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The map label for this gene is yeiR [C]

Identifier: 99079964

GI number: 99079964

Start: 130689

End: 131552

Strand: Reverse

Name: yeiR [C]

Synonym: TM1040_0123

Alternate gene names: 99079964

Gene position: 131552-130689 (Counterclockwise)

Preceding gene: 99079965

Following gene: 99079957

Centisome position: 4.11

GC content: 59.84

Gene sequence:

>864_bases
ATGCGTATTCCTGTCACAATCATCAGCGGTTATCTCGGAGCAGGAAAGACCACATTGATCAACTCGCTGCTCGGCGAGGA
TCACGGGCTGCGCCTCTCTGTCTTGATCAATGACTTTGGCAGCATCAACATCGACGAGGCCCTGATCTCGAACAAGACTG
TCGACAGCGTCGCGCTCACCAATGGCTGCGTGTGCTGCTCGCTGGGAGATGATCTTGCACTGAGTGTGAATGACCTTGTG
TCTCGTGCTGAGCCACCGGATCACATCCTGATCGAAACCAGCGGTGTGGCCGAACCCGTGGCCATCGCCAATGCGCTGCT
AAATGACAGCCGCCTGAGCTATGCGGGCATTGTTACGGTTGTGGATGGTTCGAATTTCTCCACACTCATGGACGACCCCT
TGTTGAAGCCACAGGTCACGCAGCAGATCTGTGCGGCGGACCTGGTACTGGTGAGCAAGCTAGAACACGAGAGTGATGAC
GGCGTCATCGCGGCACTCGGCGCACTCGGCGCTCGCACGCCCACGCTGACATCTGACATCGAACTCTCTCGTCTGCTATT
CGACATCACGCCGCTTCCACGCAGCAAAAGCACCGTTTCCCATCCCGGCTACGTCAGCTGGCATCACCGCAGCACCGAAG
TCATCGACCGCCGCGCCCTCGGCGACAAGCTCGCGGAGCGCCCGGAGGGGCTCTATCGCATGAAAGGTTTTGTGCTGACT
TCCGGCGGGGCTTACCAGCTCCATATCGTTGGCCGACATGTCGAAGCCCGCCGCTGCGACGCCAATGAAACCCTCCTGGT
GGCGCTGGGGCCCGAGCAGCGGATCAGCCACGACCAGATCGAGGCCTGGTGGACCGGCAGCTAG

Upstream 100 bases:

>100_bases
GAATCGCTCCCAGCGAGCCACAGAGTTTGCAGTTGCACACAATAGGGAGCTCGGCTGAGATGCTGAATACGTTGCATTCA
ATGCGCGGGGATAGGGTTTG

Downstream 100 bases:

>100_bases
GGCCTAACTGCGCCGCCGGACCTGACGACACACCAGGATCACGGGCAACAGTCCAACGGCCATGATCACAAGAGAGGGCA
CCGCCGCGCCCTCGAGCCGC

Product: cobalamin synthesis protein, P47K

Products: NA

Alternate protein names: P47K [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRIPVTIISGYLGAGKTTLINSLLGEDHGLRLSVLINDFGSINIDEALISNKTVDSVALTNGCVCCSLGDDLALSVNDLV
SRAEPPDHILIETSGVAEPVAIANALLNDSRLSYAGIVTVVDGSNFSTLMDDPLLKPQVTQQICAADLVLVSKLEHESDD
GVIAALGALGARTPTLTSDIELSRLLFDITPLPRSKSTVSHPGYVSWHHRSTEVIDRRALGDKLAERPEGLYRMKGFVLT
SGGAYQLHIVGRHVEARRCDANETLLVALGPEQRISHDQIEAWWTGS

Sequences:

>Translated_287_residues
MRIPVTIISGYLGAGKTTLINSLLGEDHGLRLSVLINDFGSINIDEALISNKTVDSVALTNGCVCCSLGDDLALSVNDLV
SRAEPPDHILIETSGVAEPVAIANALLNDSRLSYAGIVTVVDGSNFSTLMDDPLLKPQVTQQICAADLVLVSKLEHESDD
GVIAALGALGARTPTLTSDIELSRLLFDITPLPRSKSTVSHPGYVSWHHRSTEVIDRRALGDKLAERPEGLYRMKGFVLT
SGGAYQLHIVGRHVEARRCDANETLLVALGPEQRISHDQIEAWWTGS
>Mature_287_residues
MRIPVTIISGYLGAGKTTLINSLLGEDHGLRLSVLINDFGSINIDEALISNKTVDSVALTNGCVCCSLGDDLALSVNDLV
SRAEPPDHILIETSGVAEPVAIANALLNDSRLSYAGIVTVVDGSNFSTLMDDPLLKPQVTQQICAADLVLVSKLEHESDD
GVIAALGALGARTPTLTSDIELSRLLFDITPLPRSKSTVSHPGYVSWHHRSTEVIDRRALGDKLAERPEGLYRMKGFVLT
SGGAYQLHIVGRHVEARRCDANETLLVALGPEQRISHDQIEAWWTGS

Specific function: The presence of P47K is critical for the expression of the nitrile hydratase genes. May stabilize or activate the nitrile hydratase proteins [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI33469141, Length=204, Percent_Identity=33.8235294117647, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI126722884, Length=204, Percent_Identity=33.8235294117647, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI223941779, Length=165, Percent_Identity=36.3636363636364, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI148727351, Length=204, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI146231952, Length=204, Percent_Identity=32.843137254902, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI119120938, Length=130, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI223941776, Length=193, Percent_Identity=31.0880829015544, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI87082430, Length=211, Percent_Identity=30.8056872037915, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788499, Length=103, Percent_Identity=35.9223300970874, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324356, Length=141, Percent_Identity=38.2978723404255, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 30824; Mature: 30824

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPVTIISGYLGAGKTTLINSLLGEDHGLRLSVLINDFGSINIDEALISNKTVDSVALT
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCEECC
NGCVCCSLGDDLALSVNDLVSRAEPPDHILIETSGVAEPVAIANALLNDSRLSYAGIVTV
CCEEEEECCCCEEEEHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEE
VDGSNFSTLMDDPLLKPQVTQQICAADLVLVSKLEHESDDGVIAALGALGARTPTLTSDI
EECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCCCCCH
ELSRLLFDITPLPRSKSTVSHPGYVSWHHRSTEVIDRRALGDKLAERPEGLYRMKGFVLT
HHHHHHEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
SGGAYQLHIVGRHVEARRCDANETLLVALGPEQRISHDQIEAWWTGS
CCCEEEEEEEECCCCHHCCCCCCEEEEEECCHHHCCCCCEEEEECCC
>Mature Secondary Structure
MRIPVTIISGYLGAGKTTLINSLLGEDHGLRLSVLINDFGSINIDEALISNKTVDSVALT
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCEECC
NGCVCCSLGDDLALSVNDLVSRAEPPDHILIETSGVAEPVAIANALLNDSRLSYAGIVTV
CCEEEEECCCCEEEEHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEE
VDGSNFSTLMDDPLLKPQVTQQICAADLVLVSKLEHESDDGVIAALGALGARTPTLTSDI
EECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCCCCCH
ELSRLLFDITPLPRSKSTVSHPGYVSWHHRSTEVIDRRALGDKLAERPEGLYRMKGFVLT
HHHHHHEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
SGGAYQLHIVGRHVEARRCDANETLLVALGPEQRISHDQIEAWWTGS
CCCEEEEEEEECCCCHHCCCCCCEEEEEECCHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]