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The map label for this gene is rimO

Identifier: 99080490

GI number: 99080490

Start: 693219

End: 694622

Strand: Reverse

Name: rimO

Synonym: TM1040_0649

Alternate gene names: 99080490

Gene position: 694622-693219 (Counterclockwise)

Preceding gene: 99080493

Following gene: 99080489

Centisome position: 21.7

GC content: 62.46

Gene sequence:

>1404_bases
ATGACCAGCAACCCGCCAGATCTCCGCCCCGACCTCGCCCCCAAACCCACCTTTGGCACGGCGCCCCGTCCCGACCAGCC
GACCCTCGGCATGGTCTCCTTGGGCTGCCCAAAGGCCCTGGTGGACAGCGAACGTATCCTCACCCGCTTGCGCGCCGAAG
GATATGGGATCTCGGCGGATTATGCCGGCGCGGATGCGGTGATCGTCAACACCTGCGGCTTTCTCGACAGCGCCAAGGCC
GAGAGCCTCGAGGCGATTGGCGAAGCGCTGAAGGAAAACGGCAAGGTGATCGTCACAGGGTGCCTTGGTGCAGAGCCGGA
CTATATCCGCGAGCACCATCCGCGCATCCTCGCCGTCACGGGCCCGCATCAATACGAGCAGGTGCTGGATGCGGTCCATG
CGGCGGTGCCGCCCAGCCCCGACCCCTTTGTGGACCTTCTGCCCGCCACGGGTGTGAGCCTCACGCCCCGGCATTTCAGC
TATCTGAAGATCTCTGAGGGCTGCAACCACAAGTGCAAGTTCTGCATTATCCCCGACATGCGCGGCAAACTTGCGTCGCG
CCCGGCCCATGCGGTTTTACGCGAGGCCGAGAAACTGGTCACCGCTGGCGTCCGAGAGCTTCTGGTCATCTCGCAGGATA
CCTCGGCCTATGGGCTCGATCGAAAATATGACGTGAACCCGTGGAAAGACGGCGAGGTTCGAAGCCATATCACCGATCTG
AGCCGCGCATTGGGGGAGCTGGCCCCCGCCGATCAGCTCTGGGTGCGGCTCCATTACGTCTACCCCTACCCGCATGTACG
CGAGTTGATCCCGCTGATGGCCGACCCTGAAAATGCTGTGCTGCCCTATCTCGACATTCCGTTCCAGCACGCCCACCCGG
ATGTGCTCCGTCGCATGGCCCGCCCGGCAGCGGCAGCAAAGACGCTGGACGAAATCCGCGCCTGGCGCGCCATCTGTCCT
GACATCACCCTGCGGTCGACCTTCATCGTCGGCTATCCAGGCGAAACAGAGGCCGAATTCCAGCACTTGCTCGACTGGAT
GGATGAGGCTCAACTGGATCGCGTGGGCTGCTTCAAATATGAAAACGTCGACGGCGCCCGCTCCAATGACTTGCCCGACC
ACGTCGCGGAGGAGGTCAAGCAAAACCGCTGGGAGCGCTTCATGGAAAAAGCACAGGCCATTTCCGAGGCCAAACTCGCA
TCCAAAGTGGGCCAGACCCTGCAGGTGATCGTGGACGAGATCGACGAGGACGGGATCGCCACCTGCCGCACCAAGGCGGA
TGCTCCGGAAATCGACGGCAACCTCTTTATCGACGAAGGCACCGCAAATCTGAGCGTCGGGGATCTGGTCACGGTCGAAG
TGGATGAAGCCGGCGAGTATGACCTTTGGGGGGCGCTGCGCTAA

Upstream 100 bases:

>100_bases
CAACGCCGCGGCCAAAAACCGCTCGCGCAAGCCCGCGCCTGTGAGCGGATCCCGGAAATCCTCCATTCCCAAGCGCCGCG
CTGCGGTTTATAGGACGGCA

Downstream 100 bases:

>100_bases
GGCGGCACACCCAATCCGGCCACATTCAGGACGGAGCAGGTTTGAGAGTTCACCTTGACCTGCTCCTCTCCGCCCTCAAT
CTTGCAGTCGACAGCAGGAG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTSNPPDLRPDLAPKPTFGTAPRPDQPTLGMVSLGCPKALVDSERILTRLRAEGYGISADYAGADAVIVNTCGFLDSAKA
ESLEAIGEALKENGKVIVTGCLGAEPDYIREHHPRILAVTGPHQYEQVLDAVHAAVPPSPDPFVDLLPATGVSLTPRHFS
YLKISEGCNHKCKFCIIPDMRGKLASRPAHAVLREAEKLVTAGVRELLVISQDTSAYGLDRKYDVNPWKDGEVRSHITDL
SRALGELAPADQLWVRLHYVYPYPHVRELIPLMADPENAVLPYLDIPFQHAHPDVLRRMARPAAAAKTLDEIRAWRAICP
DITLRSTFIVGYPGETEAEFQHLLDWMDEAQLDRVGCFKYENVDGARSNDLPDHVAEEVKQNRWERFMEKAQAISEAKLA
SKVGQTLQVIVDEIDEDGIATCRTKADAPEIDGNLFIDEGTANLSVGDLVTVEVDEAGEYDLWGALR

Sequences:

>Translated_467_residues
MTSNPPDLRPDLAPKPTFGTAPRPDQPTLGMVSLGCPKALVDSERILTRLRAEGYGISADYAGADAVIVNTCGFLDSAKA
ESLEAIGEALKENGKVIVTGCLGAEPDYIREHHPRILAVTGPHQYEQVLDAVHAAVPPSPDPFVDLLPATGVSLTPRHFS
YLKISEGCNHKCKFCIIPDMRGKLASRPAHAVLREAEKLVTAGVRELLVISQDTSAYGLDRKYDVNPWKDGEVRSHITDL
SRALGELAPADQLWVRLHYVYPYPHVRELIPLMADPENAVLPYLDIPFQHAHPDVLRRMARPAAAAKTLDEIRAWRAICP
DITLRSTFIVGYPGETEAEFQHLLDWMDEAQLDRVGCFKYENVDGARSNDLPDHVAEEVKQNRWERFMEKAQAISEAKLA
SKVGQTLQVIVDEIDEDGIATCRTKADAPEIDGNLFIDEGTANLSVGDLVTVEVDEAGEYDLWGALR
>Mature_466_residues
TSNPPDLRPDLAPKPTFGTAPRPDQPTLGMVSLGCPKALVDSERILTRLRAEGYGISADYAGADAVIVNTCGFLDSAKAE
SLEAIGEALKENGKVIVTGCLGAEPDYIREHHPRILAVTGPHQYEQVLDAVHAAVPPSPDPFVDLLPATGVSLTPRHFSY
LKISEGCNHKCKFCIIPDMRGKLASRPAHAVLREAEKLVTAGVRELLVISQDTSAYGLDRKYDVNPWKDGEVRSHITDLS
RALGELAPADQLWVRLHYVYPYPHVRELIPLMADPENAVLPYLDIPFQHAHPDVLRRMARPAAAAKTLDEIRAWRAICPD
ITLRSTFIVGYPGETEAEFQHLLDWMDEAQLDRVGCFKYENVDGARSNDLPDHVAEEVKQNRWERFMEKAQAISEAKLAS
KVGQTLQVIVDEIDEDGIATCRTKADAPEIDGNLFIDEGTANLSVGDLVTVEVDEAGEYDLWGALR

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=444, Percent_Identity=24.3243243243243, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28872782, Length=500, Percent_Identity=24.6, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI28872784, Length=500, Percent_Identity=25, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1787057, Length=442, Percent_Identity=60.8597285067873, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1786882, Length=456, Percent_Identity=26.0964912280702, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=412, Percent_Identity=24.7572815533981, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356207, Length=484, Percent_Identity=24.1735537190083, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922432, Length=424, Percent_Identity=23.8207547169811, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_SILST (Q1GIY4)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612644.1
- ProteinModelPortal:   Q1GIY4
- SMR:   Q1GIY4
- STRING:   Q1GIY4
- GeneID:   4078162
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0649
- NMPDR:   fig|292414.1.peg.2757
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q1GIY4
- ProtClustDB:   PRK14862
- BioCyc:   SSP292414:TM1040_0649-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51451; Mature: 51320

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNPPDLRPDLAPKPTFGTAPRPDQPTLGMVSLGCPKALVDSERILTRLRAEGYGISAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
YAGADAVIVNTCGFLDSAKAESLEAIGEALKENGKVIVTGCLGAEPDYIREHHPRILAVT
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCEEEEEE
GPHQYEQVLDAVHAAVPPSPDPFVDLLPATGVSLTPRHFSYLKISEGCNHKCKFCIIPDM
CCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEECCCCCEEEEECCCCCCCEEEEECCCC
RGKLASRPAHAVLREAEKLVTAGVRELLVISQDTSAYGLDRKYDVNPWKDGEVRSHITDL
CCHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRALGELAPADQLWVRLHYVYPYPHVRELIPLMADPENAVLPYLDIPFQHAHPDVLRRMA
HHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHH
RPAAAAKTLDEIRAWRAICPDITLRSTFIVGYPGETEAEFQHLLDWMDEAQLDRVGCFKY
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEE
ENVDGARSNDLPDHVAEEVKQNRWERFMEKAQAISEAKLASKVGQTLQVIVDEIDEDGIA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TCRTKADAPEIDGNLFIDEGTANLSVGDLVTVEVDEAGEYDLWGALR
EECCCCCCCCCCCCEEEECCCCCCEECCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TSNPPDLRPDLAPKPTFGTAPRPDQPTLGMVSLGCPKALVDSERILTRLRAEGYGISAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
YAGADAVIVNTCGFLDSAKAESLEAIGEALKENGKVIVTGCLGAEPDYIREHHPRILAVT
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCEEEEEE
GPHQYEQVLDAVHAAVPPSPDPFVDLLPATGVSLTPRHFSYLKISEGCNHKCKFCIIPDM
CCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEECCCCCEEEEECCCCCCCEEEEECCCC
RGKLASRPAHAVLREAEKLVTAGVRELLVISQDTSAYGLDRKYDVNPWKDGEVRSHITDL
CCHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRALGELAPADQLWVRLHYVYPYPHVRELIPLMADPENAVLPYLDIPFQHAHPDVLRRMA
HHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHH
RPAAAAKTLDEIRAWRAICPDITLRSTFIVGYPGETEAEFQHLLDWMDEAQLDRVGCFKY
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEE
ENVDGARSNDLPDHVAEEVKQNRWERFMEKAQAISEAKLASKVGQTLQVIVDEIDEDGIA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TCRTKADAPEIDGNLFIDEGTANLSVGDLVTVEVDEAGEYDLWGALR
EECCCCCCCCCCCCEEEECCCCCCEECCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA