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The map label for this gene is birA [H]

Identifier: 94994605

GI number: 94994605

Start: 1157968

End: 1158909

Strand: Direct

Name: birA [H]

Synonym: MGAS10750_Spy1209

Alternate gene names: 94994605

Gene position: 1157968-1158909 (Clockwise)

Preceding gene: 94994590

Following gene: 94994610

Centisome position: 59.78

GC content: 40.66

Gene sequence:

>942_bases
ATGAAAACATCAGAAAAAATTTATCAATTACTGTCACAAACCGATGACTTCGTCAGTGGGGAATATCTAGCAGATCAACT
CAGTATCTCTCGTACATCCGTTTGGAAATCCATCAAAAGTCTCGAAAATCAGGGGATTCAAATTGATTCTTTAAAACACA
AGGGCTACCGCATGGTACAAGGTGATATTTTATTACCCAAAACCATTAGCCAAGGGCTTGGGATGCCAGTCACCTATACC
CCTCATAGCCAATCTACCCAATTAGATGCCAAACAAGGAATCGAAGCTCATAACAGCGCTCCAAGGCTTTACCTAGCTCC
TAGCCAAGAAGCTGCCAAAGGACGCCTCGATCGGCAATTTTTTTCAGCTAGCACCGGTGGTATTTACATGTCCATGTACC
TAAAACCCAATGTCCCTTATACTGATATGCCTCCTTATACGATGATGGTTGCTTCCAGTATTGTCAAAGCTATCTCGCGA
TTAACAGGCATTGATACAGAAATCAAATGGGTAAACGACATCTATCTAGGTAACCATAAAGTTGCAGGGATTCTGACAGA
AGCCATTACTTCTGTTGAAACTGGTTTAATCACCGATGTCATTATTGGGGTTGGTCTCAACTTTTTTGTCACCGACTTCC
CACAAGCGATTGCTCAAAAGGCAGGTTCTCTCTTTACCGAAAAACCGACTATCACGCGCAATGACCTTATCATTGACATC
TGGAAATTATTCTTATCTATTCCTGTCAAGGACCATGTCAAAGTTTACAAAGAAAAATCACTTGTTCTTAATAAACAAGT
GACCTTTATAGAAAATAGTCAAGAAAAACGTGCTATTGCGATTGATCTCACAGATCAAGGACACCTCATTGTACAGTTTG
AAAATGGTGACCTTCAAACCTTGCGAAGCGGTGAAATCAGTCTTTCTTCTTGGGAGAATTAG

Upstream 100 bases:

>100_bases
AAGCTTCAACAAACAATAGTTTCTGTTGTTAGTAGCATTACACAAAAAACTAACTTTTCTTTTCCTTTGAAAATAAGATC
TTTTATAATATACTATACAT

Downstream 100 bases:

>100_bases
CCGTTTTTAAAATGGTTTTAGTCAGTTGGTTAATGGTATGCACTCTTCTTAAATCCCTAAAGAGCAACAAGCCCCAAAAT
AAGGCGAAGAGATAATTTCC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQGIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYT
PHSQSTQLDAKQGIEAHNSAPRLYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYTDMPPYTMMVASSIVKAISR
LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPQAIAQKAGSLFTEKPTITRNDLIIDI
WKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEKRAIAIDLTDQGHLIVQFENGDLQTLRSGEISLSSWEN

Sequences:

>Translated_313_residues
MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQGIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYT
PHSQSTQLDAKQGIEAHNSAPRLYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYTDMPPYTMMVASSIVKAISR
LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPQAIAQKAGSLFTEKPTITRNDLIIDI
WKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEKRAIAIDLTDQGHLIVQFENGDLQTLRSGEISLSSWEN
>Mature_313_residues
MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQGIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYT
PHSQSTQLDAKQGIEAHNSAPRLYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYTDMPPYTMMVASSIVKAISR
LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPQAIAQKAGSLFTEKPTITRNDLIIDI
WKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEKRAIAIDLTDQGHLIVQFENGDLQTLRSGEISLSSWEN

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=320, Percent_Identity=25, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34864; Mature: 34864

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQGIQIDSLKHKGYRMVQ
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
GDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPRLYLAPSQEAAKGRLDRQF
CCEECHHHHHHCCCCCEEECCCCCCCCCHHHHCCHHCCCCCEEEECCCCHHHHHHHHHHH
FSASTGGIYMSMYLKPNVPYTDMPPYTMMVASSIVKAISRLTGIDTEIKWVNDIYLGNHK
HCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCH
VAGILTEAITSVETGLITDVIIGVGLNFFVTDFPQAIAQKAGSLFTEKPTITRNDLIIDI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
WKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEKRAIAIDLTDQGHLIVQFENGDLQT
HHHHHHCCCHHHHHHHHHHHHEEECEEEEEECCCCCEEEEEEECCCCEEEEEECCCCEEE
LRSGEISLSSWEN
ECCCCEEECCCCC
>Mature Secondary Structure
MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQGIQIDSLKHKGYRMVQ
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
GDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPRLYLAPSQEAAKGRLDRQF
CCEECHHHHHHCCCCCEEECCCCCCCCCHHHHCCHHCCCCCEEEECCCCHHHHHHHHHHH
FSASTGGIYMSMYLKPNVPYTDMPPYTMMVASSIVKAISRLTGIDTEIKWVNDIYLGNHK
HCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCH
VAGILTEAITSVETGLITDVIIGVGLNFFVTDFPQAIAQKAGSLFTEKPTITRNDLIIDI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
WKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEKRAIAIDLTDQGHLIVQFENGDLQT
HHHHHHCCCHHHHHHHHHHHHEEECEEEEEECCCCCEEEEEEECCCCEEEEEECCCCEEE
LRSGEISLSSWEN
ECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]