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The map label for this gene is pinE [H]

Identifier: 99081486

GI number: 99081486

Start: 1750371

End: 1751012

Strand: Reverse

Name: pinE [H]

Synonym: TM1040_1645

Alternate gene names: 99081486

Gene position: 1751012-1750371 (Counterclockwise)

Preceding gene: 99081488

Following gene: 99081479

Centisome position: 54.7

GC content: 63.55

Gene sequence:

>642_bases
GTGGCGTCCGAATCCGAGAACCAGCCGACACGCAAGATCGGATACGCTCGCGTCTCCACTGCCGACCAAAACCCGGATAT
GCAGATCCATGAGCTGAAGCGGTACGGCGTCCCAGAGGAATTGATCTTTGTGGACCGGGCCAGCGGCGGCACCATGAAGC
GACCCCAGTTCATGAGGGCGCTGAGGTATGCCCAATATGAAGGGACGGAGCTGGTCGTCTGGAAGCTGGACCGCCTTGGG
CGGACCCTTGGCGGCGTGCTGGATACGCTCAAGCTGCTGTCAGACCGCGGCGTTACCTTCGTCAGCCTGACGGAGCGGAT
CGACACCACGGGCCCTATGGGCAAGGCTATGATCCACCTTCTGGCCGTCTTCGCCGAACTGGAGCGAGACCTGATCGTGG
AGCGCACCAAGGCCGGGATCGAGCGGGCGAAAGAGCGCGGCGACGTGGGTGGCCGACCAAAGGCTATGACGCCGGAGCGG
GTTGCCAAGGCAGAGGAAATGCTTGCCGCTGGTGAGCGCGGCAACACGGTCTGGAAGGCGATGAAGGAACTCGATGGGCC
GACGATCAGCCGGGCGGCATACTACGCCTGGCAGAAGGAATGGGACGCGGGCCACCGCGCCCCGGATATCGTTGGAGAGT
GA

Upstream 100 bases:

>100_bases
GTAACAAGACTATGTTTCTGGACTGTATCGCGCTGCAGTTGACCTGTCTAGATAGTTAGTCCAGATTAGGCGTGAAAATT
TTACGCGAGGTAGTAAGAAA

Downstream 100 bases:

>100_bases
TCCAGAAAAGGAGCGAGTCTATGTGCGTGACGGCTTGGCTAGGGGATAAATGCCTGGAGACGGTCGGAGACGTCCGAGCC
GCGCTTGGATCTGTCGTAAT

Product: resolvase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MASESENQPTRKIGYARVSTADQNPDMQIHELKRYGVPEELIFVDRASGGTMKRPQFMRALRYAQYEGTELVVWKLDRLG
RTLGGVLDTLKLLSDRGVTFVSLTERIDTTGPMGKAMIHLLAVFAELERDLIVERTKAGIERAKERGDVGGRPKAMTPER
VAKAEEMLAAGERGNTVWKAMKELDGPTISRAAYYAWQKEWDAGHRAPDIVGE

Sequences:

>Translated_213_residues
MASESENQPTRKIGYARVSTADQNPDMQIHELKRYGVPEELIFVDRASGGTMKRPQFMRALRYAQYEGTELVVWKLDRLG
RTLGGVLDTLKLLSDRGVTFVSLTERIDTTGPMGKAMIHLLAVFAELERDLIVERTKAGIERAKERGDVGGRPKAMTPER
VAKAEEMLAAGERGNTVWKAMKELDGPTISRAAYYAWQKEWDAGHRAPDIVGE
>Mature_212_residues
ASESENQPTRKIGYARVSTADQNPDMQIHELKRYGVPEELIFVDRASGGTMKRPQFMRALRYAQYEGTELVVWKLDRLGR
TLGGVLDTLKLLSDRGVTFVSLTERIDTTGPMGKAMIHLLAVFAELERDLIVERTKAGIERAKERGDVGGRPKAMTPERV
AKAEEMLAAGERGNTVWKAMKELDGPTISRAAYYAWQKEWDAGHRAPDIVGE

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=159, Percent_Identity=49.0566037735849, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1787638, Length=178, Percent_Identity=33.7078651685393, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1787827, Length=175, Percent_Identity=33.7142857142857, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23835; Mature: 23703

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASESENQPTRKIGYARVSTADQNPDMQIHELKRYGVPEELIFVDRASGGTMKRPQFMRA
CCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHEEEECCCCCCCCCHHHHHH
LRYAQYEGTELVVWKLDRLGRTLGGVLDTLKLLSDRGVTFVSLTERIDTTGPMGKAMIHL
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCHHHHHHHH
LAVFAELERDLIVERTKAGIERAKERGDVGGRPKAMTPERVAKAEEMLAAGERGNTVWKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
MKELDGPTISRAAYYAWQKEWDAGHRAPDIVGE
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ASESENQPTRKIGYARVSTADQNPDMQIHELKRYGVPEELIFVDRASGGTMKRPQFMRA
CCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHEEEECCCCCCCCCHHHHHH
LRYAQYEGTELVVWKLDRLGRTLGGVLDTLKLLSDRGVTFVSLTERIDTTGPMGKAMIHL
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCHHHHHHHH
LAVFAELERDLIVERTKAGIERAKERGDVGGRPKAMTPERVAKAEEMLAAGERGNTVWKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
MKELDGPTISRAAYYAWQKEWDAGHRAPDIVGE
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]