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The map label for this gene is guaA

Identifier: 99081216

GI number: 99081216

Start: 1468257

End: 1469819

Strand: Direct

Name: guaA

Synonym: TM1040_1375

Alternate gene names: 99081216

Gene position: 1468257-1469819 (Clockwise)

Preceding gene: 99081214

Following gene: 99081217

Centisome position: 45.87

GC content: 59.25

Gene sequence:

>1563_bases
ATGACCGAGACAGCCCATGACCGCCTTTTAATTATAGACTTCGGCAGCCAGGTAACGCAGCTGATTGCGCGCCGCCTGCG
CGAGTTGAACGTCTATTGTGAAATCCACCCCTATCAGAATGTCACCATGGACTTCGTGCGCGAGTTCGCGCCCAAGGCGG
TGATCTTCTCTGGTGGTCCCGACAGCGTGACGCGCGAAGGCTCTCCCCGCGCGCCGCAAGAGATTTTTGACTACGGCGTG
CCGATCCTTGGCATCTGTTATGGCCAGCAAGTGATGATGCATCAGCTTGGCGGCACTGTTCAATCCGGCCATGGAACCGC
CGAATTTGGCCGCGCCTATGTGACACCCACCGAAGAGCGCATCGACATGCTCAGCGGCTGGTTCCTGGATCAGACGGAAC
AGGTCTGGATGAGCCACGGCGACCATGTCTCTGAAATCGCACCCGGTTTCAAGGTCTACGGCACCTCGCCCAACGCGCCA
TTTGCGATCACGGCGGATCTGGAGCGCAACTTCTACGCTGTTCAGTTCCACCCCGAGGTTCATCACACTCCCAACGGCAA
GACGCTCTATGAAAACTTCGTGCGTCTGGCCGGGTTCAGCGGTGACTGGACCATGGGCGCCTATCGCGAGCAGATGGTCG
AAACCATCCGCGAGCAGGTCGGCGACAAGAAAGTCATCTGTGCCCTTTCGGGTGGCGTCGACAGTTCCGTTGCGGCGGCT
CTGATCCACGAGGCGATCGGCGATCAGCTGACCTGTGTGTTTGTGGACCATGGTCTTCTGCGCAAGAACGAGGCGGAAGA
AGTCGTCGGCATGTTCCGCGACCACATGAACCTGCAGGTCATCCACGCGGATGAGACCGAGCTCTTTCTCGGTGAGCTAG
AAGGTCAGTCCGACCCCGAAACCAAGCGCAAGATTATCGGCAAGCTGTTCATCGACGTGTTCCAGAAATACGCCGATCAG
ATCGAAGGCGCGGAGTTCCTGGCCCAAGGTACGCTCTACCCGGATGTCATCGAGTCGGTCTCGTTCTCTGGTGGCCCTTC
GGTGACGATCAAGTCGCACCACAACGTCGGTGGTCTGCCCGAAAAAATGGGCCTGAAACTGGTGGAGCCGCTGCGCGAGC
TCTTCAAGGACGAAGTGCGCGCGCTCGGGCGTGAACTTGGCCTGCCCGACAGCTTTATTGGACGGCACCCCTTCCCCGGA
CCGGGTCTGGCGATCCGTTGCCCCGGCGAGATCACCCGCGACAAGCTGGACATCCTGCGCGAAGCGGATGCCATCTATAT
CGACCAGATCCGCAAACACGGTCTTTATGATGAGATCTGGCAGGCCTTTGTGGCGATCCTGCCGGTGCGCACCGTGGGCG
TGATGGGCGACGGTCGCACCTATGACTACGCCTGTGCCCTGCGCGCGGTCACCTCGGTCGATGGGATGACGGCGGATTAC
TACCCGTTCAGCCATGAGTTCCTTGGTGAGACCGCAACGCGGATCATCAATGAAGTCAAAGGCATCAACCGTTGCACCTA
TGACATCACCTCAAAGCCTCCGGGCACGATCGAGTGGGAATGA

Upstream 100 bases:

>100_bases
ACATTCCGGAGTAGAATTTCCGGACTCGGAGAGAGAATTTGCCCCGCCACAGCCCATCTGACCTCTTGCCAGACGTCCCG
CCAGACCCTAAGAGCCAAGC

Downstream 100 bases:

>100_bases
TCTCAGGCCCAAGTCTGATACGAGCCCCGGTGCTGCGCACCGGGGCTTTTCATTTCTGACCCCCACGGGCTCCGCCTCAA
AAGACCGAGGAGCGCGAAGC

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTETAHDRLLIIDFGSQVTQLIARRLRELNVYCEIHPYQNVTMDFVREFAPKAVIFSGGPDSVTREGSPRAPQEIFDYGV
PILGICYGQQVMMHQLGGTVQSGHGTAEFGRAYVTPTEERIDMLSGWFLDQTEQVWMSHGDHVSEIAPGFKVYGTSPNAP
FAITADLERNFYAVQFHPEVHHTPNGKTLYENFVRLAGFSGDWTMGAYREQMVETIREQVGDKKVICALSGGVDSSVAAA
LIHEAIGDQLTCVFVDHGLLRKNEAEEVVGMFRDHMNLQVIHADETELFLGELEGQSDPETKRKIIGKLFIDVFQKYADQ
IEGAEFLAQGTLYPDVIESVSFSGGPSVTIKSHHNVGGLPEKMGLKLVEPLRELFKDEVRALGRELGLPDSFIGRHPFPG
PGLAIRCPGEITRDKLDILREADAIYIDQIRKHGLYDEIWQAFVAILPVRTVGVMGDGRTYDYACALRAVTSVDGMTADY
YPFSHEFLGETATRIINEVKGINRCTYDITSKPPGTIEWE

Sequences:

>Translated_520_residues
MTETAHDRLLIIDFGSQVTQLIARRLRELNVYCEIHPYQNVTMDFVREFAPKAVIFSGGPDSVTREGSPRAPQEIFDYGV
PILGICYGQQVMMHQLGGTVQSGHGTAEFGRAYVTPTEERIDMLSGWFLDQTEQVWMSHGDHVSEIAPGFKVYGTSPNAP
FAITADLERNFYAVQFHPEVHHTPNGKTLYENFVRLAGFSGDWTMGAYREQMVETIREQVGDKKVICALSGGVDSSVAAA
LIHEAIGDQLTCVFVDHGLLRKNEAEEVVGMFRDHMNLQVIHADETELFLGELEGQSDPETKRKIIGKLFIDVFQKYADQ
IEGAEFLAQGTLYPDVIESVSFSGGPSVTIKSHHNVGGLPEKMGLKLVEPLRELFKDEVRALGRELGLPDSFIGRHPFPG
PGLAIRCPGEITRDKLDILREADAIYIDQIRKHGLYDEIWQAFVAILPVRTVGVMGDGRTYDYACALRAVTSVDGMTADY
YPFSHEFLGETATRIINEVKGINRCTYDITSKPPGTIEWE
>Mature_519_residues
TETAHDRLLIIDFGSQVTQLIARRLRELNVYCEIHPYQNVTMDFVREFAPKAVIFSGGPDSVTREGSPRAPQEIFDYGVP
ILGICYGQQVMMHQLGGTVQSGHGTAEFGRAYVTPTEERIDMLSGWFLDQTEQVWMSHGDHVSEIAPGFKVYGTSPNAPF
AITADLERNFYAVQFHPEVHHTPNGKTLYENFVRLAGFSGDWTMGAYREQMVETIREQVGDKKVICALSGGVDSSVAAAL
IHEAIGDQLTCVFVDHGLLRKNEAEEVVGMFRDHMNLQVIHADETELFLGELEGQSDPETKRKIIGKLFIDVFQKYADQI
EGAEFLAQGTLYPDVIESVSFSGGPSVTIKSHHNVGGLPEKMGLKLVEPLRELFKDEVRALGRELGLPDSFIGRHPFPGP
GLAIRCPGEITRDKLDILREADAIYIDQIRKHGLYDEIWQAFVAILPVRTVGVMGDGRTYDYACALRAVTSVDGMTADYY
PFSHEFLGETATRIINEVKGINRCTYDITSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=521, Percent_Identity=37.4280230326296, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1788854, Length=531, Percent_Identity=53.4839924670433, Blast_Score=560, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71992710, Length=578, Percent_Identity=36.6782006920415, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI133901714, Length=578, Percent_Identity=36.6782006920415, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI71992717, Length=578, Percent_Identity=36.6782006920415, Blast_Score=299, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6323873, Length=521, Percent_Identity=50.8637236084453, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI281365319, Length=552, Percent_Identity=34.963768115942, Blast_Score=269, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_SILST (Q1GGV8)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613370.1
- ProteinModelPortal:   Q1GGV8
- SMR:   Q1GGV8
- STRING:   Q1GGV8
- GeneID:   4075868
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1375
- NMPDR:   fig|292414.1.peg.3518
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- PhylomeDB:   Q1GGV8
- ProtClustDB:   PRK00074
- BioCyc:   SSP292414:TM1040_1375-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001962
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C

EC number: =6.3.5.2

Molecular weight: Translated: 57952; Mature: 57821

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 86-86 ACT_SITE 177-177 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETAHDRLLIIDFGSQVTQLIARRLRELNVYCEIHPYQNVTMDFVREFAPKAVIFSGGP
CCCCCCCEEEEEECCHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHCCCEEEECCCC
DSVTREGSPRAPQEIFDYGVPILGICYGQQVMMHQLGGTVQSGHGTAEFGRAYVTPTEER
CCCCCCCCCCCCHHHHHCCCCCEEHHHHHHHHHHHHCCCEECCCCCHHHCCEECCCCHHH
IDMLSGWFLDQTEQVWMSHGDHVSEIAPGFKVYGTSPNAPFAITADLERNFYAVQFHPEV
HHHHHCCCCCHHHHHHHHCCCCHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEECCCC
HHTPNGKTLYENFVRLAGFSGDWTMGAYREQMVETIREQVGDKKVICALSGGVDSSVAAA
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
LIHEAIGDQLTCVFVDHGLLRKNEAEEVVGMFRDHMNLQVIHADETELFLGELEGQSDPE
HHHHHHCCCEEEEEECCCHHCCCHHHHHHHHHHHCCCEEEEECCCCEEEHEECCCCCCCH
TKRKIIGKLFIDVFQKYADQIEGAEFLAQGTLYPDVIESVSFSGGPSVTIKSHHNVGGLP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCC
EKMGLKLVEPLRELFKDEVRALGRELGLPDSFIGRHPFPGPGLAIRCPGEITRDKLDILR
HHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEECCCHHHHHHHHHHH
EADAIYIDQIRKHGLYDEIWQAFVAILPVRTVGVMGDGRTYDYACALRAVTSVDGMTADY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCCC
YPFSHEFLGETATRIINEVKGINRCTYDITSKPPGTIEWE
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TETAHDRLLIIDFGSQVTQLIARRLRELNVYCEIHPYQNVTMDFVREFAPKAVIFSGGP
CCCCCCEEEEEECCHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHCCCEEEECCCC
DSVTREGSPRAPQEIFDYGVPILGICYGQQVMMHQLGGTVQSGHGTAEFGRAYVTPTEER
CCCCCCCCCCCCHHHHHCCCCCEEHHHHHHHHHHHHCCCEECCCCCHHHCCEECCCCHHH
IDMLSGWFLDQTEQVWMSHGDHVSEIAPGFKVYGTSPNAPFAITADLERNFYAVQFHPEV
HHHHHCCCCCHHHHHHHHCCCCHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEECCCC
HHTPNGKTLYENFVRLAGFSGDWTMGAYREQMVETIREQVGDKKVICALSGGVDSSVAAA
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
LIHEAIGDQLTCVFVDHGLLRKNEAEEVVGMFRDHMNLQVIHADETELFLGELEGQSDPE
HHHHHHCCCEEEEEECCCHHCCCHHHHHHHHHHHCCCEEEEECCCCEEEHEECCCCCCCH
TKRKIIGKLFIDVFQKYADQIEGAEFLAQGTLYPDVIESVSFSGGPSVTIKSHHNVGGLP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCC
EKMGLKLVEPLRELFKDEVRALGRELGLPDSFIGRHPFPGPGLAIRCPGEITRDKLDILR
HHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEECCCHHHHHHHHHHH
EADAIYIDQIRKHGLYDEIWQAFVAILPVRTVGVMGDGRTYDYACALRAVTSVDGMTADY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCCC
YPFSHEFLGETATRIINEVKGINRCTYDITSKPPGTIEWE
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA