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The map label for this gene is gyaR [H]

Identifier: 99080466

GI number: 99080466

Start: 662962

End: 663930

Strand: Direct

Name: gyaR [H]

Synonym: TM1040_0625

Alternate gene names: 99080466

Gene position: 662962-663930 (Clockwise)

Preceding gene: 99080465

Following gene: 99080468

Centisome position: 20.71

GC content: 63.26

Gene sequence:

>969_bases
ATGGCGGCTTCAGAACGTCGGCGCCTGTTGATCACGCGCCCCATGACCGCAGCGGTTGAGGCGCGCGCGCGCGCAGAACT
CGACGTGGAGATCCGTCAAGAGACCACGCCTCTGAGCCCAGAGGAAATGAGACAGAGCCTCGGGGCGTTTGATCTTGTGA
TGCCCACGCTCGGAGATGCCTATTCGGCGGATGTCTTTGCAGCGGTGCCACAGCCTCGGTGCCGTCTGCTCGCTAATTTT
GGGGTTGGCTTCAACCATATTGATGCTGAAGCGGCGCGGGCGGCCGGGGTGGAGGTCACCAATACCCCCGGTGCCGTTAC
GGATGCCACGGCCGACATTGCATTGACGCTGATGCTGATGACCGCGCGACGGGCTGGCGAGGGCGAGCGGCTGGTGCGCT
CGGGGCAGTGGCAGGGCTGGCACCCGACACAGATGCTCGGGCTGCATCTGTCGGGCAAGCGGCTTGGTGTGGTTGGGCTC
GGACGGATTGGCGATGCCATCGCGCGACGCGCACATTTCGGCTTTGGCATGGAGATTTCCTATCTCGCGCGCAGCGACAA
AGAAACTGGCTATCCTGCCACGCGGGCCTCGAACCTGATCGAGCTCGCCGCGTCGGTCGATATGCTTGTTGTGGCGGTGC
CCGGAGGTGCGGAAACCCGGCATCTGATCAATGCGGACGTGCTGGCAGCGCTGCCGTCTCATGCGCATCTGGTCAATATC
GCGCGCGGCGAGGTGGTCGATGAGGCCGCTTTGATTACCGCTTTGCAAGCGGGTCAGATCGCGGGCGCAGGGCTTGATGT
CTATGAGTTTGAGCCAAAGGTGCCAGCGGAGCTCAGAGCTATGGAGCAGGTGACGCTGCTGCCGCATTTGGGCACGGCCA
CCGAAGAGGTTCGCAGCGCCATGGGGCATATGGCGCTGGACAACTGCGTGGCATTTCTGACCGGGGCGCCTTTGCCAAAC
CCTGTGTGA

Upstream 100 bases:

>100_bases
TGCTTGTACTCGGCCTGCCGGATGAGAATGGTGAGATTGTTCTGATTGGCCCTGATGGCGATGTGTCCGCGCAGGTCCCG
CGCGGGGGGCGGATGCACTG

Downstream 100 bases:

>100_bases
TGGATGAACCAGGCGCGAGGGAGATCTCGTGAAGGCAGGGGCACTGCCCCTCTGGGCCCACAAGGGGCCCATTCACCCCG
GGATATTTGCGGTTAGAAGA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MAASERRRLLITRPMTAAVEARARAELDVEIRQETTPLSPEEMRQSLGAFDLVMPTLGDAYSADVFAAVPQPRCRLLANF
GVGFNHIDAEAARAAGVEVTNTPGAVTDATADIALTLMLMTARRAGEGERLVRSGQWQGWHPTQMLGLHLSGKRLGVVGL
GRIGDAIARRAHFGFGMEISYLARSDKETGYPATRASNLIELAASVDMLVVAVPGGAETRHLINADVLAALPSHAHLVNI
ARGEVVDEAALITALQAGQIAGAGLDVYEFEPKVPAELRAMEQVTLLPHLGTATEEVRSAMGHMALDNCVAFLTGAPLPN
PV

Sequences:

>Translated_322_residues
MAASERRRLLITRPMTAAVEARARAELDVEIRQETTPLSPEEMRQSLGAFDLVMPTLGDAYSADVFAAVPQPRCRLLANF
GVGFNHIDAEAARAAGVEVTNTPGAVTDATADIALTLMLMTARRAGEGERLVRSGQWQGWHPTQMLGLHLSGKRLGVVGL
GRIGDAIARRAHFGFGMEISYLARSDKETGYPATRASNLIELAASVDMLVVAVPGGAETRHLINADVLAALPSHAHLVNI
ARGEVVDEAALITALQAGQIAGAGLDVYEFEPKVPAELRAMEQVTLLPHLGTATEEVRSAMGHMALDNCVAFLTGAPLPN
PV
>Mature_321_residues
AASERRRLLITRPMTAAVEARARAELDVEIRQETTPLSPEEMRQSLGAFDLVMPTLGDAYSADVFAAVPQPRCRLLANFG
VGFNHIDAEAARAAGVEVTNTPGAVTDATADIALTLMLMTARRAGEGERLVRSGQWQGWHPTQMLGLHLSGKRLGVVGLG
RIGDAIARRAHFGFGMEISYLARSDKETGYPATRASNLIELAASVDMLVVAVPGGAETRHLINADVLAALPSHAHLVNIA
RGEVVDEAALITALQAGQIAGAGLDVYEFEPKVPAELRAMEQVTLLPHLGTATEEVRSAMGHMALDNCVAFLTGAPLPNP
V

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=320, Percent_Identity=32.1875, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI23308577, Length=281, Percent_Identity=32.7402135231317, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI61743967, Length=235, Percent_Identity=33.6170212765957, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI4557497, Length=235, Percent_Identity=33.6170212765957, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=31.8518518518519, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=31.8518518518519, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI145580575, Length=270, Percent_Identity=31.8518518518519, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI87082289, Length=294, Percent_Identity=37.0748299319728, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789279, Length=228, Percent_Identity=28.5087719298246, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787645, Length=258, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081824, Length=188, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=285, Percent_Identity=30.5263157894737, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=236, Percent_Identity=28.8135593220339, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324055, Length=246, Percent_Identity=38.2113821138211, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320925, Length=241, Percent_Identity=29.8755186721992, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=241, Percent_Identity=29.8755186721992, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321253, Length=171, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325144, Length=164, Percent_Identity=31.7073170731707, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571528, Length=318, Percent_Identity=35.5345911949685, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24585514, Length=328, Percent_Identity=28.9634146341463, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574282, Length=328, Percent_Identity=28.9634146341463, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574284, Length=328, Percent_Identity=28.9634146341463, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45551003, Length=328, Percent_Identity=28.9634146341463, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45552429, Length=328, Percent_Identity=28.9634146341463, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574286, Length=270, Percent_Identity=32.2222222222222, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24585516, Length=293, Percent_Identity=30.7167235494881, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19921140, Length=266, Percent_Identity=31.9548872180451, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24646446, Length=262, Percent_Identity=33.206106870229, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24646448, Length=262, Percent_Identity=33.206106870229, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24646452, Length=262, Percent_Identity=33.206106870229, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24646450, Length=262, Percent_Identity=33.206106870229, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI62472511, Length=262, Percent_Identity=33.206106870229, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 34193; Mature: 34061

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASERRRLLITRPMTAAVEARARAELDVEIRQETTPLSPEEMRQSLGAFDLVMPTLGDA
CCCCCCCEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHCCCCC
YSADVFAAVPQPRCRLLANFGVGFNHIDAEAARAAGVEVTNTPGAVTDATADIALTLMLM
CCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHH
TARRAGEGERLVRSGQWQGWHPTQMLGLHLSGKRLGVVGLGRIGDAIARRAHFGFGMEIS
HHHCCCCCHHHHHCCCCCCCCHHHEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEH
YLARSDKETGYPATRASNLIELAASVDMLVVAVPGGAETRHLINADVLAALPSHAHLVNI
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCHHHHHHCCCCCEEEEE
ARGEVVDEAALITALQAGQIAGAGLDVYEFEPKVPAELRAMEQVTLLPHLGTATEEVRSA
CCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
MGHMALDNCVAFLTGAPLPNPV
HHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AASERRRLLITRPMTAAVEARARAELDVEIRQETTPLSPEEMRQSLGAFDLVMPTLGDA
CCCCCCEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHCCCCC
YSADVFAAVPQPRCRLLANFGVGFNHIDAEAARAAGVEVTNTPGAVTDATADIALTLMLM
CCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHH
TARRAGEGERLVRSGQWQGWHPTQMLGLHLSGKRLGVVGLGRIGDAIARRAHFGFGMEIS
HHHCCCCCHHHHHCCCCCCCCHHHEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEH
YLARSDKETGYPATRASNLIELAASVDMLVVAVPGGAETRHLINADVLAALPSHAHLVNI
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCHHHHHHCCCCCEEEEE
ARGEVVDEAALITALQAGQIAGAGLDVYEFEPKVPAELRAMEQVTLLPHLGTATEEVRSA
CCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
MGHMALDNCVAFLTGAPLPNPV
HHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA