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The map label for this gene is gloA [H]

Identifier: 99081571

GI number: 99081571

Start: 1826199

End: 1826627

Strand: Direct

Name: gloA [H]

Synonym: TM1040_1730

Alternate gene names: 99081571

Gene position: 1826199-1826627 (Clockwise)

Preceding gene: 99081568

Following gene: 99081575

Centisome position: 57.05

GC content: 58.74

Gene sequence:

>429_bases
ATGCCACTCAAGTATCTGCATACAATGGTCCGGGTCAAAGACCTGGAGAAATCCATGGCGTTCTATGCGCTGCTCGGCCT
CAAAGAAACCCGCCGCTACGACAGCGAGGCAGGACGGTTTTCGCTGATCTTCATGGCCCCTCCCGGCCAGGAGGAGGCTC
CGGTCGAGCTCACCTGGAACTGGGATGGCGACGACGAACTGCCGTCTGACAGCCGCCACTTTGGCCACCTCGCTTATGAA
GTCGACAATATCTACGAGACCTGCCAGCACCTCATGGACAACGGTGTTGTCATTAACCGTCCGCCCCGCGATGGGCGGAT
GGCGTTTGTGCGCTCACCCGACAATATCTCGATCGAATTGCTGCAGGCGGGGGATGCACTTCCACCGGCAGAGCCTTGGT
CCAGCATGGAAAACACCGGTCACTGGTGA

Upstream 100 bases:

>100_bases
AGCATCGCAGAGTTCCAAGCGGTTTACGCGCGCTCTCGACATTCTGGACGCGCGGCTTAGGTTGGGCGTTGAATTCGCAC
AAATGCAAGGGGATGCCTTC

Downstream 100 bases:

>100_bases
TCCGATGCGCGCCGTGTGTGAGGGGGCACGCTGCCGCGTCCCCCGTGGCGCCTTCTAACGTGGCCCGGTCTAAACTGGCC
CGGTCTAAACTGGAAAGTCG

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MPLKYLHTMVRVKDLEKSMAFYALLGLKETRRYDSEAGRFSLIFMAPPGQEEAPVELTWNWDGDDELPSDSRHFGHLAYE
VDNIYETCQHLMDNGVVINRPPRDGRMAFVRSPDNISIELLQAGDALPPAEPWSSMENTGHW

Sequences:

>Translated_142_residues
MPLKYLHTMVRVKDLEKSMAFYALLGLKETRRYDSEAGRFSLIFMAPPGQEEAPVELTWNWDGDDELPSDSRHFGHLAYE
VDNIYETCQHLMDNGVVINRPPRDGRMAFVRSPDNISIELLQAGDALPPAEPWSSMENTGHW
>Mature_141_residues
PLKYLHTMVRVKDLEKSMAFYALLGLKETRRYDSEAGRFSLIFMAPPGQEEAPVELTWNWDGDDELPSDSRHFGHLAYEV
DNIYETCQHLMDNGVVINRPPRDGRMAFVRSPDNISIELLQAGDALPPAEPWSSMENTGHW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=142, Percent_Identity=34.5070422535211, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=40.1515151515151, Blast_Score=86, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323639, Length=144, Percent_Identity=31.25, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129743, Length=147, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16250; Mature: 16119

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKYLHTMVRVKDLEKSMAFYALLGLKETRRYDSEAGRFSLIFMAPPGQEEAPVELTWN
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEC
WDGDDELPSDSRHFGHLAYEVDNIYETCQHLMDNGVVINRPPRDGRMAFVRSPDNISIEL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCEEEE
LQAGDALPPAEPWSSMENTGHW
EECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLKYLHTMVRVKDLEKSMAFYALLGLKETRRYDSEAGRFSLIFMAPPGQEEAPVELTWN
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEC
WDGDDELPSDSRHFGHLAYEVDNIYETCQHLMDNGVVINRPPRDGRMAFVRSPDNISIEL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCEEEE
LQAGDALPPAEPWSSMENTGHW
EECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]