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The map label for this gene is truB

Identifier: 99079925

GI number: 99079925

Start: 87460

End: 88368

Strand: Reverse

Name: truB

Synonym: TM1040_0084

Alternate gene names: 99079925

Gene position: 88368-87460 (Counterclockwise)

Preceding gene: 99079926

Following gene: 99079924

Centisome position: 2.76

GC content: 60.84

Gene sequence:

>909_bases
ATGGGACGCAAACGCAAGGGACGCGACATTTCCGGCTGGTTGGTGGTGGACAAACCCGCAGGCCTCACCTCGACGGCGGT
GGTCAACAAGGTCCGTTGGGCGTTCGACGCCAAAAAAGCAGGCCATGCCGGTACGCTCGACCCCGAGGCAACCGGTGTGC
TTGCGGTGGCACTTGGCGAGGCGACAAAGACCGTGCCGTTTATCACCGACGCCATGAAAGCCTATACGTTTACCGTGACC
CTTGGGGCGGCGACCAATACGGATGACGCCGAAGGCGAAGTGATTGCGACCTCCGCAGAGCGCCCCACGGATGATGACAT
CAAAGAAGCACTGCTTGGCTTTATTGGCGAAATCCAGCAGGTGCCGCCGAAGTTCTCCGCTGTAAAAATCGACGGTCAGC
GCGCCTACAAGCTGGCGCGGGACGGCGAAGAAGTGGAGCTTTCCGCCCGTCCGCTCTGGGTCGAAGAGCTGGTGATGCTG
GATCGTCCCGACGCCGATCATGTTGTTCTGGAAATGACGTGCGGCAAGGGCGGCTACGTACGTTCCATCGCGCGCGATTT
GGGCGAAAAACTCGGCTGCCTTGGCCATGTTCGAGAGCTGCGCCGGATCTGGTCCGGCCCGTTTGATGCAGAAGATGGCG
TGACCCTTGAACAGATTGACGCGCTTGCAAAATCACCCGAGATCGACCAGTTCCTGCGGCCGCTTGAGGAGGGGCTTGCG
GATCTGCCCGAGCTGAAATGCACACCGCAAGGCGCGACCCGACTGCGCAACGGCAACCCGGGCATGGTGATCGCCTCTGA
CGTGGAATATGGCGAGGAAGCCTGGGCTTCTCTTGAAGGCAAAGCCGTGGCCGTGGGCACCTTCAAGGCCGGAGAGCTGC
ACCCTTCGCGCGTTTTCAATCAGGACTGA

Upstream 100 bases:

>100_bases
CGAAGATCGCGACACGAACGAAGGCGACACGCTACAGAATTGACAGCCCGTCAGTCCTGCGCTACGCCGCGCGCCTTATC
CGACATTTCTGAGGGCATTC

Downstream 100 bases:

>100_bases
GGCGCTTTACCCGCGTTGCGCGCACGCGCCATTCACGCTCGCACAACCAAGATTAAGACACTGTAAAGTATAGGGAAAAT
CCGCTGACCCATTGTTTCGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MGRKRKGRDISGWLVVDKPAGLTSTAVVNKVRWAFDAKKAGHAGTLDPEATGVLAVALGEATKTVPFITDAMKAYTFTVT
LGAATNTDDAEGEVIATSAERPTDDDIKEALLGFIGEIQQVPPKFSAVKIDGQRAYKLARDGEEVELSARPLWVEELVML
DRPDADHVVLEMTCGKGGYVRSIARDLGEKLGCLGHVRELRRIWSGPFDAEDGVTLEQIDALAKSPEIDQFLRPLEEGLA
DLPELKCTPQGATRLRNGNPGMVIASDVEYGEEAWASLEGKAVAVGTFKAGELHPSRVFNQD

Sequences:

>Translated_302_residues
MGRKRKGRDISGWLVVDKPAGLTSTAVVNKVRWAFDAKKAGHAGTLDPEATGVLAVALGEATKTVPFITDAMKAYTFTVT
LGAATNTDDAEGEVIATSAERPTDDDIKEALLGFIGEIQQVPPKFSAVKIDGQRAYKLARDGEEVELSARPLWVEELVML
DRPDADHVVLEMTCGKGGYVRSIARDLGEKLGCLGHVRELRRIWSGPFDAEDGVTLEQIDALAKSPEIDQFLRPLEEGLA
DLPELKCTPQGATRLRNGNPGMVIASDVEYGEEAWASLEGKAVAVGTFKAGELHPSRVFNQD
>Mature_301_residues
GRKRKGRDISGWLVVDKPAGLTSTAVVNKVRWAFDAKKAGHAGTLDPEATGVLAVALGEATKTVPFITDAMKAYTFTVTL
GAATNTDDAEGEVIATSAERPTDDDIKEALLGFIGEIQQVPPKFSAVKIDGQRAYKLARDGEEVELSARPLWVEELVMLD
RPDADHVVLEMTCGKGGYVRSIARDLGEKLGCLGHVRELRRIWSGPFDAEDGVTLEQIDALAKSPEIDQFLRPLEEGLAD
LPELKCTPQGATRLRNGNPGMVIASDVEYGEEAWASLEGKAVAVGTFKAGELHPSRVFNQD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=255, Percent_Identity=37.2549019607843, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4503337, Length=243, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI215599015, Length=243, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI2367200, Length=308, Percent_Identity=36.6883116883117, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17553978, Length=290, Percent_Identity=27.2413793103448, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=220, Percent_Identity=30, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=118, Percent_Identity=33.8983050847458, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364189, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364187, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364185, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364183, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI62471759, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17975520, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_SILST (Q1GKJ9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612079.1
- ProteinModelPortal:   Q1GKJ9
- SMR:   Q1GKJ9
- STRING:   Q1GKJ9
- GeneID:   4078750
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0084
- NMPDR:   fig|292414.1.peg.1136
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q1GKJ9
- ProtClustDB:   PRK05389
- BioCyc:   SSP292414:TM1040_0084-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32570; Mature: 32439

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKRKGRDISGWLVVDKPAGLTSTAVVNKVRWAFDAKKAGHAGTLDPEATGVLAVALGE
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
ATKTVPFITDAMKAYTFTVTLGAATNTDDAEGEVIATSAERPTDDDIKEALLGFIGEIQQ
CCCCCHHHHHHHEEEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
VPPKFSAVKIDGQRAYKLARDGEEVELSARPLWVEELVMLDRPDADHVVLEMTCGKGGYV
CCCCCEEEEECCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCHHH
RSIARDLGEKLGCLGHVRELRRIWSGPFDAEDGVTLEQIDALAKSPEIDQFLRPLEEGLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
DLPELKCTPQGATRLRNGNPGMVIASDVEYGEEAWASLEGKAVAVGTFKAGELHPSRVFN
HCCCCCCCCCHHHHCCCCCCCEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCHHHCCC
QD
CC
>Mature Secondary Structure 
GRKRKGRDISGWLVVDKPAGLTSTAVVNKVRWAFDAKKAGHAGTLDPEATGVLAVALGE
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
ATKTVPFITDAMKAYTFTVTLGAATNTDDAEGEVIATSAERPTDDDIKEALLGFIGEIQQ
CCCCCHHHHHHHEEEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
VPPKFSAVKIDGQRAYKLARDGEEVELSARPLWVEELVMLDRPDADHVVLEMTCGKGGYV
CCCCCEEEEECCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCHHH
RSIARDLGEKLGCLGHVRELRRIWSGPFDAEDGVTLEQIDALAKSPEIDQFLRPLEEGLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
DLPELKCTPQGATRLRNGNPGMVIASDVEYGEEAWASLEGKAVAVGTFKAGELHPSRVFN
HCCCCCCCCCHHHHCCCCCCCEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCHHHCCC
QD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA