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The map label for this gene is pucK [H]

Identifier: 94994404

GI number: 94994404

Start: 955811

End: 957094

Strand: Direct

Name: pucK [H]

Synonym: MGAS10750_Spy1008

Alternate gene names: 94994404

Gene position: 955811-957094 (Clockwise)

Preceding gene: 94994403

Following gene: 94994407

Centisome position: 49.34

GC content: 40.58

Gene sequence:

>1284_bases
ATGACACATTCAACAAAGCAGGAACATTCTCACTCGCAATCAGCTGTCCTTGGCTTACAGCATGTGCTTTCGATGTATGC
CGGATCTATTTTAGTACCAATTATGATCGCAGGTGCTTTAGGTTATTCCGCTAGAGAGTTGACCTACCTGATTTCGACAG
ATATTTTCATGTGTGGTGTGGCTACTTTCTTACAGTTAAAGTTAACCAAGCATACGGGAGTGGGATTACCAGTTGTTTTA
GGATGTGCTTTTCAATCAGTGGCACCTCTATCCATTATTGGAGCGCAACAAGGTTCAAGTGCCATGTTTGGAGCCCTCAT
TGCGTCAGGAATTTATGTCATTTTAGTGGCAGGTATTTTTTCTAAGATTGCTCGGTTCTTCCCACCAATTGTTACAGGAT
CGGTCATTACCGTTATTGGCTTAAGTCTCGTAGGTGTTGCTATGGGGAATATGGGAGATAACGTCAAAGAGCCTACGGCA
CAAAGCGTGATGTTATCATTGTTGACGATTGTCATTATTCTTTTAGTCCAAAAATTTACCAAAGGTTTTGTGAAATCCAT
TTCCATTTTGATTGGGCTTGTAGCGGGAACTTTGGTTTCAGCTATGATGGGATTAGTAGATACAACTCCTGTTGTAGAGG
CTTCTTGGATTCATGTGCCAACCCCATTTTATTTTGGCATGCCAACTTTTGACATCACTTCTATTGTGATGATGTGTATC
ATCGCAACGGTTTCTATGGTTGAATCAACAGGTGTCTACTTAGCGCTTTCAGATTTGACTAATGATCAATTAGATGAAAA
ACGTTTGCGCAATGGTTATCGCTCAGAAGGGATCGCAGTCTTTCTCGGTGGGTTATTTAATACCTTCCCTTACACTGGTT
TTTCTCAAAACGTTGGACTTGTCCAAATCTCAGGTATTAAAACCCGTCGTCCGATTTACTACGCAGCAGGTATCCTTGTG
GTCATTGGCCTACTCCCTAAATTTGGAGCAATGGCACAAATGATTCCAAGCCCAGTTCTTGGTGGAGCCATGCTTGTTCT
CTTTGGTATGGTTGCTCTTCAAGGAATGCAAATGCTTAATCGTGTTGATTTCCAAAAGAACGAGTATAATTTTATTATTG
CTGCTGTTTCCATTTCAGCAGGTTTAGGCTTTAATGGCACTAATCTTTTTGCCAGCTTACCAGAGACAGCTCAAATGTTC
TTAACGAATGGTATTGTGATTGCCACTTTGACATCTGTTGTCTTAAATTTAGTTTTAAATGGCAAAGACAAACAAGATGA
ATAA

Upstream 100 bases:

>100_bases
GTCGTCGATTGATTGAAGATATGGGCATTGAAGTGACCTCATTAGCACGTATTAAAAACTTTGAAAATGGTAATCTAAAC
TTCTTGGAGGCTGACGCATA

Downstream 100 bases:

>100_bases
TTAAAAAACGGCTAATTGAGCCGTTTTTTCTGTGATCAGTTTTGAGCAAACCGTTAACCCCTTTTAATGATAGTATGGTT
GAAAATAGTGTCTCAAACCA

Product: Xanthine permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVL
GCAFQSVAPLSIIGAQQGSSAMFGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
QSVMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFDITSIVMMCI
IATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILV
VIGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMF
LTNGIVIATLTSVVLNLVLNGKDKQDE

Sequences:

>Translated_427_residues
MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVL
GCAFQSVAPLSIIGAQQGSSAMFGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
QSVMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFDITSIVMMCI
IATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILV
VIGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMF
LTNGIVIATLTSVVLNLVLNGKDKQDE
>Mature_426_residues
THSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLG
CAFQSVAPLSIIGAQQGSSAMFGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTAQ
SVMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFDITSIVMMCII
ATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVV
IGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMFL
TNGIVIATLTSVVLNLVLNGKDKQDE

Specific function: Uptake of uric acid [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=437, Percent_Identity=25.4004576659039, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI40316845, Length=437, Percent_Identity=25.4004576659039, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI44680145, Length=422, Percent_Identity=25.8293838862559, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI44680143, Length=426, Percent_Identity=25.5868544600939, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI87082181, Length=436, Percent_Identity=38.5321100917431, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1790087, Length=447, Percent_Identity=29.082774049217, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788843, Length=419, Percent_Identity=28.4009546539379, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87081818, Length=442, Percent_Identity=28.2805429864253, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082178, Length=435, Percent_Identity=26.4367816091954, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17541904, Length=420, Percent_Identity=22.1428571428571, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558856, Length=155, Percent_Identity=27.741935483871, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17542262, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356175, Length=424, Percent_Identity=25.9433962264151, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45298; Mature: 45167

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCC
>Mature Secondary Structure 
THSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGV
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSSAMFGALIASGIYVILVAGIF
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTAQSVMLSLLTIVIILLVQKFT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFDITSIVMMCI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECEEECCCCCCCCCCCHHHHHHHHHHH
IATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGL
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCE
VQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLN
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMFLTNGIVIATLTSVVLNLVLN
HHCCCCCCCEEEEEEEEEECCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHC
GKDKQDE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]