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The map label for this gene is psuK [H]

Identifier: 99081179

GI number: 99081179

Start: 1425754

End: 1426662

Strand: Direct

Name: psuK [H]

Synonym: TM1040_1338

Alternate gene names: 99081179

Gene position: 1425754-1426662 (Clockwise)

Preceding gene: 99081178

Following gene: 99081180

Centisome position: 44.54

GC content: 63.92

Gene sequence:

>909_bases
ATGACTGAAAGCGCTGATTTCTCCAAACCTGATATTCTCTGTGTCGGTTCCGTCCTTTGGGACATTATCGGCAGAAGCAC
CATGGAGATGCAGCGTGGCTCTGACATGCCCGGGCGCATCACCCGCCTGCCCGGCGGAGTTGCCATGAACATCGCGATGA
CGCTGGCGCGTTTTGGAATGAAACCCGCCGTGCTGAGCGCCATTGGGCGCGATCCCGAAGGGGATGAGCTGGTTTCGGCC
TGTGGGCATCTGGGGCTGATGACGGACTATCTATATCGCTCGGAGGATTTGCCGACCGATCGCTACATGGCGGTTGAGGG
TGCCAATGGGCTGATCGCGGCGATTGCCGATGCCCATTCGCTCGAGGCAGCAGGCGACAAGATCCTGCGCCCCCTCGTCG
AGGGGCCGCTGGGCACGGCCGATGCGCCGTTTGAAGGACCGGTAGCGCTTGATGGGAACCTGAAGCTGGCGCTTCTGGAT
GAGATCGCGACCCATCCTGCCTTTGCGCGCTCGGATTTGCGCGTCGCGCCCGCCTCGCCCGGCAAGGCGGAACGGCTGCG
CCCGTTTCTGACCCATGCCCGCGGCACGCTTTACGTGAACCTCGAAGAGGCAGGCCTGCTGTGTCAGGACCAGTTTACCG
ACAGTGAAACCGCTGCACAGGCGCTGCTGGCGCGCGGGGCTGCCCGCGTTCTGGTTACCGATGGTGGCCAATCCGCCACC
GAAGCCGATGCCAGCGGTCACCGCACCCAAGTTCCGCCACGCGTCACCGTCGCCCGGGTCACCGGCGCAGGCGATACGTT
CATGGCCGCCCATATCGCTGCTGAGGCGCGGGGAGAAGACCGCGACAGCGCGCTCGCTTCTGCGCTTCATGCAGCCGCAA
CCTATGTTTCCGGAGAACCGCCCCTTTGA

Upstream 100 bases:

>100_bases
GGCATCGCCCGGCCGTGCACAGTCATTCCTGTTGCGACAAGAGGCTTCCGGCACCGTAAATGGGCTTGCGGGGGCCAACG
AAAGGCGTCATGAGATCCCT

Downstream 100 bases:

>100_bases
TCGACATTACCTATTCCGCAGAAGTCGAAGCAGCCCGCGCCGCAGGCAAACCAATTGTTGCGCTTGAAAGCACCATCATT
ACCCACGGTATGCCTTACCC

Product: PfkB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTESADFSKPDILCVGSVLWDIIGRSTMEMQRGSDMPGRITRLPGGVAMNIAMTLARFGMKPAVLSAIGRDPEGDELVSA
CGHLGLMTDYLYRSEDLPTDRYMAVEGANGLIAAIADAHSLEAAGDKILRPLVEGPLGTADAPFEGPVALDGNLKLALLD
EIATHPAFARSDLRVAPASPGKAERLRPFLTHARGTLYVNLEEAGLLCQDQFTDSETAAQALLARGAARVLVTDGGQSAT
EADASGHRTQVPPRVTVARVTGAGDTFMAAHIAAEARGEDRDSALASALHAAATYVSGEPPL

Sequences:

>Translated_302_residues
MTESADFSKPDILCVGSVLWDIIGRSTMEMQRGSDMPGRITRLPGGVAMNIAMTLARFGMKPAVLSAIGRDPEGDELVSA
CGHLGLMTDYLYRSEDLPTDRYMAVEGANGLIAAIADAHSLEAAGDKILRPLVEGPLGTADAPFEGPVALDGNLKLALLD
EIATHPAFARSDLRVAPASPGKAERLRPFLTHARGTLYVNLEEAGLLCQDQFTDSETAAQALLARGAARVLVTDGGQSAT
EADASGHRTQVPPRVTVARVTGAGDTFMAAHIAAEARGEDRDSALASALHAAATYVSGEPPL
>Mature_301_residues
TESADFSKPDILCVGSVLWDIIGRSTMEMQRGSDMPGRITRLPGGVAMNIAMTLARFGMKPAVLSAIGRDPEGDELVSAC
GHLGLMTDYLYRSEDLPTDRYMAVEGANGLIAAIADAHSLEAAGDKILRPLVEGPLGTADAPFEGPVALDGNLKLALLDE
IATHPAFARSDLRVAPASPGKAERLRPFLTHARGTLYVNLEEAGLLCQDQFTDSETAAQALLARGAARVLVTDGGQSATE
ADASGHRTQVPPRVTVARVTGAGDTFMAAHIAAEARGEDRDSALASALHAAATYVSGEPPL

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=278, Percent_Identity=29.136690647482, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788484, Length=301, Percent_Identity=29.5681063122924, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 31585; Mature: 31454

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESADFSKPDILCVGSVLWDIIGRSTMEMQRGSDMPGRITRLPGGVAMNIAMTLARFGM
CCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCC
KPAVLSAIGRDPEGDELVSACGHLGLMTDYLYRSEDLPTDRYMAVEGANGLIAAIADAHS
CHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCHHH
LEAAGDKILRPLVEGPLGTADAPFEGPVALDGNLKLALLDEIATHPAFARSDLRVAPASP
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEHHHHHCCCCHHCCCCEECCCCC
GKAERLRPFLTHARGTLYVNLEEAGLLCQDQFTDSETAAQALLARGAARVLVTDGGQSAT
CHHHHHHHHHHCCCCEEEEEEHHCCCEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
EADASGHRTQVPPRVTVARVTGAGDTFMAAHIAAEARGEDRDSALASALHAAATYVSGEP
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
PL
CC
>Mature Secondary Structure 
TESADFSKPDILCVGSVLWDIIGRSTMEMQRGSDMPGRITRLPGGVAMNIAMTLARFGM
CCCCCCCCCCEEEHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCC
KPAVLSAIGRDPEGDELVSACGHLGLMTDYLYRSEDLPTDRYMAVEGANGLIAAIADAHS
CHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCHHH
LEAAGDKILRPLVEGPLGTADAPFEGPVALDGNLKLALLDEIATHPAFARSDLRVAPASP
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEHHHHHCCCCHHCCCCEECCCCC
GKAERLRPFLTHARGTLYVNLEEAGLLCQDQFTDSETAAQALLARGAARVLVTDGGQSAT
CHHHHHHHHHHCCCCEEEEEEHHCCCEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
EADASGHRTQVPPRVTVARVTGAGDTFMAAHIAAEARGEDRDSALASALHAAATYVSGEP
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
PL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]