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The map label for this gene is prfA [H]

Identifier: 94994408

GI number: 94994408

Start: 959076

End: 960155

Strand: Direct

Name: prfA [H]

Synonym: MGAS10750_Spy1012

Alternate gene names: 94994408

Gene position: 959076-960155 (Clockwise)

Preceding gene: 94994407

Following gene: 94994409

Centisome position: 49.51

GC content: 37.13

Gene sequence:

>1080_bases
ATGAATATTTATGATCAATTGCAGGCAGTTGAAGATCGTTATGAAGAATTAGGTGAGTTACTTAGTGATCCAGATGTTGT
TTCAGATACAAAGCGATTTATGGAATTATCACGTGAAGAAGCTAATACTCGAGAAACTGTCACAGCTTATAGAGAATATA
AACAAGTCATTCAAACCATTTCAGATGCTGAAGAAATGATCAAAGATGCCAGTGGTGATCCTGAATTAGAAGAAATGGCT
AAAGAAGAACTTAAAGAATCAAAAGCAGCTAAAGAAGAGTATGAAGAAAAATTAAAAATCCTTCTATTACCTAAAGATCC
TAACGATGATAAAAACATTATTCTAGAAATTCGTGGTGCTGCTGGTGGCGATGAAGCAGCGCTTTTTGCTGGTGATCTTT
TAACAATGTATCAAAAATATGCTGAAACTCAAGGATGGCGTTTTGAAGTTATGGAATCATCCGTTAATGGTGTTGGTGGT
ATTAAAGAAGTGGTTGCCATGGTTTCTGGTCAGTCAGTTTATTCTAAGCTTAAATATGAATCAGGAGCTCATCGTGTTCA
ACGTGTCCCGGTGACAGAGAGTCAGGGTCGTGTTCATACGTCAACTGCAACTGTTTTGGTAATGCCAGAAGTTGAAGAAG
TAGAATACGACATTGATCAAAAAGACCTTCGCGTCGATATTTACCACGCGTCTGGTGCTGGTGGTCAGAATGTCAATAAA
GTTGCTACTGCAGTTCGTATGGTACACATTCCTACAGGAATCAAAGTTGAAATGCAAGAAGAACGTACTCAGCAAAAAAA
CCGTGATAAAGCGATGAAAATCATTCGTGCTCGTGTTGCTGACCATTTTGCGCAAATTGCTCAAGATGAACAAGATGCTG
AACGTAAATCAACAGTTGGTACTGGTGATCGTTCAGAACGTATTCGTACTTATAATTTCCCACAAAATCGTGTAACAGAT
CATCGTATTGGTTTGACACTTCAAAAATTAGATACTATTTTATCTGGAAAAATGGATGAAGTTATTGATGCACTCGTTAT
GTATGATCAGACAAAGAAATTAGAATCTCTCAATAACTAA

Upstream 100 bases:

>100_bases
CAAATTGGTGGTAATGAAACATATATCCCAGTTTGTCGAAAACATTATTTTAATCCAGAGATTTAAAAGGTAATCGGTTT
TAAAGGAGATACAAAGAAAA

Downstream 100 bases:

>100_bases
TGAACTATGCTACATTAATCAGAACTTATGAAGATAAACTAGAACAAATAGATGAGGATCGCGAAAATCTCGCCTATGTT
TTTAGAGAAATCAAAGAATG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMA
KEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDQKDLRVDIYHASGAGGQNVNK
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN

Sequences:

>Translated_359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMA
KEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDQKDLRVDIYHASGAGGQNVNK
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN
>Mature_359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMA
KEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDQKDLRVDIYHASGAGGQNVNK
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=42.8082191780822, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=37.2492836676218, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=40.8163265306122, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=48.8888888888889, Blast_Score=350, Evalue=6e-98,
Organism=Escherichia coli, GI2367172, Length=349, Percent_Identity=35.243553008596, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=288, Percent_Identity=38.8888888888889, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=41.8238993710692, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19921226, Length=344, Percent_Identity=37.2093023255814, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40600; Mature: 40600

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTI
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGGIKEVVAMVSGQSVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHCCHHHHHHHHHC
SGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDQKDLRVDIYHASGAGGQNVNK
CCCHHHEECCCCCCCCEEEECCEEEEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHH
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVG
HHHHHHHEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
TGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTI
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGGIKEVVAMVSGQSVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHCCHHHHHHHHHC
SGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDQKDLRVDIYHASGAGGQNVNK
CCCHHHEECCCCCCCCEEEECCEEEEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHH
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVG
HHHHHHHEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
TGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA