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The map label for this gene is mraW

Identifier: 99081855

GI number: 99081855

Start: 2118068

End: 2119057

Strand: Direct

Name: mraW

Synonym: TM1040_2015

Alternate gene names: 99081855

Gene position: 2118068-2119057 (Clockwise)

Preceding gene: 99081854

Following gene: 99081856

Centisome position: 66.17

GC content: 64.85

Gene sequence:

>990_bases
ATGACGGACCGCACCGCTGCCCCCTCCGGTCCCCACATTCCTGTCCTGCTGAAGCCGCTCCTTGCAGCGGTGGCGCCGGT
CACGGGCCGCTGGCTCGATGGCACCTTTGGCGCGGGCGGCTATACCAAGGGCCTTCTGGCTGCGGGGGCCGATCAGGTCA
TCGGCGTCGACCGTGATCCGCTGGCGTTTGAGATGGCGCAGCCGTGGGCGGCAGAGTATGGCGACCGGCTGGTGCTGCAG
CAGGGCGTGTTCTCGCGCATGGATGAATACGCGCAGGAACTCGATGGCGTGGTGCTGGACCTTGGGGTGTCGTCGATGCA
GCTGGATCTGGCGGAGCGCGGCTTTTCCTTCATGAAGGAGGGTCCGCTCGATATGCGCATGTCGCAAGATGGCCCCTCAG
CGGCGGATCTGGTGGCCGAGCTCAGCGAAGTGCAACTCGCTGATGTTCTTTACAGTTTTGGTGAAGAGCGCGCCTCGCGC
CGGATCGCCAAGGCCATCGTGAAGGAACGCGCGCAGGAGCCGATCACCACCACATTGCGTCTGGCCGAAATCATCGAGGG
CTGCCTGCCCCGCCCCAAACCCGGCCAGTCTCACCCGGCGACCCGCAGCTTTCAGGCGCTGCGCATCGCGGTGAATGGCG
AGTATGAAGAGCTGATGGGTGGGCTTCTGGCCGCCGAACGCGCGCTCAAACCCGGTGGGCTCCTGGCCGTGGTGACCTTC
CATTCGGTCGAAGACCGTATGGTCAAACGCTTCTTTCAGCACCGCGCAAACAAGACCGGAAATGCCAACCGATATGCGCC
CGCGATGGAAGAGCAGCCCAGCCAGTTTGAGCTTGTGACCCGCAAGGCCGTTGGCCCCGACAAGGACGAGCTGGCGCAAA
ACCCGCGTTCGCGCTCGGCGCTCTTGCGGGTGGGGCGGCGCACCGATGCCGCTCCGGTGGAGATCACCGCCAAGGAACTG
AGCATGCCGCAGTTGAAAGAGACGCGCTGA

Upstream 100 bases:

>100_bases
CGAAGAGGTCGAGATGGCCGAGGCCGAGAAGCTGATGGACGAGCTGCCGGATGACTTCGATCCCCTTGAATTCCTAGACG
GCGCCGGAGGCGCATAAGAT

Downstream 100 bases:

>100_bases
TGAGAACCCTCGCCTATATGATGACGATCCTCGCGGTCTTTGGTCTGGCGTTCTGGGCCTACCGCGAGAATTACGCCACC
CAGCAGGTGCTCAAGGAAAC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTDRTAAPSGPHIPVLLKPLLAAVAPVTGRWLDGTFGAGGYTKGLLAAGADQVIGVDRDPLAFEMAQPWAAEYGDRLVLQ
QGVFSRMDEYAQELDGVVLDLGVSSMQLDLAERGFSFMKEGPLDMRMSQDGPSAADLVAELSEVQLADVLYSFGEERASR
RIAKAIVKERAQEPITTTLRLAEIIEGCLPRPKPGQSHPATRSFQALRIAVNGEYEELMGGLLAAERALKPGGLLAVVTF
HSVEDRMVKRFFQHRANKTGNANRYAPAMEEQPSQFELVTRKAVGPDKDELAQNPRSRSALLRVGRRTDAAPVEITAKEL
SMPQLKETR

Sequences:

>Translated_329_residues
MTDRTAAPSGPHIPVLLKPLLAAVAPVTGRWLDGTFGAGGYTKGLLAAGADQVIGVDRDPLAFEMAQPWAAEYGDRLVLQ
QGVFSRMDEYAQELDGVVLDLGVSSMQLDLAERGFSFMKEGPLDMRMSQDGPSAADLVAELSEVQLADVLYSFGEERASR
RIAKAIVKERAQEPITTTLRLAEIIEGCLPRPKPGQSHPATRSFQALRIAVNGEYEELMGGLLAAERALKPGGLLAVVTF
HSVEDRMVKRFFQHRANKTGNANRYAPAMEEQPSQFELVTRKAVGPDKDELAQNPRSRSALLRVGRRTDAAPVEITAKEL
SMPQLKETR
>Mature_328_residues
TDRTAAPSGPHIPVLLKPLLAAVAPVTGRWLDGTFGAGGYTKGLLAAGADQVIGVDRDPLAFEMAQPWAAEYGDRLVLQQ
GVFSRMDEYAQELDGVVLDLGVSSMQLDLAERGFSFMKEGPLDMRMSQDGPSAADLVAELSEVQLADVLYSFGEERASRR
IAKAIVKERAQEPITTTLRLAEIIEGCLPRPKPGQSHPATRSFQALRIAVNGEYEELMGGLLAAERALKPGGLLAVVTFH
SVEDRMVKRFFQHRANKTGNANRYAPAMEEQPSQFELVTRKAVGPDKDELAQNPRSRSALLRVGRRTDAAPVEITAKELS
MPQLKETR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=331, Percent_Identity=39.8791540785499, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=42.2459893048128, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1786270, Length=295, Percent_Identity=43.3898305084746, Blast_Score=212, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472493, Length=338, Percent_Identity=38.4615384615385, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI28571637, Length=338, Percent_Identity=38.4615384615385, Blast_Score=194, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_SILST (Q1GF19)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614009.1
- ProteinModelPortal:   Q1GF19
- SMR:   Q1GF19
- STRING:   Q1GF19
- GeneID:   4077472
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2015
- NMPDR:   fig|292414.1.peg.390
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RQKVIQK
- PhylomeDB:   Q1GF19
- ProtClustDB:   PRK00050
- BioCyc:   SSP292414:TM1040_2015-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35879; Mature: 35748

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 84-84 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRTAAPSGPHIPVLLKPLLAAVAPVTGRWLDGTFGAGGYTKGLLAAGADQVIGVDRDP
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCC
LAFEMAQPWAAEYGDRLVLQQGVFSRMDEYAQELDGVVLDLGVSSMQLDLAERGFSFMKE
CHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCHHHHHC
GPLDMRMSQDGPSAADLVAELSEVQLADVLYSFGEERASRRIAKAIVKERAQEPITTTLR
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAEIIEGCLPRPKPGQSHPATRSFQALRIAVNGEYEELMGGLLAAERALKPGGLLAVVTF
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEH
HSVEDRMVKRFFQHRANKTGNANRYAPAMEEQPSQFELVTRKAVGPDKDELAQNPRSRSA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCHHHHHCCCCHHHH
LLRVGRRTDAAPVEITAKELSMPQLKETR
HHHHCCCCCCCCEEEEHHHCCCCCHHCCC
>Mature Secondary Structure 
TDRTAAPSGPHIPVLLKPLLAAVAPVTGRWLDGTFGAGGYTKGLLAAGADQVIGVDRDP
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCC
LAFEMAQPWAAEYGDRLVLQQGVFSRMDEYAQELDGVVLDLGVSSMQLDLAERGFSFMKE
CHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCHHHHHC
GPLDMRMSQDGPSAADLVAELSEVQLADVLYSFGEERASRRIAKAIVKERAQEPITTTLR
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAEIIEGCLPRPKPGQSHPATRSFQALRIAVNGEYEELMGGLLAAERALKPGGLLAVVTF
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEH
HSVEDRMVKRFFQHRANKTGNANRYAPAMEEQPSQFELVTRKAVGPDKDELAQNPRSRSA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCHHHHHCCCCHHHH
LLRVGRRTDAAPVEITAKELSMPQLKETR
HHHHCCCCCCCCEEEEHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA