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The map label for this gene is sir1 [H]

Identifier: 99081599

GI number: 99081599

Start: 1848717

End: 1850384

Strand: Direct

Name: sir1 [H]

Synonym: TM1040_1758

Alternate gene names: 99081599

Gene position: 1848717-1850384 (Clockwise)

Preceding gene: 99081598

Following gene: 99081600

Centisome position: 57.76

GC content: 61.99

Gene sequence:

>1668_bases
ATGTATAAGTACAACGACTTCGACTCCGCCTTTCTCGCAGAGCGCAATCGCCAGTTCCGTGCCCAGGTGGAGCGCCGCAT
CGCGGGCGCGCTGACCGAGGATGAGTTCAAGCCGCTGCGCCTGATGAACGGTCTCTATCTTCAGCTGCACGCCTATATGC
TGCGGGTTGCGATCCCCTATGGCACGCTGAACCCCGATCAGATGCGCGCGCTCGCACTGCTCGCTGAGAAGTGGGACAAG
GGCTACGGCCATTTCACCACGCGTCAGAACATCCAGTACAACTGGCCCAAACTGAAGGATGTGCCGGACATGCTGGACGC
GCTGGCAGAGGTGAACCTGCATGCGATCCAGACCTCGGGCAACACGATCCGTAACGTGACGGCCGACCATTTCGCCGGCG
CTGCCGCAGATGAGATCGAAGATCCCCGCCCCATCGCAGAGCTGATCCGCCAGTGGTCCACCGATCACCCGGAGTTTCAG
TTCATGGGCCGTAAGTTCAAGATCGCCGTCTCCGGGGCCGCCAACGATCGCGCCGTCCTGAAGGCACATGACTTGGCGAT
CAAGATCGTCAAGAACGAGGACGGCGAAGTGGGCTACCAGGTGCTGGTCGGCGGTGGCCTTGGCCGTACGCCGATGATCG
GCAAGGTGCTCAATGACTTTGTCGCCGCCGATGACCTCCTGCCCTACATCGAGAGCGTGATCTCGGTCTGGAACCAGCTT
GGCCGTCGCGACAACAAGTACAAGGCCCGCATCAAGATCACCGTGCACGAGCTCGGACTGGAGAAGATCCGCGAGCTGAC
CGAAGAGCGTTTTGCGCAAGTGCGCCCGACCTTTGGCGGCGCAGATCAAGCGTTGTTTCAGGACATCAAGCGCCACTTTG
CCGCGCCCGAGTTCCGCAGCGAAGGCACCGAGGTCTTTGACGCCGCTTATGACTCCGATCCTGTGTTCCGCGCCTGGGCC
GACACCAACCTCGCCGAACACAAGGCGCCGGGGCATGCGATCGCGACCATCTCGATCAAGAAACATGGCGCCACGCCGGG
CGATGCAACCGCCGCGCAGATGTACGCCATGGCAGATATCGCCGAAGAGTTCGCCTATGGCGAGCTGCGCATCAGCCACG
AGCAGAACGTGATCCTGCCGCATGTGCACAAGAACGATCTGCCCGCAATCCACGCGCGTCTGAAGGCGGCGGATCTGGCG
ACCGCGAACATCGGCCTGATCTCGGACATCATTGCCTGCCCGGGCATGGATTACTGCGCGCTGGCCACCGCCCGCTCGAT
CCCCGTGGCGCAGGAAATCGCCACCCGGTTCGAGGACCTGAAACTCGAGCACGACATTGGCCATCTGCAGATCAAGATCT
CTGGCTGCATCAACGCCTGCGGCCACCACCATGTCGGACACATCGGCATCCTTGGTCTGGATCGCGCGGGCGTCGAGAAC
TATCAGATCACGCTGGGTGGGGATGCGACCGAAACTGCAGCCATCGGCACGCGGACCGGACCGGGCTTTGCCTATGACGA
AATCGTCCCCGCGGTGGAGCGCATCGTCGAAACCTATCTGGAGCAACGCGAGAGCGCCGAAGAAGAGTTCCTGCAAACCT
ACCGCCGCGTCGGCATGGAGCCCTTCAAGGCGGCGCTCTACCCGGCCTCCGAGAAAAAGGTGGCCTGA

Upstream 100 bases:

>100_bases
CAGGGTCCGGAACCCACTCATTTCCGCGAAGAATTCCGCCGCACCGGCCCGTCGAACTATGCCCACGGCAAGCAGGAAGC
CAAACTGGAGGCCACCGCAA

Downstream 100 bases:

>100_bases
GGGATGACCCGTATAGATCAGGAGATCAGGGGCCATCCCGCCGCTGCTGGCAGCGCTGAGGCCCTGGCCGAGCAGGTTGC
GGCGCTCAATGCCCGCTTCA

Product: nitrite/sulfite reductase, hemoprotein beta-component, ferrodoxin-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MYKYNDFDSAFLAERNRQFRAQVERRIAGALTEDEFKPLRLMNGLYLQLHAYMLRVAIPYGTLNPDQMRALALLAEKWDK
GYGHFTTRQNIQYNWPKLKDVPDMLDALAEVNLHAIQTSGNTIRNVTADHFAGAAADEIEDPRPIAELIRQWSTDHPEFQ
FMGRKFKIAVSGAANDRAVLKAHDLAIKIVKNEDGEVGYQVLVGGGLGRTPMIGKVLNDFVAADDLLPYIESVISVWNQL
GRRDNKYKARIKITVHELGLEKIRELTEERFAQVRPTFGGADQALFQDIKRHFAAPEFRSEGTEVFDAAYDSDPVFRAWA
DTNLAEHKAPGHAIATISIKKHGATPGDATAAQMYAMADIAEEFAYGELRISHEQNVILPHVHKNDLPAIHARLKAADLA
TANIGLISDIIACPGMDYCALATARSIPVAQEIATRFEDLKLEHDIGHLQIKISGCINACGHHHVGHIGILGLDRAGVEN
YQITLGGDATETAAIGTRTGPGFAYDEIVPAVERIVETYLEQRESAEEEFLQTYRRVGMEPFKAALYPASEKKVA

Sequences:

>Translated_555_residues
MYKYNDFDSAFLAERNRQFRAQVERRIAGALTEDEFKPLRLMNGLYLQLHAYMLRVAIPYGTLNPDQMRALALLAEKWDK
GYGHFTTRQNIQYNWPKLKDVPDMLDALAEVNLHAIQTSGNTIRNVTADHFAGAAADEIEDPRPIAELIRQWSTDHPEFQ
FMGRKFKIAVSGAANDRAVLKAHDLAIKIVKNEDGEVGYQVLVGGGLGRTPMIGKVLNDFVAADDLLPYIESVISVWNQL
GRRDNKYKARIKITVHELGLEKIRELTEERFAQVRPTFGGADQALFQDIKRHFAAPEFRSEGTEVFDAAYDSDPVFRAWA
DTNLAEHKAPGHAIATISIKKHGATPGDATAAQMYAMADIAEEFAYGELRISHEQNVILPHVHKNDLPAIHARLKAADLA
TANIGLISDIIACPGMDYCALATARSIPVAQEIATRFEDLKLEHDIGHLQIKISGCINACGHHHVGHIGILGLDRAGVEN
YQITLGGDATETAAIGTRTGPGFAYDEIVPAVERIVETYLEQRESAEEEFLQTYRRVGMEPFKAALYPASEKKVA
>Mature_555_residues
MYKYNDFDSAFLAERNRQFRAQVERRIAGALTEDEFKPLRLMNGLYLQLHAYMLRVAIPYGTLNPDQMRALALLAEKWDK
GYGHFTTRQNIQYNWPKLKDVPDMLDALAEVNLHAIQTSGNTIRNVTADHFAGAAADEIEDPRPIAELIRQWSTDHPEFQ
FMGRKFKIAVSGAANDRAVLKAHDLAIKIVKNEDGEVGYQVLVGGGLGRTPMIGKVLNDFVAADDLLPYIESVISVWNQL
GRRDNKYKARIKITVHELGLEKIRELTEERFAQVRPTFGGADQALFQDIKRHFAAPEFRSEGTEVFDAAYDSDPVFRAWA
DTNLAEHKAPGHAIATISIKKHGATPGDATAAQMYAMADIAEEFAYGELRISHEQNVILPHVHKNDLPAIHARLKAADLA
TANIGLISDIIACPGMDYCALATARSIPVAQEIATRFEDLKLEHDIGHLQIKISGCINACGHHHVGHIGILGLDRAGVEN
YQITLGGDATETAAIGTRTGPGFAYDEIVPAVERIVETYLEQRESAEEEFLQTYRRVGMEPFKAALYPASEKKVA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=519, Percent_Identity=22.1579961464355, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 61665; Mature: 61665

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKYNDFDSAFLAERNRQFRAQVERRIAGALTEDEFKPLRLMNGLYLQLHAYMLRVAIPY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GTLNPDQMRALALLAEKWDKGYGHFTTRQNIQYNWPKLKDVPDMLDALAEVNLHAIQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCEEEEECCC
NTIRNVTADHFAGAAADEIEDPRPIAELIRQWSTDHPEFQFMGRKFKIAVSGAANDRAVL
CEEECCCHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCHHCCCCEEEEEEECCCCCCEEE
KAHDLAIKIVKNEDGEVGYQVLVGGGLGRTPMIGKVLNDFVAADDLLPYIESVISVWNQL
EEECEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKITVHELGLEKIRELTEERFAQVRPTFGGADQALFQDIKRHFAAPEFRS
CCCCCCEEEEEEEEHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHC
EGTEVFDAAYDSDPVFRAWADTNLAEHKAPGHAIATISIKKHGATPGDATAAQMYAMADI
CCCHHHHHCCCCCCCEEEECCCCHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHH
AEEFAYGELRISHEQNVILPHVHKNDLPAIHARLKAADLATANIGLISDIIACPGMDYCA
HHHHCCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHH
LATARSIPVAQEIATRFEDLKLEHDIGHLQIKISGCINACGHHHVGHIGILGLDRAGVEN
HHHHCCCCHHHHHHHHHHHCEEECCCCEEEEEEEHHHHHCCCCCCCEEEEEECCCCCCCC
YQITLGGDATETAAIGTRTGPGFAYDEIVPAVERIVETYLEQRESAEEEFLQTYRRVGME
EEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PFKAALYPASEKKVA
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MYKYNDFDSAFLAERNRQFRAQVERRIAGALTEDEFKPLRLMNGLYLQLHAYMLRVAIPY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GTLNPDQMRALALLAEKWDKGYGHFTTRQNIQYNWPKLKDVPDMLDALAEVNLHAIQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCEEEEECCC
NTIRNVTADHFAGAAADEIEDPRPIAELIRQWSTDHPEFQFMGRKFKIAVSGAANDRAVL
CEEECCCHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCHHCCCCEEEEEEECCCCCCEEE
KAHDLAIKIVKNEDGEVGYQVLVGGGLGRTPMIGKVLNDFVAADDLLPYIESVISVWNQL
EEECEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKITVHELGLEKIRELTEERFAQVRPTFGGADQALFQDIKRHFAAPEFRS
CCCCCCEEEEEEEEHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHC
EGTEVFDAAYDSDPVFRAWADTNLAEHKAPGHAIATISIKKHGATPGDATAAQMYAMADI
CCCHHHHHCCCCCCCEEEECCCCHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHH
AEEFAYGELRISHEQNVILPHVHKNDLPAIHARLKAADLATANIGLISDIIACPGMDYCA
HHHHCCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHH
LATARSIPVAQEIATRFEDLKLEHDIGHLQIKISGCINACGHHHVGHIGILGLDRAGVEN
HHHHCCCCHHHHHHHHHHHCEEECCCCEEEEEEEHHHHHCCCCCCCEEEEEECCCCCCCC
YQITLGGDATETAAIGTRTGPGFAYDEIVPAVERIVETYLEQRESAEEEFLQTYRRVGME
EEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PFKAALYPASEKKVA
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA