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The map label for this gene is proS

Identifier: 99081693

GI number: 99081693

Start: 1952289

End: 1953644

Strand: Reverse

Name: proS

Synonym: TM1040_1852

Alternate gene names: 99081693

Gene position: 1953644-1952289 (Counterclockwise)

Preceding gene: 99081694

Following gene: 99081692

Centisome position: 61.03

GC content: 62.46

Gene sequence:

>1356_bases
ATGCGCCTGTCTCGCTATTTTCTGCCCGTCCTCAAGGAGACCCCCTCCGAGGCGCAGGTCGTCAGCCACCGTTACATGCT
GCGCGCCGGCATGATCAAACAGTCCGCCGCCGGGATCTATTCCTGGCTGCCTCTGGGCTACCGCGTGCTGAAAAAGATCG
AAGGCATCGTTCACGAAGAGCAGATGCGCGCCGGTCACATCCCGATGCTGATGCCCACCATCCAGTCGGCTGACCTGTGG
CGCGAGTCCGGGCGCTATGACGCCTACGGCGAGGAGATGCTGCGCATCCGCGACCGCCATGACCGCGACATGCTCTTTAC
GCCAACCGCCGAGGAACTCATCACCGACATCTTCCGCGCCAATGTCTCAAGCTACAAAGACCTGCCGCTGACGATGTATC
AGATCCAGTGGAAGTTCCGCGACGAGATCCGCCCGCGCTTTGGCGTGATGCGGGGGCGCGAATTCTACATGAAGGACGGT
TACAACTTCGACCTCACCAAAGAGGACGCGCTGCACGCCTACAACCGCCATCTGGTCACCTACCTGCGCACCTATGAGCG
CATGGGGCTACAGGCGATCCCGATGCGCGCCGATGGTGGCCCGATTGGTGGCGACTACACCCACGAATTCCTCGTGCTGG
CTGAAACCGGCGAATCGGAGGTCTTTTATGACAGCGAGATCACCGATCTGACGTTCGGCGCCCGCGAGATCGACTATGAC
AATGTTGAACAGTGTCAGGCGGTGCTCGAAGAGTTCACCTCACGTTACGCCCGCACCGACGAGACCCACGACGAGGCGCT
GTTCAACGCGGTCCCAGAAGAGCGCCGCCGCGTCGCGCGCGGGATCGAGGTCGGCCAGATCTTCTACTTCGGCACCAAAT
ACTCCGAGGCGCTGGGCGCCACCGTGCAGACCGCCGATGGCCAGAGCGTGCCGGTCCACATGGGCTCGCACGGGATTGGT
GTGTCGCGTCTTCTCGGCGCCATCATCGAGGCCAGCCACGACGACAAGGGCATCATCTGGCCCGAAGGTGTGACCCCCTT
CCACTGCGGCATCGTGAACCTCAAGCAGGGCGACGATGAAGCGGATGCCGCCTGCGAGCAGCTCTATGCGGCGCTCACCG
CCATCGGTCTGGAGCCGCTTTATGATGATCGCAAGGAACGTGCGGGCGGCAAATTCGCCAGCATGGATCTCATTGGCCTG
CCATGGCGCATCACCGTCGGCCCGCGCGGTCTGAAGAACGGCGTCGTCGAAGTGACCAGCCGCCGTACCGGCGAAAGCGA
AGAAATGAGCCCGGAAGACGCGGTAAAGAAAATCGCCGCGATCTACGCCAACCACCCCACGCCGCGCGGCTTCTGA

Upstream 100 bases:

>100_bases
CGCGATGCGGGCTCTGGCTGTTTGGCTTGATCTCAACCCTTGTCCGCACCTGCTGCAGCCACTAAACCGGGCAAAACATC
TTTCACCGCATGGAGCCATT

Downstream 100 bases:

>100_bases
AGCCACCGGCACATACAGAATTGAGGCCCCGCCCGACAGGTCCGTCGGGCGGGGTTTTTCTGTGCCGCATGTTATGTAAC
AGTCCGAGCATATTACAGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRLSRYFLPVLKETPSEAQVVSHRYMLRAGMIKQSAAGIYSWLPLGYRVLKKIEGIVHEEQMRAGHIPMLMPTIQSADLW
RESGRYDAYGEEMLRIRDRHDRDMLFTPTAEELITDIFRANVSSYKDLPLTMYQIQWKFRDEIRPRFGVMRGREFYMKDG
YNFDLTKEDALHAYNRHLVTYLRTYERMGLQAIPMRADGGPIGGDYTHEFLVLAETGESEVFYDSEITDLTFGAREIDYD
NVEQCQAVLEEFTSRYARTDETHDEALFNAVPEERRRVARGIEVGQIFYFGTKYSEALGATVQTADGQSVPVHMGSHGIG
VSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDDEADAACEQLYAALTAIGLEPLYDDRKERAGGKFASMDLIGL
PWRITVGPRGLKNGVVEVTSRRTGESEEMSPEDAVKKIAAIYANHPTPRGF

Sequences:

>Translated_451_residues
MRLSRYFLPVLKETPSEAQVVSHRYMLRAGMIKQSAAGIYSWLPLGYRVLKKIEGIVHEEQMRAGHIPMLMPTIQSADLW
RESGRYDAYGEEMLRIRDRHDRDMLFTPTAEELITDIFRANVSSYKDLPLTMYQIQWKFRDEIRPRFGVMRGREFYMKDG
YNFDLTKEDALHAYNRHLVTYLRTYERMGLQAIPMRADGGPIGGDYTHEFLVLAETGESEVFYDSEITDLTFGAREIDYD
NVEQCQAVLEEFTSRYARTDETHDEALFNAVPEERRRVARGIEVGQIFYFGTKYSEALGATVQTADGQSVPVHMGSHGIG
VSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDDEADAACEQLYAALTAIGLEPLYDDRKERAGGKFASMDLIGL
PWRITVGPRGLKNGVVEVTSRRTGESEEMSPEDAVKKIAAIYANHPTPRGF
>Mature_451_residues
MRLSRYFLPVLKETPSEAQVVSHRYMLRAGMIKQSAAGIYSWLPLGYRVLKKIEGIVHEEQMRAGHIPMLMPTIQSADLW
RESGRYDAYGEEMLRIRDRHDRDMLFTPTAEELITDIFRANVSSYKDLPLTMYQIQWKFRDEIRPRFGVMRGREFYMKDG
YNFDLTKEDALHAYNRHLVTYLRTYERMGLQAIPMRADGGPIGGDYTHEFLVLAETGESEVFYDSEITDLTFGAREIDYD
NVEQCQAVLEEFTSRYARTDETHDEALFNAVPEERRRVARGIEVGQIFYFGTKYSEALGATVQTADGQSVPVHMGSHGIG
VSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDDEADAACEQLYAALTAIGLEPLYDDRKERAGGKFASMDLIGL
PWRITVGPRGLKNGVVEVTSRRTGESEEMSPEDAVKKIAAIYANHPTPRGF

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=428, Percent_Identity=32.7102803738318, Blast_Score=231, Evalue=9e-61,
Organism=Escherichia coli, GI1786392, Length=229, Percent_Identity=54.1484716157205, Blast_Score=261, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI115532348, Length=430, Percent_Identity=31.1627906976744, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI193203271, Length=91, Percent_Identity=34.0659340659341, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=204, Percent_Identity=34.8039215686275, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24656200, Length=454, Percent_Identity=29.7356828193833, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_SILST (Q1GFI1)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613847.1
- ProteinModelPortal:   Q1GFI1
- SMR:   Q1GFI1
- STRING:   Q1GFI1
- GeneID:   4077877
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1852
- NMPDR:   fig|292414.1.peg.137
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- BioCyc:   SSP292414:TM1040_1852-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 51132; Mature: 51132

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPVLKETPSEAQVVSHRYMLRAGMIKQSAAGIYSWLPLGYRVLKKIEGIVHEE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMRAGHIPMLMPTIQSADLWRESGRYDAYGEEMLRIRDRHDRDMLFTPTAEELITDIFRA
HHHCCCCCEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
NVSSYKDLPLTMYQIQWKFRDEIRPRFGVMRGREFYMKDGYNFDLTKEDALHAYNRHLVT
CHHCCCCCCEEEEEEEEHHHHHCCHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
YLRTYERMGLQAIPMRADGGPIGGDYTHEFLVLAETGESEVFYDSEITDLTFGAREIDYD
HHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCCEEECCCCCCCCC
NVEQCQAVLEEFTSRYARTDETHDEALFNAVPEERRRVARGIEVGQIFYFGTKYSEALGA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCCCEEEEECCHHHHHHCC
TVQTADGQSVPVHMGSHGIGVSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDDE
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECEEEECCCCCH
ADAACEQLYAALTAIGLEPLYDDRKERAGGKFASMDLIGLPWRITVGPRGLKNGVVEVTS
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCEEEEECCCCHHCCCEEEEC
RRTGESEEMSPEDAVKKIAAIYANHPTPRGF
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLSRYFLPVLKETPSEAQVVSHRYMLRAGMIKQSAAGIYSWLPLGYRVLKKIEGIVHEE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMRAGHIPMLMPTIQSADLWRESGRYDAYGEEMLRIRDRHDRDMLFTPTAEELITDIFRA
HHHCCCCCEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
NVSSYKDLPLTMYQIQWKFRDEIRPRFGVMRGREFYMKDGYNFDLTKEDALHAYNRHLVT
CHHCCCCCCEEEEEEEEHHHHHCCHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
YLRTYERMGLQAIPMRADGGPIGGDYTHEFLVLAETGESEVFYDSEITDLTFGAREIDYD
HHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCCEEECCCCCCCCC
NVEQCQAVLEEFTSRYARTDETHDEALFNAVPEERRRVARGIEVGQIFYFGTKYSEALGA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCCCEEEEECCHHHHHHCC
TVQTADGQSVPVHMGSHGIGVSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDDE
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECEEEECCCCCH
ADAACEQLYAALTAIGLEPLYDDRKERAGGKFASMDLIGLPWRITVGPRGLKNGVVEVTS
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCEEEEECCCCHHCCCEEEEC
RRTGESEEMSPEDAVKKIAAIYANHPTPRGF
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA