAAACGCTCTGATCCCGGTGGAGGTACG
CACCGATATTGGCGACGGGGCCTGCTATAACGAGACCTTCGTTGAGCTGCGCGCCTATGCCGGGGTGCGCGCCGCCGAGT
GA

Upstream 100 bases:

>100_bases
TCGAAGTCTAAAATTGGACTGCTCTTTTTATCGGATATTGGCGCTTTTGACAGGGCTTCTTTTGCACTAGCCGGAGAGGA
CGCCAAAAGTGGAGCCTTCG

Downstream 100 bases:

>100_bases
GTGCGCATCTGGGAAAGACAGGGGCCGGGCGTTGTGCCCGGTCTTTGCTTGTCTGTCGATTGGCGCAGGCATAACGTTGC
CAAGAGGACACTCTGATCGG

Product: molydopterin dinucleotide-binding region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MSRQARSSQDTRVVPSVCPLDCPDTCSLSVEVTGDQITAVRGSNANPFTAGVVCNKVTRAYPDFVHGPARLTHPLRRVGP
RGAGTFERISWEDALDLVVEGFHAAIDAHGAQSILPLNYAGPHGELAGGSLDRRFFYRLGATRMDRGPLCGGVRSLAYES
LYGNAPGMPPEQAQHADLIVVWGNNVTVSNLHLTRIVKAARAKGARLVVIDPKRIKIAEQADLYLQVRPGTDVVLAHALA
AELERRGAFDAEFIAHWVHGVDEYMDEARKHSVENASEICGLAVSDILKLADWMQTAQRLATSTGNGMERGRSGGSGLRA
AMALNALLGQHGRLGAGVVAKSGLASPKTRARLQGDHLIDPETRVVNILDVAQLMLDREQSTPINAVMIYNHNPVATHPD
QTRMIAALSQPEIFTVGCDVVMTDSMAYCDVILPAATHFEHDEIFAAYGQNYVQRAEPVIAPIAESLPNTEIFRRLAARF
GYIEPEHQASDVQLMDDAMDASHPQFEGRPPSQMPVDRALEMQKSDGAALVLCETVTPDTETGRIELFSAALEAETGFGV
PRYDPVSQDLPLVLITPSSDKRINATFGSAPLSAGPESIEVHPEDAAARGLQDGDLVEVANARGSVTFRLKISDAMQRGV
TYAPKGTWRISSKTGLTANALIPVEVRTDIGDGACYNETFVELRAYAGVRAAE

Sequences:

>Translated_693_residues
MSRQARSSQDTRVVPSVCPLDCPDTCSLSVEVTGDQITAVRGSNANPFTAGVVCNKVTRAYPDFVHGPARLTHPLRRVGP
RGAGTFERISWEDALDLVVEGFHAAIDAHGAQSILPLNYAGPHGELAGGSLDRRFFYRLGATRMDRGPLCGGVRSLAYES
LYGNAPGMPPEQAQHADLIVVWGNNVTVSNLHLTRIVKAARAKGARLVVIDPKRIKIAEQADLYLQVRPGTDVVLAHALA
AELERRGAFDAEFIAHWVHGVDEYMDEARKHSVENASEICGLAVSDILKLADWMQTAQRLATSTGNGMERGRSGGSGLRA
AMALNALLGQHGRLGAGVVAKSGLASPKTRARLQGDHLIDPETRVVNILDVAQLMLDREQSTPINAVMIYNHNPVATHPD
QTRMIAALSQPEIFTVGCDVVMTDSMAYCDVILPAATHFEHDEIFAAYGQNYVQRAEPVIAPIAESLPNTEIFRRLAARF
GYIEPEHQASDVQLMDDAMDASHPQFEGRPPSQMPVDRALEMQKSDGAALVLCETVTPDTETGRIELFSAALEAETGFGV
PRYDPVSQDLPLVLITPSSDKRINATFGSAPLSAGPESIEVHPEDAAARGLQDGDLVEVANARGSVTFRLKISDAMQRGV
TYAPKGTWRISSKTGLTANALIPVEVRTDIGDGACYNETFVELRAYAGVRAAE
>Mature_692_residues
SRQARSSQDTRVVPSVCPLDCPDTCSLSVEVTGDQITAVRGSNANPFTAGVVCNKVTRAYPDFVHGPARLTHPLRRVGPR
GAGTFERISWEDALDLVVEGFHAAIDAHGAQSILPLNYAGPHGELAGGSLDRRFFYRLGATRMDRGPLCGGVRSLAYESL
YGNAPGMPPEQAQHADLIVVWGNNVTVSNLHLTRIVKAARAKGARLVVIDPKRIKIAEQADLYLQVRPGTDVVLAHALAA
ELERRGAFDAEFIAHWVHGVDEYMDEARKHSVENASEICGLAVSDILKLADWMQTAQRLATSTGNGMERGRSGGSGLRAA
MALNALLGQHGRLGAGVVAKSGLASPKTRARLQGDHLIDPETRVVNILDVAQLMLDREQSTPINAVMIYNHNPVATHPDQ
TRMIAALSQPEIFTVGCDVVMTDSMAYCDVILPAATHFEHDEIFAAYGQNYVQRAEPVIAPIAESLPNTEIFRRLAARFG
YIEPEHQASDVQLMDDAMDASHPQFEGRPPSQMPVDRALEMQKSDGAALVLCETVTPDTETGRIELFSAALEAETGFGVP
RYDPVSQDLPLVLITPSSDKRINATFGSAPLSAGPESIEVHPEDAAARGLQDGDLVEVANARGSVTFRLKISDAMQRGVT
YAPKGTWRISSKTGLTANALIPVEVRTDIGDGACYNETFVELRAYAGVRAAE

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds (By Similarity). [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=770, Percent_Identity=24.9350649350649, Blast_Score=165, Evalue=1e-41,
Organism=Escherichia coli, GI171474008, Length=734, Percent_Identity=25.2043596730245, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI87081797, Length=735, Percent_Identity=25.4421768707483, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI3868721, Length=697, Percent_Identity=23.8163558106169, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI145693196, Length=762, Percent_Identity=24.0157480314961, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI87081994, Length=766, Percent_Identity=23.1070496083551, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1787231, Length=777, Percent_Identity=23.9382239382239, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 74727; Mature: 74596

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQARSSQDTRVVPSVCPLDCPDTCSLSVEVTGDQITAVRGSNANPFTAGVVCNKVTRA
CCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEHHHHHHHHHHH
YPDFVHGPARLTHPLRRVGPRGAGTFERISWEDALDLVVEGFHAAIDAHGAQSILPLNYA
CHHHHCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
GPHGELAGGSLDRRFFYRLGATRMDRGPLCGGVRSLAYESLYGNAPGMPPEQAQHADLIV
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VWGNNVTVSNLHLTRIVKAARAKGARLVVIDPKRIKIAEQADLYLQVRPGTDVVLAHALA
EECCCEEEECHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHH
AELERRGAFDAEFIAHWVHGVDEYMDEARKHSVENASEICGLAVSDILKLADWMQTAQRL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATSTGNGMERGRSGGSGLRAAMALNALLGQHGRLGAGVVAKSGLASPKTRARLQGDHLID
HHCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCCC
PETRVVNILDVAQLMLDREQSTPINAVMIYNHNPVATHPDQTRMIAALSQPEIFTVGCDV
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHEEEECCCCCEEEEECCE
VMTDSMAYCDVILPAATHFEHDEIFAAYGQNYVQRAEPVIAPIAESLPNTEIFRRLAARF
EEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
GYIEPEHQASDVQLMDDAMDASHPQFEGRPPSQMPVDRALEMQKSDGAALVLCETVTPDT
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCC
ETGRIELFSAALEAETGFGVPRYDPVSQDLPLVLITPSSDKRINATFGSAPLSAGPESIE
CCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEE
VHPEDAAARGLQDGDLVEVANARGSVTFRLKISDAMQRGVTYAPKGTWRISSKTGLTANA
ECCCHHHHCCCCCCCEEEEECCCCCEEEEEEEHHHHHCCCEECCCCCEEECCCCCCEECC
LIPVEVRTDIGDGACYNETFVELRAYAGVRAAE
EEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRQARSSQDTRVVPSVCPLDCPDTCSLSVEVTGDQITAVRGSNANPFTAGVVCNKVTRA
CCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEHHHHHHHHHHH
YPDFVHGPARLTHPLRRVGPRGAGTFERISWEDALDLVVEGFHAAIDAHGAQSILPLNYA
CHHHHCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
GPHGELAGGSLDRRFFYRLGATRMDRGPLCGGVRSLAYESLYGNAPGMPPEQAQHADLIV
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VWGNNVTVSNLHLTRIVKAARAKGARLVVIDPKRIKIAEQADLYLQVRPGTDVVLAHALA
EECCCEEEECHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHH
AELERRGAFDAEFIAHWVHGVDEYMDEARKHSVENASEICGLAVSDILKLADWMQTAQRL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATSTGNGMERGRSGGSGLRAAMALNALLGQHGRLGAGVVAKSGLASPKTRARLQGDHLID
HHCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCCC
PETRVVNILDVAQLMLDREQSTPINAVMIYNHNPVATHPDQTRMIAALSQPEIFTVGCDV
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHEEEECCCCCEEEEECCE
VMTDSMAYCDVILPAATHFEHDEIFAAYGQNYVQRAEPVIAPIAESLPNTEIFRRLAARF
EEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
GYIEPEHQASDVQLMDDAMDASHPQFEGRPPSQMPVDRALEMQKSDGAALVLCETVTPDT
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCC
ETGRIELFSAALEAETGFGVPRYDPVSQDLPLVLITPSSDKRINATFGSAPLSAGPESIE
CCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEE
VHPEDAAARGLQDGDLVEVANARGSVTFRLKISDAMQRGVTYAPKGTWRISSKTGLTANA
ECCCHHHHCCCCCCCEEEEECCCCCEEEEEEEHHHHHCCCEECCCCCEEECCCCCCEECC
LIPVEVRTDIGDGACYNETFVELRAYAGVRAAE
EEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]