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The map label for this gene is hipO [H]

Identifier: 99080353

GI number: 99080353

Start: 538014

End: 539153

Strand: Direct

Name: hipO [H]

Synonym: TM1040_0512

Alternate gene names: 99080353

Gene position: 538014-539153 (Clockwise)

Preceding gene: 99080352

Following gene: 99080354

Centisome position: 16.81

GC content: 63.86

Gene sequence:

>1140_bases
ATGTTCGACACGACGCATATCGAAGAGGCCATCGCCCTGCGCCATGACCTTCATCGCCACCCAGAGTTGGGGTTCGAAGA
GCATCGCACCTCCGAGATCATCGCGGGCCTTTTGCACGGCTGGGGCTGGCGTGTGCATCGTGGTCTCGCAGGGACAGGGG
TTGTTGCGCAGATGGGGCAGGGGGAACCGGTCATAGGTCTGCGCGCCGATATTGATGCATTGCCGATGGAGGAGGCTACA
GGGCTGGCCTATGCCTCGGGGACGCCCGGCAAGATGCATGCCTGCGGTCATGATGGTCACACCGCGATGTTGCTGCTGGC
TGCGCGCAAGATCGCCGAAGAGGGCGTGGCCACAGGCACGGTAACGCTGATCTTCCAACCCGCAGAGGAGAATGATGGCG
GCGCACGGGTCATGATTGAACAGGGGCTGTTTCGCGAGTTTCCGGTCGATCAGGTCTATGGCATTCACAACTGGCCGGGG
CTCGCGCCAGGGCGCATGGTGGCGCGTGACGACAAGATGATGGCGGCCTTTGCCGTCTTTGAGATCGAGGTTGCCGGGCA
GGGTGGGCATGGCGCAATGCCGGAGCAATCGGATGGGGTCATCGCGGCTGCAGCGGCGATGGCGTCGACGTTGCAGGAAA
TTCCTGCCCGCGCTTTGTCGCCGCTGGAGCCGGGCGTGGTCTCGGTGACGCAGATCCATTCCGGATCGGCGTGGAATGTC
TGCCCTGACCGTGCGGTCTTGCGCGGGACCGCGCGCTGGTTCGATCCCGCTGCTGGCGATACGATTGAGGCCCGTCTGAC
ACAGGTGGCGAACGCCTGCGCCGCCGCACAGGGGTGCAGCGCGCGTATTGATTACCAGCGTCGCTATCCCGCGACGATCA
ACTCTGCTGTCGAGGCCGCTGCGGCGCGCGCGGTGGCCGCCGAGATGGGGCTTGAGACAGCCAATGTGGCACCGAGCATG
GCATCAGAGGATTTTGCCTTTATGCTCAACGAGGTGTCCGGCGCCTATATCTGGCTTGGAGCGGCGCGCGACGGAGAAAA
TCCGGGTCTGCACTCAGCCAAATTTGATTTCAATGATGCCGTGCTCCCAGTCGGGGCCGAGTTCTGGGTGCGGCTGGCCC
GCCGCCAGCTGGCGACCTGA

Upstream 100 bases:

>100_bases
TCTGCCAGGACGTCAATGGGGCCTTGGTGCTGACAGAGGTTGCGCGTCTGGGTGGCATCCTTCTGAGTTTTCACGAAATA
CCAAAGGTTTGAGAGAGAGC

Downstream 100 bases:

>100_bases
TGGACGCGCTTGAGACAAAGTTCCTGCGCGAGTTTCTCGCGGTGGCGCATGAAGGTTCGATCCGGCGCGCGGCAGACCGG
CTCAACGTGGTGCCTTCTGC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MFDTTHIEEAIALRHDLHRHPELGFEEHRTSEIIAGLLHGWGWRVHRGLAGTGVVAQMGQGEPVIGLRADIDALPMEEAT
GLAYASGTPGKMHACGHDGHTAMLLLAARKIAEEGVATGTVTLIFQPAEENDGGARVMIEQGLFREFPVDQVYGIHNWPG
LAPGRMVARDDKMMAAFAVFEIEVAGQGGHGAMPEQSDGVIAAAAAMASTLQEIPARALSPLEPGVVSVTQIHSGSAWNV
CPDRAVLRGTARWFDPAAGDTIEARLTQVANACAAAQGCSARIDYQRRYPATINSAVEAAAARAVAAEMGLETANVAPSM
ASEDFAFMLNEVSGAYIWLGAARDGENPGLHSAKFDFNDAVLPVGAEFWVRLARRQLAT

Sequences:

>Translated_379_residues
MFDTTHIEEAIALRHDLHRHPELGFEEHRTSEIIAGLLHGWGWRVHRGLAGTGVVAQMGQGEPVIGLRADIDALPMEEAT
GLAYASGTPGKMHACGHDGHTAMLLLAARKIAEEGVATGTVTLIFQPAEENDGGARVMIEQGLFREFPVDQVYGIHNWPG
LAPGRMVARDDKMMAAFAVFEIEVAGQGGHGAMPEQSDGVIAAAAAMASTLQEIPARALSPLEPGVVSVTQIHSGSAWNV
CPDRAVLRGTARWFDPAAGDTIEARLTQVANACAAAQGCSARIDYQRRYPATINSAVEAAAARAVAAEMGLETANVAPSM
ASEDFAFMLNEVSGAYIWLGAARDGENPGLHSAKFDFNDAVLPVGAEFWVRLARRQLAT
>Mature_379_residues
MFDTTHIEEAIALRHDLHRHPELGFEEHRTSEIIAGLLHGWGWRVHRGLAGTGVVAQMGQGEPVIGLRADIDALPMEEAT
GLAYASGTPGKMHACGHDGHTAMLLLAARKIAEEGVATGTVTLIFQPAEENDGGARVMIEQGLFREFPVDQVYGIHNWPG
LAPGRMVARDDKMMAAFAVFEIEVAGQGGHGAMPEQSDGVIAAAAAMASTLQEIPARALSPLEPGVVSVTQIHSGSAWNV
CPDRAVLRGTARWFDPAAGDTIEARLTQVANACAAAQGCSARIDYQRRYPATINSAVEAAAARAVAAEMGLETANVAPSM
ASEDFAFMLNEVSGAYIWLGAARDGENPGLHSAKFDFNDAVLPVGAEFWVRLARRQLAT

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=431, Percent_Identity=28.538283062645, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 40234; Mature: 40234

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDTTHIEEAIALRHDLHRHPELGFEEHRTSEIIAGLLHGWGWRVHRGLAGTGVVAQMGQ
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCCC
GEPVIGLRADIDALPMEEATGLAYASGTPGKMHACGHDGHTAMLLLAARKIAEEGVATGT
CCCEEEEECCCCCCCCHHHCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEE
VTLIFQPAEENDGGARVMIEQGLFREFPVDQVYGIHNWPGLAPGRMVARDDKMMAAFAVF
EEEEEEECCCCCCCEEEEEECCCHHHCCHHHCCCCCCCCCCCCCCEEECCCHHEEEEEEE
EIEVAGQGGHGAMPEQSDGVIAAAAAMASTLQEIPARALSPLEPGVVSVTQIHSGSAWNV
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
CPDRAVLRGTARWFDPAAGDTIEARLTQVANACAAAQGCSARIDYQRRYPATINSAVEAA
CCCHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHH
AARAVAAEMGLETANVAPSMASEDFAFMLNEVSGAYIWLGAARDGENPGLHSAKFDFNDA
HHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCC
VLPVGAEFWVRLARRQLAT
EECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDTTHIEEAIALRHDLHRHPELGFEEHRTSEIIAGLLHGWGWRVHRGLAGTGVVAQMGQ
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCCC
GEPVIGLRADIDALPMEEATGLAYASGTPGKMHACGHDGHTAMLLLAARKIAEEGVATGT
CCCEEEEECCCCCCCCHHHCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEE
VTLIFQPAEENDGGARVMIEQGLFREFPVDQVYGIHNWPGLAPGRMVARDDKMMAAFAVF
EEEEEEECCCCCCCEEEEEECCCHHHCCHHHCCCCCCCCCCCCCCEEECCCHHEEEEEEE
EIEVAGQGGHGAMPEQSDGVIAAAAAMASTLQEIPARALSPLEPGVVSVTQIHSGSAWNV
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
CPDRAVLRGTARWFDPAAGDTIEARLTQVANACAAAQGCSARIDYQRRYPATINSAVEAA
CCCHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHH
AARAVAAEMGLETANVAPSMASEDFAFMLNEVSGAYIWLGAARDGENPGLHSAKFDFNDA
HHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCC
VLPVGAEFWVRLARRQLAT
EECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]