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The map label for this gene is ttcA

Identifier: 99080150

GI number: 99080150

Start: 315963

End: 316850

Strand: Reverse

Name: ttcA

Synonym: TM1040_0309

Alternate gene names: 99080150

Gene position: 316850-315963 (Counterclockwise)

Preceding gene: 99080152

Following gene: 99080149

Centisome position: 9.9

GC content: 59.12

Gene sequence:

>888_bases
ATGTTGGACCAGGATCTCGACGATATTCACCCGCTCTTTCAGGGCGCGCCACAAACCACCGAGTTCAAGAAACTCCGCAA
ACGCATCGTGCGCTACACCCGCGAGGCGGTGGAGCAATATGGCATGGTTGAACGGCGCGAGGATGGCTCCCTGCCAAAAT
GGCTGGTGTGCCTTTCGGGCGGCAAGGACAGCTACACGCTCTTGGCCGTACTCTACGAGCTCAAGTGGCGCGGATTGCTG
CCGGTGGATCTCTTGGCCTGCAATCTCGACCAGGGCCAGCCGGGGTTTCCCGCGACGGTGCTGCCGGAGTTTCTGGAAAA
GATGCAGGTGCCACACCGGATCGAATACCAGGACACCTACTCCATCGTGATGGATAAAGTCCCGCAGGGGCGCACATATT
GCGCGCTCTGCTCGCGTCTGCGTCGCGGCAATCTCTACCGCATCGCCCGCGAGGAGGGCTGCTCTGCAGTGGTGCTTGGT
CATCATCGCGACGACATTCTCGAGACGTTCTTCATGAACCTGTTTCACGGCGGGCGCCTCGCCACGATGCCGCCAAAGCT
GGTGAACGAAGAGGGGGACCTCTTTGTGTTTCGCCCGCTCGCACATGTGGCCGAGGCCGATTGCGAGAAATTTGCCCGCG
CGATGAACTATCCGATCATTCCCTGCGATCTCTGCGGCAGCCAGGATGGGCTGCAGCGCCAGCAGGTGAAACAGATCCTT
GATACCTGGGAATCAAACAGCCCCGGACGGCGGCAGGTGATGTTCCGGGCGCTCATGAATGCGCGTCCGTCGCATCTTCT
GGACCCGAAGCTCTTTGATTTCACGGGGCTTGCGCTGAAAAATATCGATAATTCGACGGAAACTGAAGAAATCCCCGAGC
TGCGTTAA

Upstream 100 bases:

>100_bases
GCCCCAGCGGTGGCTACGGATCCAAAATCATCGCTGACTTTGCGCAGCCGAGATGCAGGTTTTACTTGGCAAGCCTCCTG
ACAGGCGCTACGGGGCGCAC

Downstream 100 bases:

>100_bases
CTCCTCAGTCAGACCCGCGACCTAGTCTATGCCTGCACCACCGGCGAAGTCGAAAGGTCTCGAACTCATGAATTTTCCGT
ATTCACGCCTTCTACTGCGG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLDQDLDDIHPLFQGAPQTTEFKKLRKRIVRYTREAVEQYGMVERREDGSLPKWLVCLSGGKDSYTLLAVLYELKWRGLL
PVDLLACNLDQGQPGFPATVLPEFLEKMQVPHRIEYQDTYSIVMDKVPQGRTYCALCSRLRRGNLYRIAREEGCSAVVLG
HHRDDILETFFMNLFHGGRLATMPPKLVNEEGDLFVFRPLAHVAEADCEKFARAMNYPIIPCDLCGSQDGLQRQQVKQIL
DTWESNSPGRRQVMFRALMNARPSHLLDPKLFDFTGLALKNIDNSTETEEIPELR

Sequences:

>Translated_295_residues
MLDQDLDDIHPLFQGAPQTTEFKKLRKRIVRYTREAVEQYGMVERREDGSLPKWLVCLSGGKDSYTLLAVLYELKWRGLL
PVDLLACNLDQGQPGFPATVLPEFLEKMQVPHRIEYQDTYSIVMDKVPQGRTYCALCSRLRRGNLYRIAREEGCSAVVLG
HHRDDILETFFMNLFHGGRLATMPPKLVNEEGDLFVFRPLAHVAEADCEKFARAMNYPIIPCDLCGSQDGLQRQQVKQIL
DTWESNSPGRRQVMFRALMNARPSHLLDPKLFDFTGLALKNIDNSTETEEIPELR
>Mature_295_residues
MLDQDLDDIHPLFQGAPQTTEFKKLRKRIVRYTREAVEQYGMVERREDGSLPKWLVCLSGGKDSYTLLAVLYELKWRGLL
PVDLLACNLDQGQPGFPATVLPEFLEKMQVPHRIEYQDTYSIVMDKVPQGRTYCALCSRLRRGNLYRIAREEGCSAVVLG
HHRDDILETFFMNLFHGGRLATMPPKLVNEEGDLFVFRPLAHVAEADCEKFARAMNYPIIPCDLCGSQDGLQRQQVKQIL
DTWESNSPGRRQVMFRALMNARPSHLLDPKLFDFTGLALKNIDNSTETEEIPELR

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=276, Percent_Identity=48.9130434782609, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI193211090, Length=223, Percent_Identity=34.0807174887892, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17540090, Length=166, Percent_Identity=33.1325301204819, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_SILST (Q1GJX4)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612304.1
- ProteinModelPortal:   Q1GJX4
- STRING:   Q1GJX4
- GeneID:   4077132
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0309
- NMPDR:   fig|292414.1.peg.2508
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   FPHAREE
- PhylomeDB:   Q1GJX4
- ProtClustDB:   PRK10696
- BioCyc:   SSP292414:TM1040_0309-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 33844; Mature: 33844

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQDLDDIHPLFQGAPQTTEFKKLRKRIVRYTREAVEQYGMVERREDGSLPKWLVCLSG
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
GKDSYTLLAVLYELKWRGLLPVDLLACNLDQGQPGFPATVLPEFLEKMQVPHRIEYQDTY
CCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SIVMDKVPQGRTYCALCSRLRRGNLYRIAREEGCSAVVLGHHRDDILETFFMNLFHGGRL
HHHHHCCCCCCHHHHHHHHHHCCCEEEEHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEE
ATMPPKLVNEEGDLFVFRPLAHVAEADCEKFARAMNYPIIPCDLCGSQDGLQRQQVKQIL
CCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCHHHHHHHHHH
DTWESNSPGRRQVMFRALMNARPSHLLDPKLFDFTGLALKNIDNSTETEEIPELR
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCC
>Mature Secondary Structure
MLDQDLDDIHPLFQGAPQTTEFKKLRKRIVRYTREAVEQYGMVERREDGSLPKWLVCLSG
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
GKDSYTLLAVLYELKWRGLLPVDLLACNLDQGQPGFPATVLPEFLEKMQVPHRIEYQDTY
CCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SIVMDKVPQGRTYCALCSRLRRGNLYRIAREEGCSAVVLGHHRDDILETFFMNLFHGGRL
HHHHHCCCCCCHHHHHHHHHHCCCEEEEHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEE
ATMPPKLVNEEGDLFVFRPLAHVAEADCEKFARAMNYPIIPCDLCGSQDGLQRQQVKQIL
CCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCHHHHHHHHHH
DTWESNSPGRRQVMFRALMNARPSHLLDPKLFDFTGLALKNIDNSTETEEIPELR
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA