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The map label for this gene is braE [H]

Identifier: 99081354

GI number: 99081354

Start: 1620001

End: 1621200

Strand: Direct

Name: braE [H]

Synonym: TM1040_1513

Alternate gene names: 99081354

Gene position: 1620001-1621200 (Clockwise)

Preceding gene: 99081353

Following gene: 99081355

Centisome position: 50.61

GC content: 58.42

Gene sequence:

>1200_bases
ATGTTCAAACTGGAAAAAACCGATCTCAAACTTCTCATGATCGTGGCTGTTCTGACCATGTTCGCCCCGTTCATCCTGAA
CCCCTTCCCCGTCGACAGCGCCATGGCGCAGTTCAACGCGGGCTATCCGGACCTGATGCAGCGCTTTGTGATCTTTGGTG
TCTTTGCCATCGGGTTCAACATCCTCTTTGGCCTCACCGGCTACCTCTCCTTTGGTCACGCGGCCTTTCTCGGGGTGGGG
TCCTATGCGGCCGTCTGGATGTTCAAACTGCTGAGCATGAACGTGCTGCCCGCCATCGTGCTGTCGGTTCTGGTCTCCGG
TCTCTTTTCGCTGGTGATTGGCTTTGTGAGCCTGCGGCGCTCGGGGATCTACTTCTCCATTCTGACTCTGGCCTTTGCCC
AGATGATGTTTGCGCTGGCCTATTCGGTTCTGACCCCGATCACGGGCGGTGAAACGGGCCTGCAGTTGGCGCTGGATGAT
CCGCGCATTCTCGGCGCCAGCCAGACCGCCGAGGGCAATATCCCGGTCCCGAGCCTCTTTGGGCTGGAGATGCGCGACAG
TGCAGAACTGGTGATCGGCGGCTGGTCCTTTACCTTCAACTTTGGCTACTACTTCTGCGCGCTGGTGATGCTTGCATCGT
TCTATCTCGCGATCCGCATCTTCCGCTCGCCCTTTGGGATGATGCTGCGGGCGGTGAAATCCAACCAGCAGCGGATGAAC
TACACTGGTCTCAACTCCCGTCCCTATATGCTTGCGGCCTTTGTGATCTCTGGCATGTACGCGGGGCTCGCCGGTGGTCT
CATGGCCTCCATGGACCCGCTGGCTGGCGCGGAGCGGATGCAGTGGACCGCATCGGGCGAAGTCGTTCTGATGACCATCC
TTGGTGGGGCTGGCACGCTGATCGGTCCGGTTCTGGGCGCAGGCTTCATCAAATACTTCGAGAACATCTTCTCCAAGATC
AACGACAACGTGCTGCACAGCTGGTTCAGCTTCCTGCCGGATGGGCTTGAGGACTTTGTGGTTTTCATCATCCACCCCTT
TATCGGCAAGGGCTGGCATCTGACGCTTGGCATCCTCTTCATGCTGGTGGTGATCTACCTCCCCGGCGGACTTGTGGAAG
GCGGACAGCGGGTCGGAAACTGGCTCAAGGGGCGCAAGAAGTCTGACGCCAAGCAGAGCAACACCAATCCGGCGGAATAA

Upstream 100 bases:

>100_bases
GGCATCAACCAGGTCATCATCTATCTGGTCGCCATCATCATTCTTCTCACCCGCCCCCGTGGATTGATGGGCCGCAAAGG
CGTGATGGAGGACTAAGGAC

Downstream 100 bases:

>100_bases
GGAGACCCTCATGAGCATTCTCGAAGTCAAAAACGTCGGCAAACGCTTTGGTGGCCTCCAGGCGCTGTCGGATGTGAACC
TCTCCGTCAAGGAGAACTCG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MFKLEKTDLKLLMIVAVLTMFAPFILNPFPVDSAMAQFNAGYPDLMQRFVIFGVFAIGFNILFGLTGYLSFGHAAFLGVG
SYAAVWMFKLLSMNVLPAIVLSVLVSGLFSLVIGFVSLRRSGIYFSILTLAFAQMMFALAYSVLTPITGGETGLQLALDD
PRILGASQTAEGNIPVPSLFGLEMRDSAELVIGGWSFTFNFGYYFCALVMLASFYLAIRIFRSPFGMMLRAVKSNQQRMN
YTGLNSRPYMLAAFVISGMYAGLAGGLMASMDPLAGAERMQWTASGEVVLMTILGGAGTLIGPVLGAGFIKYFENIFSKI
NDNVLHSWFSFLPDGLEDFVVFIIHPFIGKGWHLTLGILFMLVVIYLPGGLVEGGQRVGNWLKGRKKSDAKQSNTNPAE

Sequences:

>Translated_399_residues
MFKLEKTDLKLLMIVAVLTMFAPFILNPFPVDSAMAQFNAGYPDLMQRFVIFGVFAIGFNILFGLTGYLSFGHAAFLGVG
SYAAVWMFKLLSMNVLPAIVLSVLVSGLFSLVIGFVSLRRSGIYFSILTLAFAQMMFALAYSVLTPITGGETGLQLALDD
PRILGASQTAEGNIPVPSLFGLEMRDSAELVIGGWSFTFNFGYYFCALVMLASFYLAIRIFRSPFGMMLRAVKSNQQRMN
YTGLNSRPYMLAAFVISGMYAGLAGGLMASMDPLAGAERMQWTASGEVVLMTILGGAGTLIGPVLGAGFIKYFENIFSKI
NDNVLHSWFSFLPDGLEDFVVFIIHPFIGKGWHLTLGILFMLVVIYLPGGLVEGGQRVGNWLKGRKKSDAKQSNTNPAE
>Mature_399_residues
MFKLEKTDLKLLMIVAVLTMFAPFILNPFPVDSAMAQFNAGYPDLMQRFVIFGVFAIGFNILFGLTGYLSFGHAAFLGVG
SYAAVWMFKLLSMNVLPAIVLSVLVSGLFSLVIGFVSLRRSGIYFSILTLAFAQMMFALAYSVLTPITGGETGLQLALDD
PRILGASQTAEGNIPVPSLFGLEMRDSAELVIGGWSFTFNFGYYFCALVMLASFYLAIRIFRSPFGMMLRAVKSNQQRMN
YTGLNSRPYMLAAFVISGMYAGLAGGLMASMDPLAGAERMQWTASGEVVLMTILGGAGTLIGPVLGAGFIKYFENIFSKI
NDNVLHSWFSFLPDGLEDFVVFIIHPFIGKGWHLTLGILFMLVVIYLPGGLVEGGQRVGNWLKGRKKSDAKQSNTNPAE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=297, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 43541; Mature: 43541

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]