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The map label for this gene is ampR [H]

Identifier: 99080163

GI number: 99080163

Start: 326868

End: 327740

Strand: Reverse

Name: ampR [H]

Synonym: TM1040_0322

Alternate gene names: 99080163

Gene position: 327740-326868 (Counterclockwise)

Preceding gene: 99080171

Following gene: 99080154

Centisome position: 10.24

GC content: 63.0

Gene sequence:

>873_bases
ATGCGTAAACTCCCGCCGCTCAACGCAATTCGTGCCTTCGAGGCTGTCGCCAGACGGCTCAGTTTTGCCGATGCGGGCGA
TGAACTCGGGGTGACGGCCACCGCGATCAGCCACCAGATCAGGCATCTTGAGGCCTATCTCGGGTTTCAACTGATTGAGC
GCCGCCCCCGCCAGATCGCCCTCACCCCCGCAGGCCAGGAATTGTTTCCCAAGCTGCAGATGGCGTTCGACACACTCGGA
GAAGCCTTTGCGATGCTCGACAAGGATGTGGAGCAATCCCTGGTGCGGGTCACGGCCACCTATGCCTTTGCCGAGCGTTG
GCTGGTGCCGCGCCTGACGGGGTTTTTCGAGCAGCACCCCGACATCCGCGTCGAGGTGGAGACGGATGACGCCATCCTTG
ATTTACGCGCAGATCGGCTGGATTTGGCGATCCGCTACGGGCCCCAGACCGCGGCAACGGAACACGCCGAAGTCCTGTTC
TCGGACCGTTTCATCCCCCTGCGGCTGGCCAGCCAGCCCGAGGCGGACCTGCAAGAAAGCCGCCTGCTGGGATTTCGCTG
GCGCAATGCGGATATGGGCGGGCCAGTCTGGGAAGATTGGTTCAAGGCAGCCGGAGAGCCCTTCCCCGCCGAACGCATGA
CCCGCTTCAGCGATGAAAGCTCGGCCTTCAATGCGCTGGACCGAGGGTTGGGGCTGCTTTTGTCCAGCAGCGCGCTCGCC
GACGAAGGCCTGCAGTCGGGTCGCTACCAACAGGTCCCCGGCCCCGAGCTTCACGGGTTTGCCTTTTACCTCGTGCGCAA
CCCGCTGTCGCGCAACAAACAGGGTGTAAGGGTCTTCACCGATTGGATCAGAGAGGCCGCCAAGGAGAGCTGA

Upstream 100 bases:

>100_bases
CCTCCCTTTTCATTCCACAAGCTTCAGAAAGAGGAGGCCGAAAAACAAATGACCATTCGTCAGCCCATGCTGAGATTTCT
TTTCTCACCCGAGGCGTGCA

Downstream 100 bases:

>100_bases
GCGACGCGAGGCCCCCACAGAGTTCTGCAACAGCTTCCCGAATGGATCAGATCACAGCGACGGTCCGGGCGTCGCGGGAT
CGGGCCTCAACCTCTGGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRKLPPLNAIRAFEAVARRLSFADAGDELGVTATAISHQIRHLEAYLGFQLIERRPRQIALTPAGQELFPKLQMAFDTLG
EAFAMLDKDVEQSLVRVTATYAFAERWLVPRLTGFFEQHPDIRVEVETDDAILDLRADRLDLAIRYGPQTAATEHAEVLF
SDRFIPLRLASQPEADLQESRLLGFRWRNADMGGPVWEDWFKAAGEPFPAERMTRFSDESSAFNALDRGLGLLLSSSALA
DEGLQSGRYQQVPGPELHGFAFYLVRNPLSRNKQGVRVFTDWIREAAKES

Sequences:

>Translated_290_residues
MRKLPPLNAIRAFEAVARRLSFADAGDELGVTATAISHQIRHLEAYLGFQLIERRPRQIALTPAGQELFPKLQMAFDTLG
EAFAMLDKDVEQSLVRVTATYAFAERWLVPRLTGFFEQHPDIRVEVETDDAILDLRADRLDLAIRYGPQTAATEHAEVLF
SDRFIPLRLASQPEADLQESRLLGFRWRNADMGGPVWEDWFKAAGEPFPAERMTRFSDESSAFNALDRGLGLLLSSSALA
DEGLQSGRYQQVPGPELHGFAFYLVRNPLSRNKQGVRVFTDWIREAAKES
>Mature_290_residues
MRKLPPLNAIRAFEAVARRLSFADAGDELGVTATAISHQIRHLEAYLGFQLIERRPRQIALTPAGQELFPKLQMAFDTLG
EAFAMLDKDVEQSLVRVTATYAFAERWLVPRLTGFFEQHPDIRVEVETDDAILDLRADRLDLAIRYGPQTAATEHAEVLF
SDRFIPLRLASQPEADLQESRLLGFRWRNADMGGPVWEDWFKAAGEPFPAERMTRFSDESSAFNALDRGLGLLLSSSALA
DEGLQSGRYQQVPGPELHGFAFYLVRNPLSRNKQGVRVFTDWIREAAKES

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=31.9727891156463, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=30.8510638297872, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=26.6666666666667, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787128, Length=139, Percent_Identity=39.568345323741, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI145693193, Length=305, Percent_Identity=26.5573770491803, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081978, Length=208, Percent_Identity=28.3653846153846, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786401, Length=183, Percent_Identity=27.8688524590164, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787589, Length=165, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789440, Length=279, Percent_Identity=25.089605734767, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32705; Mature: 32705

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPPLNAIRAFEAVARRLSFADAGDELGVTATAISHQIRHLEAYLGFQLIERRPRQIA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTPAGQELFPKLQMAFDTLGEAFAMLDKDVEQSLVRVTATYAFAERWLVPRLTGFFEQHP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIRVEVETDDAILDLRADRLDLAIRYGPQTAATEHAEVLFSDRFIPLRLASQPEADLQES
CEEEEEECCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
RLLGFRWRNADMGGPVWEDWFKAAGEPFPAERMTRFSDESSAFNALDRGLGLLLSSSALA
HHHEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DEGLQSGRYQQVPGPELHGFAFYLVRNPLSRNKQGVRVFTDWIREAAKES
HHHHHCCCCCCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLPPLNAIRAFEAVARRLSFADAGDELGVTATAISHQIRHLEAYLGFQLIERRPRQIA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTPAGQELFPKLQMAFDTLGEAFAMLDKDVEQSLVRVTATYAFAERWLVPRLTGFFEQHP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIRVEVETDDAILDLRADRLDLAIRYGPQTAATEHAEVLFSDRFIPLRLASQPEADLQES
CEEEEEECCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
RLLGFRWRNADMGGPVWEDWFKAAGEPFPAERMTRFSDESSAFNALDRGLGLLLSSSALA
HHHEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DEGLQSGRYQQVPGPELHGFAFYLVRNPLSRNKQGVRVFTDWIREAAKES
HHHHHCCCCCCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8405939; 10984043; 2125210 [H]