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The map label for this gene is proC [H]

Identifier: 99080464

GI number: 99080464

Start: 661804

End: 662619

Strand: Direct

Name: proC [H]

Synonym: TM1040_0623

Alternate gene names: 99080464

Gene position: 661804-662619 (Clockwise)

Preceding gene: 99080463

Following gene: 99080465

Centisome position: 20.68

GC content: 64.83

Gene sequence:

>816_bases
ATGGATATGAATGAGATCGCAGCGCGAGGGTTGGTGCTTTTGGGCTGCGGCAAAATGGGATCCGCGATGCTCAAGGGCTG
GCTCGAAGGGGGGCTGCCGCGTGAAGCTGTCTATGTAATGGACCCCAACCCCTCCGACTGGCTCAAGGGAACCGGCGTGC
ATATCAACGCCACGGTGCCTGAGCAGCCCGCAATCGTTCTGGTTGCGGTCAAGCCGCAGGTGATGGGCGCGGCGCTTCCT
TCTGCCAAATCCTATGGGGGCGGTGGGACCCTGTTTATCTCCGTTGCGGCAGGCACACCGATTTCGACGTTTGAGACGAT
GCTCGGTGAGAGCACCCCGATTGTACGCGCCATGCCCAACACACCCGCAGCCGTGGGGCGGGGCATTACGGCCCTCATCG
GGAACGCCCATGTGGATGAGGCCAAGATGGCGCAGGCCGACGCCCTGTTGCAGGCTGTGGGCCAGACGGTGCGGCTGGAC
GACGAAGGTCAGATGGATGCAGTCACCGGGGTGAGCGGATCCGGCCCTGCCTATGTCTTCCACCTCATTGAGACGCTGGC
CGCTGCGGGTGTGGCGCAGGGTCTGCCCGAGCCGCTGGCGATGCAACTGGCCAAGGCCACGGTTGCCGGAGCCGGGGCTC
TGGCTGAGGCGGCAGAAGAGGATCCCAGCGCGCTTCGGGTCAATGTGACCAGCCCGAATGGGACAACGCAGGCCGCGCTT
GAGGTCCTGATGGATGAGGAGACAGGCTTCCCGCCGCTCCTGCAGCGCGCGGTTGCGGCAGCGGCCAAGCGTTCGGAGGA
ACTGTCCCGTGGCTGA

Upstream 100 bases:

>100_bases
GATGCAGGTCGCCATTGCAGAGGCCTACGGCCGGGCGTAAGTCTTGTCCCCATCAGCCGCGCAAAAAGCGGCAATGTTCA
AACGATAGATGGGGGTAAGC

Downstream 100 bases:

>100_bases
CATTTCCTTTGATGACTTCCTGAAGGTCGACGTGCGCGTGGGGACTGTGGTGCGGGCCGAGCCCTATCCTGAAGCACGCC
GCCCTGCGATCAAGATGTGG

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MDMNEIAARGLVLLGCGKMGSAMLKGWLEGGLPREAVYVMDPNPSDWLKGTGVHINATVPEQPAIVLVAVKPQVMGAALP
SAKSYGGGGTLFISVAAGTPISTFETMLGESTPIVRAMPNTPAAVGRGITALIGNAHVDEAKMAQADALLQAVGQTVRLD
DEGQMDAVTGVSGSGPAYVFHLIETLAAAGVAQGLPEPLAMQLAKATVAGAGALAEAAEEDPSALRVNVTSPNGTTQAAL
EVLMDEETGFPPLLQRAVAAAAKRSEELSRG

Sequences:

>Translated_271_residues
MDMNEIAARGLVLLGCGKMGSAMLKGWLEGGLPREAVYVMDPNPSDWLKGTGVHINATVPEQPAIVLVAVKPQVMGAALP
SAKSYGGGGTLFISVAAGTPISTFETMLGESTPIVRAMPNTPAAVGRGITALIGNAHVDEAKMAQADALLQAVGQTVRLD
DEGQMDAVTGVSGSGPAYVFHLIETLAAAGVAQGLPEPLAMQLAKATVAGAGALAEAAEEDPSALRVNVTSPNGTTQAAL
EVLMDEETGFPPLLQRAVAAAAKRSEELSRG
>Mature_271_residues
MDMNEIAARGLVLLGCGKMGSAMLKGWLEGGLPREAVYVMDPNPSDWLKGTGVHINATVPEQPAIVLVAVKPQVMGAALP
SAKSYGGGGTLFISVAAGTPISTFETMLGESTPIVRAMPNTPAAVGRGITALIGNAHVDEAKMAQADALLQAVGQTVRLD
DEGQMDAVTGVSGSGPAYVFHLIETLAAAGVAQGLPEPLAMQLAKATVAGAGALAEAAEEDPSALRVNVTSPNGTTQAAL
EVLMDEETGFPPLLQRAVAAAAKRSEELSRG

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI24797097, Length=268, Percent_Identity=33.5820895522388, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI24797095, Length=268, Percent_Identity=33.5820895522388, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI198041662, Length=279, Percent_Identity=31.8996415770609, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI21361454, Length=270, Percent_Identity=32.5925925925926, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=36.0594795539033, Blast_Score=131, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17569021, Length=262, Percent_Identity=32.824427480916, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17540664, Length=283, Percent_Identity=27.9151943462898, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320861, Length=286, Percent_Identity=29.3706293706294, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21358587, Length=265, Percent_Identity=39.2452830188679, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24648116, Length=276, Percent_Identity=34.4202898550725, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24647700, Length=172, Percent_Identity=41.2790697674419, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 27563; Mature: 27563

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMNEIAARGLVLLGCGKMGSAMLKGWLEGGLPREAVYVMDPNPSDWLKGTGVHINATVP
CCHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCEEEEEECC
EQPAIVLVAVKPQVMGAALPSAKSYGGGGTLFISVAAGTPISTFETMLGESTPIVRAMPN
CCCCEEEEEECCHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCC
TPAAVGRGITALIGNAHVDEAKMAQADALLQAVGQTVRLDDEGQMDAVTGVSGSGPAYVF
CCHHHHCCHHEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCHHHHH
HLIETLAAAGVAQGLPEPLAMQLAKATVAGAGALAEAAEEDPSALRVNVTSPNGTTQAAL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCHHHHHH
EVLMDEETGFPPLLQRAVAAAAKRSEELSRG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDMNEIAARGLVLLGCGKMGSAMLKGWLEGGLPREAVYVMDPNPSDWLKGTGVHINATVP
CCHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCEEEEEECC
EQPAIVLVAVKPQVMGAALPSAKSYGGGGTLFISVAAGTPISTFETMLGESTPIVRAMPN
CCCCEEEEEECCHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCC
TPAAVGRGITALIGNAHVDEAKMAQADALLQAVGQTVRLDDEGQMDAVTGVSGSGPAYVF
CCHHHHCCHHEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCHHHHH
HLIETLAAAGVAQGLPEPLAMQLAKATVAGAGALAEAAEEDPSALRVNVTSPNGTTQAAL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCHHHHHH
EVLMDEETGFPPLLQRAVAAAAKRSEELSRG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2107123; 10984043; 1676385 [H]