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The map label for this gene is dam [H]

Identifier: 99081428

GI number: 99081428

Start: 1695177

End: 1696058

Strand: Reverse

Name: dam [H]

Synonym: TM1040_1587

Alternate gene names: 99081428

Gene position: 1696058-1695177 (Counterclockwise)

Preceding gene: 99081429

Following gene: 99081426

Centisome position: 52.99

GC content: 59.41

Gene sequence:

>882_bases
ATGGCAATAGAGATACCAGTTCCAGCCGTCGCGCCCGCTTTGCCAAAACCCTTCAAGACGCAGCTTCTCAAATGGGTGGG
CAACAAGCAGCGCTTTGCCCATGAAATCGTTGCACATCTTCCCAAGGACTACGGCACCTATTTTGAACCGTTTCTGGGCG
CAGGCGGGGTGATGGCGAACCTGCAACCACATCGCGCGCTGGGCTCAGACATCTATCCTCCCCTGGTCGAGATCTGGCAG
GCGCTGCGCGAGGACCCCGCGCGCCTCAACGCGTGGTATCAGAGCCGCTATGACCGCTTCATGACCGGTGACAGGGTTGC
CACCTATGAGGCGATCAAGGCCCGCTACAATGACACCCCCAACGGTGCCGATTTCCTGTTTCTCTGCCGCGCCTGTTACG
GTGGGGTGGTGCGCTTTCGTCGCAAGGACGGATATATGTCGACCCCCTGCGGCAGCCACCCGGTGATGAAACCTCACAAA
TTTGCCGAACGGGTCGAGATGTGGCGCGCGCGCATGAAAGGCGCAAGTTTTGCCTGTCTGGACTACCGCTCTGCCATGGC
GCAGGCGCGCGCGGGCGATGTGATTTACTGCGACCCGCCTTACGCTCATTCGCAAACCATCCTTTACGGCGCCCAGGCGT
TTCGCCTCGAAGAATTGTTTGCGGCGATCGCGGCCTGCAAGGCACGCGGCGTCAGGGTCGCGCTTTCGATCGATGGCACC
AAGAAATCCGGCGATATCTATTGCGACATCGCCATCCCTGAGGGTCTGTTTGAACGCGAGATCCTCGTCAATATGGGGCA
ATCCATGCTCAAACGCTTTCAGATGAGCGGGCAGCGCATGGAGAGCGAAGCCGTGCGCGACCGCCTGCTTTTGACCTACT
AA

Upstream 100 bases:

>100_bases
GCAGGTGCCCCCTTTTTTTGTCAGCCCGCGTCCCACTGGCAGATTCCGCGCGGATAGCGTATGCGAAACACACCGTGCAT
CAAGAAACCAGAGGGAGCGG

Downstream 100 bases:

>100_bases
GTCCTTCTTCTAACGAGAAATATCCCGGGGGAGGCCCGACAAGGGCCGGGGGCAGCGCCCCCAGGGCTTCTGCGCTGCGT
TCACGCGTTCAAGAAACCGC

Product: DNA adenine methylase Dam

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.VchADam [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MAIEIPVPAVAPALPKPFKTQLLKWVGNKQRFAHEIVAHLPKDYGTYFEPFLGAGGVMANLQPHRALGSDIYPPLVEIWQ
ALREDPARLNAWYQSRYDRFMTGDRVATYEAIKARYNDTPNGADFLFLCRACYGGVVRFRRKDGYMSTPCGSHPVMKPHK
FAERVEMWRARMKGASFACLDYRSAMAQARAGDVIYCDPPYAHSQTILYGAQAFRLEELFAAIAACKARGVRVALSIDGT
KKSGDIYCDIAIPEGLFEREILVNMGQSMLKRFQMSGQRMESEAVRDRLLLTY

Sequences:

>Translated_293_residues
MAIEIPVPAVAPALPKPFKTQLLKWVGNKQRFAHEIVAHLPKDYGTYFEPFLGAGGVMANLQPHRALGSDIYPPLVEIWQ
ALREDPARLNAWYQSRYDRFMTGDRVATYEAIKARYNDTPNGADFLFLCRACYGGVVRFRRKDGYMSTPCGSHPVMKPHK
FAERVEMWRARMKGASFACLDYRSAMAQARAGDVIYCDPPYAHSQTILYGAQAFRLEELFAAIAACKARGVRVALSIDGT
KKSGDIYCDIAIPEGLFEREILVNMGQSMLKRFQMSGQRMESEAVRDRLLLTY
>Mature_292_residues
AIEIPVPAVAPALPKPFKTQLLKWVGNKQRFAHEIVAHLPKDYGTYFEPFLGAGGVMANLQPHRALGSDIYPPLVEIWQA
LREDPARLNAWYQSRYDRFMTGDRVATYEAIKARYNDTPNGADFLFLCRACYGGVVRFRRKDGYMSTPCGSHPVMKPHKF
AERVEMWRARMKGASFACLDYRSAMAQARAGDVIYCDPPYAHSQTILYGAQAFRLEELFAAIAACKARGVRVALSIDGTK
KSGDIYCDIAIPEGLFEREILVNMGQSMLKRFQMSGQRMESEAVRDRLLLTY

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=206, Percent_Identity=28.6407766990291, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 33080; Mature: 32948

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIPVPAVAPALPKPFKTQLLKWVGNKQRFAHEIVAHLPKDYGTYFEPFLGAGGVMAN
CEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
LQPHRALGSDIYPPLVEIWQALREDPARLNAWYQSRYDRFMTGDRVATYEAIKARYNDTP
CCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
NGADFLFLCRACYGGVVRFRRKDGYMSTPCGSHPVMKPHKFAERVEMWRARMKGASFACL
CCHHHHHHHHHHHHHHHEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHH
DYRSAMAQARAGDVIYCDPPYAHSQTILYGAQAFRLEELFAAIAACKARGVRVALSIDGT
HHHHHHHHHHCCCEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
KKSGDIYCDIAIPEGLFEREILVNMGQSMLKRFQMSGQRMESEAVRDRLLLTY
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIEIPVPAVAPALPKPFKTQLLKWVGNKQRFAHEIVAHLPKDYGTYFEPFLGAGGVMAN
EEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
LQPHRALGSDIYPPLVEIWQALREDPARLNAWYQSRYDRFMTGDRVATYEAIKARYNDTP
CCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
NGADFLFLCRACYGGVVRFRRKDGYMSTPCGSHPVMKPHKFAERVEMWRARMKGASFACL
CCHHHHHHHHHHHHHHHEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHH
DYRSAMAQARAGDVIYCDPPYAHSQTILYGAQAFRLEELFAAIAACKARGVRVALSIDGT
HHHHHHHHHHCCCEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
KKSGDIYCDIAIPEGLFEREILVNMGQSMLKRFQMSGQRMESEAVRDRLLLTY
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11705940 [H]