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The map label for this gene is ligA

Identifier: 99081239

GI number: 99081239

Start: 1490751

End: 1492970

Strand: Reverse

Name: ligA

Synonym: TM1040_1398

Alternate gene names: 99081239

Gene position: 1492970-1490751 (Counterclockwise)

Preceding gene: 99081240

Following gene: 99081238

Centisome position: 46.64

GC content: 60.9

Gene sequence:

>2220_bases
ATGGATGAGGCCGACGCGCGCGCTGAGTTTGAGGCGCTGCAGACCAAGATCGAAAAAGCCGATCACGACTATCACCAAAA
AGACGCGCCCACTCTGTCGGATGCGGATTACGATCGCCTCAAGCGTCGCTATCTGGCCCTTGCAGACGCCTTTCCCATCT
TGGCCAAAGACGGCACCCGTGTAGCGAGCGTTGGCGCGCCGGCCGCCTCTGGCTTTGGCAAGGTCACCCATGCGCAACGT
ATGATGTCCCTCGGAAATGCGTTTGAAGATCAGGATGTTGAGGATTTTGCTGTCGGGTTGCGCCGCTATCTGGGGCTCTC
CTCCGAGGCTCCGCTCGCCTTCACCGCAGAGCCGAAAATTGACGGGCTTTCGCTTTCCCTGCGCTATGAGGCGGGCAAAC
TTGTGCAAGCTGCAACCCGCGGCGATGGCGCGGTGGGGGAAAACGTCACCGAAAACGCCCGCACCATCAGCGATATTCCG
CAAGAAATCTCTGGCGCGCCCGAGGTCCTGGAAGTGCGTGGCGAAGTCTACATGAGCCACGCAGATTTCGAGGCTCTGAA
TGCGCGTCATGCCGAGACCGGCGGCAAGATATTTGCCAACCCGCGCAATGCGGCGGCGGGCTCGCTGCGCCAGCTCGATG
CTGAAATCACCCGCGCCCGACCTTTGCGGTTCTTTGCCTATAGCTGGGGAGAGCTGTCTGAACCTCTCGCAGAAACGCAA
ATTGACGCGATCGAGAGACTGGCGTCCCTTGGCTTTCAGACCAATCCGCTGACACGCTGCTGCGAGCAGATTTCCGAGCT
GCTCGCGCATTATCACGCCATCGAGGAGCAGCGCGCGGATCTCGGCTATGACATAGACGGAGTTGTTTACAAGGTGAACG
ATCTGTCCTTGCAAGAGCGTCTTGGCTTTCGGTCAACGACGCCACGTTGGGCCATTGCGCATAAGTTTCCGGCCGAGCTG
GCCTGGACCCACCTTGAGGGGATCGACATTCAGGTTGGACGCACCGGCGCCCTGAGCCCGGTTGCGCGTCTGCATCCGGT
GACCGTGGGCGGTGTGGTTGTCTCCAACGCCACGTTGCACAACGAAGACTATATTGCGGGACTTGATTCCAAAGGCGCTC
CGATCCGGGGCGGCAAGGACATCCGCGTCGGGGACTGGGTGCAGATCTACCGCGCGGGCGATGTGATCCCAAAGGTCGCC
GACGTCGATCTGAGCCGACGCCCCGAGGGCACGGAGCGTTATGCGTTCCCCACGCGCTGTCCCCGATGCGACAGCCCCGC
GGTGCGCGAAGAAGGTGATGCTGTGCGACGTTGCAGCGGCGGGTTGATTTGCCCGGCGCAGGCGGTTGAGAAACTCAAGC
ATTTTGTCTCGCGCGCGGCTTTTGACATCGAAGGGCTGGGCGCCAAACAGGTGGAACAATTCCACAGCGATGGATGGGTG
AAGGAACCCGCCGATATCTTTGAGCTCCAACAGAGATATGGCTCTGGTTTGCAGCAGTTGAAAAACCGCGAAGGCTGGGG
GGAGAAATCTGCGTCTGCTCTGTTTGCAGCCATCGAGGACAAGCGGCGAATTGAGTTTGCCCGGCTCATTTTTGGCCTCG
GGATCCGCCATGTGGGAGAAGTTGCCGCCAAGGACCTTGCCCTGCATTTCCGGACCTGGAGCGCCCTGGCGGAGGCCGCG
GATCTCGCGCGCGCAGCGGCGTTGGCGCACCGGGCTGCGGATGAAGCCGAGATTGTTGAACGGCAGGAGGCACAAGCCAG
TGCGCGCCGTGCAAAAATCTCTGAGGCCCGAAATGCCGCGGTTGCAACCTGTGCAGTTGCGCCGGACTCGCAGGCGGCGT
GGGATGATCTCATCAGTGTGGATGGGCTTGGCCCAACCGTGGCTTTGTCTTTGTCGGATGCCTTTGCCAACCCCGAAGAA
CGCGCCGCTTTTGATCGTCTGATCGCGCATCTAGAGATTATTGAACCGGATGCCCCGGCAGATGACAGCCCCGTTGCGGG
CAAGACCGTCGTCTTTACCGGCACGCTCGAAAAAATGACCCGCGCCGAGGCAAAGGCGCGCGCCGAGGCGCTTGGGGCCA
AAGTGTCTGGGTCGGTGTCAAAGAAAACCGATATTCTTGTGGCCGGACCGGGCGCGGGGTCGAAGGCCGCCAAGGCTGCA
GAGCTCGGCATTCAGACATTGGATGAGGACGGATGGCTGGACTTGATCGGGCAGGCATAG

Upstream 100 bases:

>100_bases
TAGATCTGACACTGGACCTCTCTCATTGCGGGCACTATCACCATGGGCAGAACACTACCCAAAGGGCGGAGCCAGAATGA
CAGAGCAGGATCCCCGGTCG

Downstream 100 bases:

>100_bases
ACGCATCATGAGCGGTCGCCCAGAACAATTGTTCCCGCTTTTCGCGGAACTTGAGACCTTGCAAGGCGTCGGACCAAAGA
TTGCCGAGCATTTTGCGCAG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MDEADARAEFEALQTKIEKADHDYHQKDAPTLSDADYDRLKRRYLALADAFPILAKDGTRVASVGAPAASGFGKVTHAQR
MMSLGNAFEDQDVEDFAVGLRRYLGLSSEAPLAFTAEPKIDGLSLSLRYEAGKLVQAATRGDGAVGENVTENARTISDIP
QEISGAPEVLEVRGEVYMSHADFEALNARHAETGGKIFANPRNAAAGSLRQLDAEITRARPLRFFAYSWGELSEPLAETQ
IDAIERLASLGFQTNPLTRCCEQISELLAHYHAIEEQRADLGYDIDGVVYKVNDLSLQERLGFRSTTPRWAIAHKFPAEL
AWTHLEGIDIQVGRTGALSPVARLHPVTVGGVVVSNATLHNEDYIAGLDSKGAPIRGGKDIRVGDWVQIYRAGDVIPKVA
DVDLSRRPEGTERYAFPTRCPRCDSPAVREEGDAVRRCSGGLICPAQAVEKLKHFVSRAAFDIEGLGAKQVEQFHSDGWV
KEPADIFELQQRYGSGLQQLKNREGWGEKSASALFAAIEDKRRIEFARLIFGLGIRHVGEVAAKDLALHFRTWSALAEAA
DLARAAALAHRAADEAEIVERQEAQASARRAKISEARNAAVATCAVAPDSQAAWDDLISVDGLGPTVALSLSDAFANPEE
RAAFDRLIAHLEIIEPDAPADDSPVAGKTVVFTGTLEKMTRAEAKARAEALGAKVSGSVSKKTDILVAGPGAGSKAAKAA
ELGIQTLDEDGWLDLIGQA

Sequences:

>Translated_739_residues
MDEADARAEFEALQTKIEKADHDYHQKDAPTLSDADYDRLKRRYLALADAFPILAKDGTRVASVGAPAASGFGKVTHAQR
MMSLGNAFEDQDVEDFAVGLRRYLGLSSEAPLAFTAEPKIDGLSLSLRYEAGKLVQAATRGDGAVGENVTENARTISDIP
QEISGAPEVLEVRGEVYMSHADFEALNARHAETGGKIFANPRNAAAGSLRQLDAEITRARPLRFFAYSWGELSEPLAETQ
IDAIERLASLGFQTNPLTRCCEQISELLAHYHAIEEQRADLGYDIDGVVYKVNDLSLQERLGFRSTTPRWAIAHKFPAEL
AWTHLEGIDIQVGRTGALSPVARLHPVTVGGVVVSNATLHNEDYIAGLDSKGAPIRGGKDIRVGDWVQIYRAGDVIPKVA
DVDLSRRPEGTERYAFPTRCPRCDSPAVREEGDAVRRCSGGLICPAQAVEKLKHFVSRAAFDIEGLGAKQVEQFHSDGWV
KEPADIFELQQRYGSGLQQLKNREGWGEKSASALFAAIEDKRRIEFARLIFGLGIRHVGEVAAKDLALHFRTWSALAEAA
DLARAAALAHRAADEAEIVERQEAQASARRAKISEARNAAVATCAVAPDSQAAWDDLISVDGLGPTVALSLSDAFANPEE
RAAFDRLIAHLEIIEPDAPADDSPVAGKTVVFTGTLEKMTRAEAKARAEALGAKVSGSVSKKTDILVAGPGAGSKAAKAA
ELGIQTLDEDGWLDLIGQA
>Mature_739_residues
MDEADARAEFEALQTKIEKADHDYHQKDAPTLSDADYDRLKRRYLALADAFPILAKDGTRVASVGAPAASGFGKVTHAQR
MMSLGNAFEDQDVEDFAVGLRRYLGLSSEAPLAFTAEPKIDGLSLSLRYEAGKLVQAATRGDGAVGENVTENARTISDIP
QEISGAPEVLEVRGEVYMSHADFEALNARHAETGGKIFANPRNAAAGSLRQLDAEITRARPLRFFAYSWGELSEPLAETQ
IDAIERLASLGFQTNPLTRCCEQISELLAHYHAIEEQRADLGYDIDGVVYKVNDLSLQERLGFRSTTPRWAIAHKFPAEL
AWTHLEGIDIQVGRTGALSPVARLHPVTVGGVVVSNATLHNEDYIAGLDSKGAPIRGGKDIRVGDWVQIYRAGDVIPKVA
DVDLSRRPEGTERYAFPTRCPRCDSPAVREEGDAVRRCSGGLICPAQAVEKLKHFVSRAAFDIEGLGAKQVEQFHSDGWV
KEPADIFELQQRYGSGLQQLKNREGWGEKSASALFAAIEDKRRIEFARLIFGLGIRHVGEVAAKDLALHFRTWSALAEAA
DLARAAALAHRAADEAEIVERQEAQASARRAKISEARNAAVATCAVAPDSQAAWDDLISVDGLGPTVALSLSDAFANPEE
RAAFDRLIAHLEIIEPDAPADDSPVAGKTVVFTGTLEKMTRAEAKARAEALGAKVSGSVSKKTDILVAGPGAGSKAAKAA
ELGIQTLDEDGWLDLIGQA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=736, Percent_Identity=43.3423913043478, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI87082305, Length=541, Percent_Identity=24.0295748613678, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SILST (Q1GGT5)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613393.1
- ProteinModelPortal:   Q1GGT5
- STRING:   Q1GGT5
- GeneID:   4075891
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1398
- NMPDR:   fig|292414.1.peg.3497
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q1GGT5
- ProtClustDB:   CLSK933607
- BioCyc:   SSP292414:TM1040_1398-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 79737; Mature: 79737

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 119-119 BINDING 117-117 BINDING 140-140 BINDING 175-175 BINDING 291-291 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEADARAEFEALQTKIEKADHDYHQKDAPTLSDADYDRLKRRYLALADAFPILAKDGTR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCE
VASVGAPAASGFGKVTHAQRMMSLGNAFEDQDVEDFAVGLRRYLGLSSEAPLAFTAEPKI
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCC
DGLSLSLRYEAGKLVQAATRGDGAVGENVTENARTISDIPQEISGAPEVLEVRGEVYMSH
CCEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHEEEC
ADFEALNARHAETGGKIFANPRNAAAGSLRQLDAEITRARPLRFFAYSWGELSEPLAETQ
CCHHHHCCHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
IDAIERLASLGFQTNPLTRCCEQISELLAHYHAIEEQRADLGYDIDGVVYKVNDLSLQER
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
LGFRSTTPRWAIAHKFPAELAWTHLEGIDIQVGRTGALSPVARLHPVTVGGVVVSNATLH
CCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCCCCHHHHCCEEECCEEEECCEEC
NEDYIAGLDSKGAPIRGGKDIRVGDWVQIYRAGDVIPKVADVDLSRRPEGTERYAFPTRC
CCCCEECCCCCCCCCCCCCCCCCCCHHEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCC
PRCDSPAVREEGDAVRRCSGGLICPAQAVEKLKHFVSRAAFDIEGLGAKQVEQFHSDGWV
CCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
KEPADIFELQQRYGSGLQQLKNREGWGEKSASALFAAIEDKRRIEFARLIFGLGIRHVGE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAKDLALHFRTWSALAEAADLARAAALAHRAADEAEIVERQEAQASARRAKISEARNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VATCAVAPDSQAAWDDLISVDGLGPTVALSLSDAFANPEERAAFDRLIAHLEIIEPDAPA
EEEEEECCCCCHHHHHHHCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHEECCCCCC
DDSPVAGKTVVFTGTLEKMTRAEAKARAEALGAKVSGSVSKKTDILVAGPGAGSKAAKAA
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHH
ELGIQTLDEDGWLDLIGQA
HHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MDEADARAEFEALQTKIEKADHDYHQKDAPTLSDADYDRLKRRYLALADAFPILAKDGTR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCE
VASVGAPAASGFGKVTHAQRMMSLGNAFEDQDVEDFAVGLRRYLGLSSEAPLAFTAEPKI
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCC
DGLSLSLRYEAGKLVQAATRGDGAVGENVTENARTISDIPQEISGAPEVLEVRGEVYMSH
CCEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHEEEC
ADFEALNARHAETGGKIFANPRNAAAGSLRQLDAEITRARPLRFFAYSWGELSEPLAETQ
CCHHHHCCHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
IDAIERLASLGFQTNPLTRCCEQISELLAHYHAIEEQRADLGYDIDGVVYKVNDLSLQER
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
LGFRSTTPRWAIAHKFPAELAWTHLEGIDIQVGRTGALSPVARLHPVTVGGVVVSNATLH
CCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCCCCHHHHCCEEECCEEEECCEEC
NEDYIAGLDSKGAPIRGGKDIRVGDWVQIYRAGDVIPKVADVDLSRRPEGTERYAFPTRC
CCCCEECCCCCCCCCCCCCCCCCCCHHEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCC
PRCDSPAVREEGDAVRRCSGGLICPAQAVEKLKHFVSRAAFDIEGLGAKQVEQFHSDGWV
CCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
KEPADIFELQQRYGSGLQQLKNREGWGEKSASALFAAIEDKRRIEFARLIFGLGIRHVGE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAKDLALHFRTWSALAEAADLARAAALAHRAADEAEIVERQEAQASARRAKISEARNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VATCAVAPDSQAAWDDLISVDGLGPTVALSLSDAFANPEERAAFDRLIAHLEIIEPDAPA
EEEEEECCCCCHHHHHHHCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHEECCCCCC
DDSPVAGKTVVFTGTLEKMTRAEAKARAEALGAKVSGSVSKKTDILVAGPGAGSKAAKAA
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHH
ELGIQTLDEDGWLDLIGQA
HHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA