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The map label for this gene is nfnB [C]

Identifier: 99080037

GI number: 99080037

Start: 214406

End: 215035

Strand: Reverse

Name: nfnB [C]

Synonym: TM1040_0196

Alternate gene names: 99080037

Gene position: 215035-214406 (Counterclockwise)

Preceding gene: 99080038

Following gene: 99080030

Centisome position: 6.72

GC content: 59.52

Gene sequence:

>630_bases
ATGTTTTTGGACAAGCTGAACTGGCGCTATGCCGCCAAGAAAATGGACCCCTCAAAGCCCGTATCCGAGGATAAGGTCGC
TCAGATCGTTGAGGCCATTCGCATGGCGCCGACCTCCAGCGGCGTGCAGCCGTTTGAACTCTTGGTCATTCGCAACAAGG
ACCTGCGCGAGAAAATCCGCCCGGTGTCCTGGGATCAGGCCCAGATCACAGATGGCTCCCATCTTCTGGTCTTTGCTGCC
TGGGACAATTACACCGAAGAGCGCATTGACGCGGTCGTGGCGCAGATGGTCGAAGAGCGCGGCGATTCCGAGTTGCTTCA
GAACTACTTTGCCAATCTCAAGGAGATGCTCCTGCCGCGCCCCGCAGAGGTGAACCACGCCCATGCAGCCCATCAGGCCT
ATATCGCGCTTGGCTTTGCACTGGCCGCCGCCGCAGAACTCGGCGTCGACAGCACCCCGATGGAAGGGTTTGACCCCGAT
CAGGTGGATGAGATCCTCGACCTGCGCGCAAAGGGCCTGCGCGCCAAGGTTCTGTTGCCGCTCGGCACCCGGGCAGAAGA
GGGCGACTGGTTGTTGCCAATGCCGAAAGTGCGCAAATCCAAAGCCGCACTGGTCACTGAGATCGACTAA

Upstream 100 bases:

>100_bases
CCCTTGGCGTCTAAATAAATCTCGACGTCGAGATAAATCAACTTGAACTGTTCAGTTTAGTTGTTATGTCTGCGGCAACA
TGAAGAAAAGGATGTCTCCC

Downstream 100 bases:

>100_bases
TCAATGCGAAAAGCCCCCTGCCCTCTGTGGCGGGGGGATGCTCTATCGGACAAGCTGCCTCTTGCAGATCAGTCCTGACG
CTGGAACGTCAGGTAATGCG

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MFLDKLNWRYAAKKMDPSKPVSEDKVAQIVEAIRMAPTSSGVQPFELLVIRNKDLREKIRPVSWDQAQITDGSHLLVFAA
WDNYTEERIDAVVAQMVEERGDSELLQNYFANLKEMLLPRPAEVNHAHAAHQAYIALGFALAAAAELGVDSTPMEGFDPD
QVDEILDLRAKGLRAKVLLPLGTRAEEGDWLLPMPKVRKSKAALVTEID

Sequences:

>Translated_209_residues
MFLDKLNWRYAAKKMDPSKPVSEDKVAQIVEAIRMAPTSSGVQPFELLVIRNKDLREKIRPVSWDQAQITDGSHLLVFAA
WDNYTEERIDAVVAQMVEERGDSELLQNYFANLKEMLLPRPAEVNHAHAAHQAYIALGFALAAAAELGVDSTPMEGFDPD
QVDEILDLRAKGLRAKVLLPLGTRAEEGDWLLPMPKVRKSKAALVTEID
>Mature_209_residues
MFLDKLNWRYAAKKMDPSKPVSEDKVAQIVEAIRMAPTSSGVQPFELLVIRNKDLREKIRPVSWDQAQITDGSHLLVFAA
WDNYTEERIDAVVAQMVEERGDSELLQNYFANLKEMLLPRPAEVNHAHAAHQAYIALGFALAAAAELGVDSTPMEGFDPD
QVDEILDLRAKGLRAKVLLPLGTRAEEGDWLLPMPKVRKSKAALVTEID

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=213, Percent_Identity=25.8215962441315, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23361; Mature: 23361

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLDKLNWRYAAKKMDPSKPVSEDKVAQIVEAIRMAPTSSGVQPFELLVIRNKDLREKIR
CCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCC
PVSWDQAQITDGSHLLVFAAWDNYTEERIDAVVAQMVEERGDSELLQNYFANLKEMLLPR
CCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
PAEVNHAHAAHQAYIALGFALAAAAELGVDSTPMEGFDPDQVDEILDLRAKGLRAKVLLP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEE
LGTRAEEGDWLLPMPKVRKSKAALVTEID
CCCCCCCCCEECCCCHHHHCHHHEEEECC
>Mature Secondary Structure
MFLDKLNWRYAAKKMDPSKPVSEDKVAQIVEAIRMAPTSSGVQPFELLVIRNKDLREKIR
CCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCC
PVSWDQAQITDGSHLLVFAAWDNYTEERIDAVVAQMVEERGDSELLQNYFANLKEMLLPR
CCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
PAEVNHAHAAHQAYIALGFALAAAAELGVDSTPMEGFDPDQVDEILDLRAKGLRAKVLLP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEE
LGTRAEEGDWLLPMPKVRKSKAALVTEID
CCCCCCCCCEECCCCHHHHCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]