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The map label for this gene is paaF [C]

Identifier: 99081277

GI number: 99081277

Start: 1532091

End: 1532918

Strand: Reverse

Name: paaF [C]

Synonym: TM1040_1436

Alternate gene names: 99081277

Gene position: 1532918-1532091 (Counterclockwise)

Preceding gene: 99081278

Following gene: 99081276

Centisome position: 47.89

GC content: 60.75

Gene sequence:

>828_bases
ATGGCCAAAGACGATATTGCCAACAACAACATCGCAGAGCCGCTGATCACGCGACACGATGATGCCGGCGTTGCGCGGCT
CACCCTGAACCAGCCGCGCCGGATCAACGCGCTCTCGGAGGCGATGCTCCATGCGCTGCAGGCGGCACTGGATGAAATCG
CCGAGGATCGCAGCGTGCGAGCGGTGATCCTCAGTGGCGCTGGGGATCATTTTTGCGCCGGTCACAACCTAAAGGAAATG
ACCGAAGCGCGCGCTGGTGACGATGGCGGCTTTGAGTATTTCCAACAGCTTTTTGCAACCTGCTCGCGGATGATGCAGAC
CATTGTGGAGCTGCCGCAGCCGGTCATTGCCGAGGTGTCGGGGATCGCGACTGCTGCAGGCTGTCAACTGGTTGCCACCT
GCGATCTTGCGGTCGCTTCCGAGGATGCGCGGTTTGCGACCTCCGGGGTGACGATTGGTCTGTTCTGTTCCACGCCGATG
GTGGCGCTGACCCGCAATCTATTGCGCAAACATGCGATGGAGATGCTCTTGCTTGGCGAATTTATCCCGGCCTCCCGTGC
GGCCGAGATCGGACTGGTCAACCGCGTGGTGCCGCTTTCAGATCTCGCGCAAGAAACCACCGAACTGGCGCGTCGCATCG
CCGGCAAATCCCCAGCCGCGATCAAGATCGGCAAATCGGCCTTTTATGAGCAGGCGCAGATGTCGCTGGCCGAGGCCTAT
GCCTGCACCGGTCGTGCCATGGCCGAAAACATGATGGCGCGAGATGCCGAGGCGGGTATTGGCGCATTTCTCTCCAAAGC
CCCTATGCCGGATTGGACAGGCGAGTAG

Upstream 100 bases:

>100_bases
GACCCAAGAGGCGATTTCGGAATTTGGTCTCGGCTGAGGCTTCAGGCTTGGCGGTGCGAGGTTGCTGATCTAGGCTTTGG
TCTGCGACAGGAGGTGCCCC

Downstream 100 bases:

>100_bases
GGCGTGGCCTGGTTACGTCGAGTTTGACCCGCAGGCGTAACGCTATGCAATCGCGCGATTCGAGCACTTTGTGCCACGTT
CAGTGAACAAAGGCGTCAAA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MAKDDIANNNIAEPLITRHDDAGVARLTLNQPRRINALSEAMLHALQAALDEIAEDRSVRAVILSGAGDHFCAGHNLKEM
TEARAGDDGGFEYFQQLFATCSRMMQTIVELPQPVIAEVSGIATAAGCQLVATCDLAVASEDARFATSGVTIGLFCSTPM
VALTRNLLRKHAMEMLLLGEFIPASRAAEIGLVNRVVPLSDLAQETTELARRIAGKSPAAIKIGKSAFYEQAQMSLAEAY
ACTGRAMAENMMARDAEAGIGAFLSKAPMPDWTGE

Sequences:

>Translated_275_residues
MAKDDIANNNIAEPLITRHDDAGVARLTLNQPRRINALSEAMLHALQAALDEIAEDRSVRAVILSGAGDHFCAGHNLKEM
TEARAGDDGGFEYFQQLFATCSRMMQTIVELPQPVIAEVSGIATAAGCQLVATCDLAVASEDARFATSGVTIGLFCSTPM
VALTRNLLRKHAMEMLLLGEFIPASRAAEIGLVNRVVPLSDLAQETTELARRIAGKSPAAIKIGKSAFYEQAQMSLAEAY
ACTGRAMAENMMARDAEAGIGAFLSKAPMPDWTGE
>Mature_274_residues
AKDDIANNNIAEPLITRHDDAGVARLTLNQPRRINALSEAMLHALQAALDEIAEDRSVRAVILSGAGDHFCAGHNLKEMT
EARAGDDGGFEYFQQLFATCSRMMQTIVELPQPVIAEVSGIATAAGCQLVATCDLAVASEDARFATSGVTIGLFCSTPMV
ALTRNLLRKHAMEMLLLGEFIPASRAAEIGLVNRVVPLSDLAQETTELARRIAGKSPAAIKIGKSAFYEQAQMSLAEAYA
CTGRAMAENMMARDAEAGIGAFLSKAPMPDWTGE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=46.2450592885375, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=29.9212598425197, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI260274832, Length=258, Percent_Identity=25.968992248062, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI45643119, Length=258, Percent_Identity=25.968992248062, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260275230, Length=258, Percent_Identity=25.968992248062, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI70995211, Length=224, Percent_Identity=28.125, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4502327, Length=273, Percent_Identity=26.3736263736264, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=232, Percent_Identity=25.8620689655172, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI25453481, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI51538573, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=269, Percent_Identity=33.0855018587361, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=31.0344827586207, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787660, Length=270, Percent_Identity=30, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=28.030303030303, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=27.3076923076923, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=24.8704663212435, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=26.9035532994924, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=41.6326530612245, Blast_Score=180, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=31.8897637795276, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=28.7937743190661, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=238, Percent_Identity=30.2521008403361, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=27.2030651340996, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=25.6704980842912, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=212, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=209, Percent_Identity=27.7511961722488, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=25.3456221198157, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=38.0769230769231, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI20129971, Length=265, Percent_Identity=29.0566037735849, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653477, Length=265, Percent_Identity=29.0566037735849, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653139, Length=298, Percent_Identity=27.8523489932886, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=30.0518134715026, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=25.7692307692308, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24650670, Length=270, Percent_Identity=25.1851851851852, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21357171, Length=185, Percent_Identity=25.9459459459459, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29327; Mature: 29196

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDDIANNNIAEPLITRHDDAGVARLTLNQPRRINALSEAMLHALQAALDEIAEDRSVR
CCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
AVILSGAGDHFCAGHNLKEMTEARAGDDGGFEYFQQLFATCSRMMQTIVELPQPVIAEVS
EEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GIATAAGCQLVATCDLAVASEDARFATSGVTIGLFCSTPMVALTRNLLRKHAMEMLLLGE
HHHHHHCCEEEEEHHHHHCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
FIPASRAAEIGLVNRVVPLSDLAQETTELARRIAGKSPAAIKIGKSAFYEQAQMSLAEAY
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHH
ACTGRAMAENMMARDAEAGIGAFLSKAPMPDWTGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AKDDIANNNIAEPLITRHDDAGVARLTLNQPRRINALSEAMLHALQAALDEIAEDRSVR
CCCCCCCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
AVILSGAGDHFCAGHNLKEMTEARAGDDGGFEYFQQLFATCSRMMQTIVELPQPVIAEVS
EEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GIATAAGCQLVATCDLAVASEDARFATSGVTIGLFCSTPMVALTRNLLRKHAMEMLLLGE
HHHHHHCCEEEEEHHHHHCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
FIPASRAAEIGLVNRVVPLSDLAQETTELARRIAGKSPAAIKIGKSAFYEQAQMSLAEAY
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHH
ACTGRAMAENMMARDAEAGIGAFLSKAPMPDWTGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA