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The map label for this gene is gdhA [C]

Identifier: 99081990

GI number: 99081990

Start: 2257115

End: 2258545

Strand: Reverse

Name: gdhA [C]

Synonym: TM1040_2150

Alternate gene names: 99081990

Gene position: 2258545-2257115 (Counterclockwise)

Preceding gene: 99081994

Following gene: 99081987

Centisome position: 70.56

GC content: 61.15

Gene sequence:

>1431_bases
ATGAGCAATCTCAAAGAACCAAGCTTCCGTGAAAGTGTGGATCTCATGTTCAACCGCGCCGTGGCGCTGATGGATTTGCC
GCCGGGGCTTGAGGAAAAGATCCGCGTCTGTAACGCCACCTACACGGTCCGCTTTGGGGTGCGCCTGCGCGGGCAGATCC
ACACGTTCACCGGGTATCGCTCGGTCCACTCGGAGCATATGGAGCCGGTCAAAGGCGGCATTCGTTACGCGATGGGGGTG
AACCAGGACGAGGTGGAGGCGCTCGCGGCGCTGATGACCTACAAATGCGCGCTGGTGGAAGCGCCCTTTGGGGGCTCCAA
GGGCGGTCTGCGCATCGACCCGCGTCAGTATGACGAACACGAGCTAGAGCTGATCACCCGTCGTTTTGCCTATGAGCTGG
CCAAACGCGACCTGATCAACCCCTCGCAGAACGTGCCCGCGCCCGACATGGGCACCGGCGAACGCGAGATGGCCTGGATG
GCGGACCAATACGCCCGGATGAACACCACGGACATCAACGCCCGCGCCTGTGTCACCGGCAAGCCGCTCAATGCTGGCGG
TATTGCAGGCCGCGTCGAGGCCACGGGCCGGGGCGTGCAGTACGCACTGCAGGAGTTCTTCCGGTACGAGGAAGACAAGG
CCAAGGCCGGTCTCTCTGGCACCCTCGATGGCAAGCGCGTCATCGTGCAGGGCCTCGGGAATGTGGGCTATCACGCGGCC
AAGTTCCTCTCCGAGGAAGATGGCTGCAAGATCGTTGCGGTGATTGAACGCGATGGCGGTCTCTACGATGAGAACGGGCT
CGACATCGAAGACCTGCGCGCGTGGATCGAAAAGCATGACGGCGTCGATGGCTACCCGTCGGGACGCTACATCAAGAACG
GCGCCAAATACCTCGAAGAGGACTGCGACATCCTGATCCCGGCGGCGCTTGAGGGGGTGATCAACCTCTCCAACGCCGAA
AACGTGAAGGCCAAGCTGATCATCGAGGCTGCGAACGGTCCTGTGACCGCGGGCGCAGATGAGATCCTGCGCAAGAAGGG
CACGGTGATCATTCCGGACATGTATGCCAACGCCGGCGGTGTGACGGTTTCCTACTTTGAGTGGGTCAAGAACCTCTCGC
ATATCCGCTTTGGCCGGATGCAGCGCCGTCAGGAAGAGGCACGCCACCAGCTTGTGGTCGATCAACTGGAAATGCTCTCT
GAGAGCATGGGCAAGACATGGTCGCTCGATCCCAAGTTCAAGGAAAAATACCTCCGCGGTGCAGGTGAGCTGGAGCTGGT
GCGCTCGGGTCTCGATGACACCATGCGGATTGCCTATCAGTCCATGCGGCAGGTGTGGCACGAACGCGATGACGTCTCTG
ATCTGCGCACCGCCGCATATCTCGTCTCCATCGACAAGGTTGCCTCGAGCTACCGCGCCAAGGGCCTCTGA

Upstream 100 bases:

>100_bases
GTGCAAAATCGGGGAATATCAGTCGCAGTTGGTAACGTTCTCTGCATAGATGGGACGCATAGTAACCACTTGAGTCGTTT
CAGAAGGACGGGATCACCCC

Downstream 100 bases:

>100_bases
GCCCTATTCATATCTTTGGAAAGCGGCGGGTGTTATCCCGCCGTTTTTCTATGGGCGACGATCACGCGACAGGCCCGCTG
CGCCGTCTTGTGACCCGAAA

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSNLKEPSFRESVDLMFNRAVALMDLPPGLEEKIRVCNATYTVRFGVRLRGQIHTFTGYRSVHSEHMEPVKGGIRYAMGV
NQDEVEALAALMTYKCALVEAPFGGSKGGLRIDPRQYDEHELELITRRFAYELAKRDLINPSQNVPAPDMGTGEREMAWM
ADQYARMNTTDINARACVTGKPLNAGGIAGRVEATGRGVQYALQEFFRYEEDKAKAGLSGTLDGKRVIVQGLGNVGYHAA
KFLSEEDGCKIVAVIERDGGLYDENGLDIEDLRAWIEKHDGVDGYPSGRYIKNGAKYLEEDCDILIPAALEGVINLSNAE
NVKAKLIIEAANGPVTAGADEILRKKGTVIIPDMYANAGGVTVSYFEWVKNLSHIRFGRMQRRQEEARHQLVVDQLEMLS
ESMGKTWSLDPKFKEKYLRGAGELELVRSGLDDTMRIAYQSMRQVWHERDDVSDLRTAAYLVSIDKVASSYRAKGL

Sequences:

>Translated_476_residues
MSNLKEPSFRESVDLMFNRAVALMDLPPGLEEKIRVCNATYTVRFGVRLRGQIHTFTGYRSVHSEHMEPVKGGIRYAMGV
NQDEVEALAALMTYKCALVEAPFGGSKGGLRIDPRQYDEHELELITRRFAYELAKRDLINPSQNVPAPDMGTGEREMAWM
ADQYARMNTTDINARACVTGKPLNAGGIAGRVEATGRGVQYALQEFFRYEEDKAKAGLSGTLDGKRVIVQGLGNVGYHAA
KFLSEEDGCKIVAVIERDGGLYDENGLDIEDLRAWIEKHDGVDGYPSGRYIKNGAKYLEEDCDILIPAALEGVINLSNAE
NVKAKLIIEAANGPVTAGADEILRKKGTVIIPDMYANAGGVTVSYFEWVKNLSHIRFGRMQRRQEEARHQLVVDQLEMLS
ESMGKTWSLDPKFKEKYLRGAGELELVRSGLDDTMRIAYQSMRQVWHERDDVSDLRTAAYLVSIDKVASSYRAKGL
>Mature_475_residues
SNLKEPSFRESVDLMFNRAVALMDLPPGLEEKIRVCNATYTVRFGVRLRGQIHTFTGYRSVHSEHMEPVKGGIRYAMGVN
QDEVEALAALMTYKCALVEAPFGGSKGGLRIDPRQYDEHELELITRRFAYELAKRDLINPSQNVPAPDMGTGEREMAWMA
DQYARMNTTDINARACVTGKPLNAGGIAGRVEATGRGVQYALQEFFRYEEDKAKAGLSGTLDGKRVIVQGLGNVGYHAAK
FLSEEDGCKIVAVIERDGGLYDENGLDIEDLRAWIEKHDGVDGYPSGRYIKNGAKYLEEDCDILIPAALEGVINLSNAEN
VKAKLIIEAANGPVTAGADEILRKKGTVIIPDMYANAGGVTVSYFEWVKNLSHIRFGRMQRRQEEARHQLVVDQLEMLSE
SMGKTWSLDPKFKEKYLRGAGELELVRSGLDDTMRIAYQSMRQVWHERDDVSDLRTAAYLVSIDKVASSYRAKGL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=459, Percent_Identity=44.880174291939, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI4885281, Length=457, Percent_Identity=45.2954048140044, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1788059, Length=355, Percent_Identity=31.2676056338028, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17544676, Length=494, Percent_Identity=42.3076923076923, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324951, Length=366, Percent_Identity=27.3224043715847, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319256, Length=362, Percent_Identity=27.6243093922652, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45553475, Length=497, Percent_Identity=44.466800804829, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45549226, Length=510, Percent_Identity=43.5294117647059, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24649460, Length=404, Percent_Identity=45.7920792079208, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24649283, Length=502, Percent_Identity=39.8406374501992, Blast_Score=348, Evalue=4e-96,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 53046; Mature: 52915

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLKEPSFRESVDLMFNRAVALMDLPPGLEEKIRVCNATYTVRFGVRLRGQIHTFTGYR
CCCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCEEEEEEEEEEECEEEEEHHHH
SVHSEHMEPVKGGIRYAMGVNQDEVEALAALMTYKCALVEAPFGGSKGGLRIDPRQYDEH
HHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECCCCCCHH
ELELITRRFAYELAKRDLINPSQNVPAPDMGTGEREMAWMADQYARMNTTDINARACVTG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECC
KPLNAGGIAGRVEATGRGVQYALQEFFRYEEDKAKAGLSGTLDGKRVIVQGLGNVGYHAA
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHCCCCHHHHH
KFLSEEDGCKIVAVIERDGGLYDENGLDIEDLRAWIEKHDGVDGYPSGRYIKNGAKYLEE
HHHCCCCCCEEEEEEECCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
DCDILIPAALEGVINLSNAENVKAKLIIEAANGPVTAGADEILRKKGTVIIPDMYANAGG
CCCEEEHHHHHHHEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCC
VTVSYFEWVKNLSHIRFGRMQRRQEEARHQLVVDQLEMLSESMGKTWSLDPKFKEKYLRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHCC
AGELELVRSGLDDTMRIAYQSMRQVWHERDDVSDLRTAAYLVSIDKVASSYRAKGL
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNLKEPSFRESVDLMFNRAVALMDLPPGLEEKIRVCNATYTVRFGVRLRGQIHTFTGYR
CCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCEEEEEEEEEEECEEEEEHHHH
SVHSEHMEPVKGGIRYAMGVNQDEVEALAALMTYKCALVEAPFGGSKGGLRIDPRQYDEH
HHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECCCCCCHH
ELELITRRFAYELAKRDLINPSQNVPAPDMGTGEREMAWMADQYARMNTTDINARACVTG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECC
KPLNAGGIAGRVEATGRGVQYALQEFFRYEEDKAKAGLSGTLDGKRVIVQGLGNVGYHAA
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHCCCCHHHHH
KFLSEEDGCKIVAVIERDGGLYDENGLDIEDLRAWIEKHDGVDGYPSGRYIKNGAKYLEE
HHHCCCCCCEEEEEEECCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
DCDILIPAALEGVINLSNAENVKAKLIIEAANGPVTAGADEILRKKGTVIIPDMYANAGG
CCCEEEHHHHHHHEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCC
VTVSYFEWVKNLSHIRFGRMQRRQEEARHQLVVDQLEMLSESMGKTWSLDPKFKEKYLRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHCC
AGELELVRSGLDDTMRIAYQSMRQVWHERDDVSDLRTAAYLVSIDKVASSYRAKGL
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]