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The map label for this gene is hisF [H]

Identifier: 99081881

GI number: 99081881

Start: 2147963

End: 2148724

Strand: Direct

Name: hisF [H]

Synonym: TM1040_2041

Alternate gene names: 99081881

Gene position: 2147963-2148724 (Clockwise)

Preceding gene: 99081880

Following gene: 99081882

Centisome position: 67.1

GC content: 62.73

Gene sequence:

>762_bases
ATGCTAAAGACCCGTCTCATTCCCTGTCTCGATGTTGCCGATGGCCGTGTGGTCAAAGGCGTGAATTTTGTTGGCCTACG
CGATGCGGGCGACCCGGTAGAAGCCGCGCGCGCCTATGATGCGGCCGGAGCCGACGAGATCTGTTTCCTCGACATCCATG
CAACCCACGAGAACCGTGGCACCATGTTTGACATGGTCCGCCGCACCGCCGAGCAGTGCTTTGTACCGCTCACTGTGGGG
GGCGGCGTACGCACCAAGGACGATGTGCGGGCGCTATTGCTGGCGGGGGCCGACAAGGTCTCGTTCAATTCCGCCGCTGT
GGCCAACCCGGATGTGATCGCCGAGGCCGCCGACCAGTTCGGCAGCCAGTGCATCGTCTGCGCCATCGACGCCAAATCCG
TCGCCCCCGGCAAGTGGGAGATTTTCACCCATGGCGGACGTCGCGAGACCGGCATCGACGCGGTGGAATTCGCCCGCTTG
GTGACCGCCAAGGGCGCGGGCGAGATCCTCTTGACCTCGATGGATCGCGACGGCACCAAATCGGGCTTCAACCTCGAGAT
GACCCGCGCGATTTCCGATGCGGTGAATGTGCCGGTGATTGCTTCGGGCGGTGTCGGCACCCTGGATCACCTCGTGGACG
GTGTGACCAAAGGTGGCGCCAGCGCGGTGCTGGCGGCCTCGATCTTCCACTTTGGCGAATACACGGTGCAAGAAGCCAAG
GAACACATGATCGCCAATGGCATCCCGATGAGGCTCCAATGA

Upstream 100 bases:

>100_bases
CAGCCCGATGGTCTAACAGCCTGACAGAGGATCGCGGATTTCCCGCGTCCCGGCGCTATGCCCTCTTTTCCCGCCGCCGG
TTCCGGTCCATAAGGCCCCT

Downstream 100 bases:

>100_bases
CCTTGCTGCACGACCTCGAACAGACCATTCTGTCCCGCAAGGGCACCGATCCCGACACCAGCTGGACCGCGAAGCTCTTG
TCCAAGGGGCCGGAGAAATG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLKTRLIPCLDVADGRVVKGVNFVGLRDAGDPVEAARAYDAAGADEICFLDIHATHENRGTMFDMVRRTAEQCFVPLTVG
GGVRTKDDVRALLLAGADKVSFNSAAVANPDVIAEAADQFGSQCIVCAIDAKSVAPGKWEIFTHGGRRETGIDAVEFARL
VTAKGAGEILLTSMDRDGTKSGFNLEMTRAISDAVNVPVIASGGVGTLDHLVDGVTKGGASAVLAASIFHFGEYTVQEAK
EHMIANGIPMRLQ

Sequences:

>Translated_253_residues
MLKTRLIPCLDVADGRVVKGVNFVGLRDAGDPVEAARAYDAAGADEICFLDIHATHENRGTMFDMVRRTAEQCFVPLTVG
GGVRTKDDVRALLLAGADKVSFNSAAVANPDVIAEAADQFGSQCIVCAIDAKSVAPGKWEIFTHGGRRETGIDAVEFARL
VTAKGAGEILLTSMDRDGTKSGFNLEMTRAISDAVNVPVIASGGVGTLDHLVDGVTKGGASAVLAASIFHFGEYTVQEAK
EHMIANGIPMRLQ
>Mature_253_residues
MLKTRLIPCLDVADGRVVKGVNFVGLRDAGDPVEAARAYDAAGADEICFLDIHATHENRGTMFDMVRRTAEQCFVPLTVG
GGVRTKDDVRALLLAGADKVSFNSAAVANPDVIAEAADQFGSQCIVCAIDAKSVAPGKWEIFTHGGRRETGIDAVEFARL
VTAKGAGEILLTSMDRDGTKSGFNLEMTRAISDAVNVPVIASGGVGTLDHLVDGVTKGGASAVLAASIFHFGEYTVQEAK
EHMIANGIPMRLQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=39.2996108949416, Blast_Score=187, Evalue=4e-49,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=28.9915966386555, Blast_Score=99, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=32.1656050955414, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26632; Mature: 26632

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTRLIPCLDVADGRVVKGVNFVGLRDAGDPVEAARAYDAAGADEICFLDIHATHENRG
CCCCCCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TMFDMVRRTAEQCFVPLTVGGGVRTKDDVRALLLAGADKVSFNSAAVANPDVIAEAADQF
HHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHC
GSQCIVCAIDAKSVAPGKWEIFTHGGRRETGIDAVEFARLVTAKGAGEILLTSMDRDGTK
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
SGFNLEMTRAISDAVNVPVIASGGVGTLDHLVDGVTKGGASAVLAASIFHFGEYTVQEAK
CCCCEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
EHMIANGIPMRLQ
HHHHHCCCCEEEC
>Mature Secondary Structure
MLKTRLIPCLDVADGRVVKGVNFVGLRDAGDPVEAARAYDAAGADEICFLDIHATHENRG
CCCCCCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TMFDMVRRTAEQCFVPLTVGGGVRTKDDVRALLLAGADKVSFNSAAVANPDVIAEAADQF
HHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHC
GSQCIVCAIDAKSVAPGKWEIFTHGGRRETGIDAVEFARLVTAKGAGEILLTSMDRDGTK
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
SGFNLEMTRAISDAVNVPVIASGGVGTLDHLVDGVTKGGASAVLAASIFHFGEYTVQEAK
CCCCEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
EHMIANGIPMRLQ
HHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA