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The map label for this gene is folC1 [H]

Identifier: 94994367

GI number: 94994367

Start: 920930

End: 922306

Strand: Direct

Name: folC1 [H]

Synonym: MGAS10750_Spy0971

Alternate gene names: 94994367

Gene position: 920930-922306 (Clockwise)

Preceding gene: 94994366

Following gene: 94994368

Centisome position: 47.54

GC content: 39.65

Gene sequence:

>1377_bases
GTGTTTGGTGAGGATAAGAAAAAAAAGGGTACTACTCTAGGTTATCTGCTGTCACTTTTTTTTATATATGGTATACTAAA
GATAGATGTGAGGTATGAAATGACTTATGAAGAGACTCTAGAATGGATTCATGATCACTTAGTTTTCGGCATCAAGCCAG
GCTTAAAACGTATGCTTTGGGTTCTTGGACAGTTAGGAAATCCCCAAAAAAATGTCAAGGGAGTGCATATCGTTGGAACT
AACGGAAAAGGTTCCACGGTCAATCATTTACAACACATCTTTACGACAGCTGGTTATGAAGTGGGGACCTTTACCTCTCC
TTATATTATGGATTTCAAAGAACGTATTAGCATAAATGGTCGAATGATTTCAGAAAAAGATTTGGTTATTGCTGCTAATC
GTATTCGTCCTTTGACAGAGCGTCTAGTTCGAGAGACTGATTTTGGAGAGGTAACTGAATTTGAAGTGATTACTTTGATC
ATGTTCCTCTATTTTGGTGATATGCATCCTGTAGATATTGCTATTATTGAAGCAGGCCTTGGCGGTCTTTATGATTCGAC
CAATGTTTTTCAAGCAATGGTTGTGGTCTGTCCCTCAATTGGTTTAGATCACCAAGCTATTTTGGGAGAGACTTACGCTG
ATATTGCCGCCCAAAAAGCTGGTGTTTTAGAAGGGGGCGAGACCCTTGTTTTTGCCGTTGAAAACCCGTCAGCTCGAGAG
GTTTTTTTAACAAAAGCTGAGCAGGTTGGTGCTTCAATCTGGGAATGGCAAGAGCAGTTTCAAATGGCTGAGAACGCATC
TGGCTATCGTTTTACTAGTCCTTTAGGAGTTATTTCAGATATTCACATCGCTATGCCAGGACACCACCAAGTGTCTAATG
CAGCTTTAGCTATTATGACTTGCTTAACCTTGAAAGACCGCTATCCTAGGCTAACTTCAGATCATATAAGGGAAGGTCTA
GCAAATAGTTTGTGGCTAGGCCGTACCGAATTATTAGCACCAAATCTAATGATTGATGGGGCTCATAACAATGAAAGCGT
TGCTGCTTTGGTAGCCGTCTTAAAAAATAACTACAATGATAAAAAGCTTCATATTTTGTTTGGTGCGATCGACACCAAGC
CTATTGCAGATATGTTGGTTGCTCTTGAGCAAATAGGTGACTTGCAGGTTACTAGCTTTCATTACCCTAACGCCTATCCA
TTGGAAAAATACCCAGAACGTTTTGGTAGGGTTGCTGATTTCAAAGATTTCTTGGCCCTGCGTAAGCATGCTAAAGCAGA
TGACTTTTTCGTGATTACAGGGTCACTATATTTTATTTCAGAAATTAGACGGTATTGGAAAAAACATATTGAAAAAAGCG
TTCTTTTAACCCATTAA

Upstream 100 bases:

>100_bases
AACACTTTAGGAGGTAGCAAAGACTATGTGGGACTGGTATTCTTATCACGTAAAAAAAGCAATGAAGATAAAAGTGTAGT
GTACTTTAGAACTTATTTGA

Downstream 100 bases:

>100_bases
AAAAGAGAAAATGAAAAGGAAAAAACTAATGAAAAGGGAAAGACTAATGTCTATTAATAAGGAAAAAGCAGAAGCAGCCA
TTTATCAGTTTTTAGAGGCT

Product: Folylpolyglutamate synthase / dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MFGEDKKKKGTTLGYLLSLFFIYGILKIDVRYEMTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGT
NGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRIRPLTERLVRETDFGEVTEFEVITLI
MFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMVVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSARE
VFLTKAEQVGASIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGL
ANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYP
LEKYPERFGRVADFKDFLALRKHAKADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH

Sequences:

>Translated_458_residues
MFGEDKKKKGTTLGYLLSLFFIYGILKIDVRYEMTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGT
NGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRIRPLTERLVRETDFGEVTEFEVITLI
MFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMVVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSARE
VFLTKAEQVGASIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGL
ANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYP
LEKYPERFGRVADFKDFLALRKHAKADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH
>Mature_458_residues
MFGEDKKKKGTTLGYLLSLFFIYGILKIDVRYEMTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGT
NGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRIRPLTERLVRETDFGEVTEFEVITLI
MFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMVVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSARE
VFLTKAEQVGASIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGL
ANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYP
LEKYPERFGRVADFKDFLALRKHAKADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=318, Percent_Identity=29.2452830188679, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI66932984, Length=318, Percent_Identity=29.2452830188679, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1788654, Length=399, Percent_Identity=25.31328320802, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=344, Percent_Identity=25.8720930232558, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17553150, Length=344, Percent_Identity=25.8720930232558, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17553148, Length=344, Percent_Identity=25.8720930232558, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323760, Length=446, Percent_Identity=26.9058295964126, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324815, Length=302, Percent_Identity=28.476821192053, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24641571, Length=335, Percent_Identity=26.865671641791, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24581568, Length=208, Percent_Identity=25.4807692307692, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 51502; Mature: 51502

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGEDKKKKGTTLGYLLSLFFIYGILKIDVRYEMTYEETLEWIHDHLVFGIKPGLKRMLW
CCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHH
VLGQLGNPQKNVKGVHIVGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISING
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
RMISEKDLVIAANRIRPLTERLVRETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGL
EEECCCCEEEEEHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GGLYDSTNVFQAMVVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSARE
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCH
VFLTKAEQVGASIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMT
HHEECHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCHHEEEEECCCCCCHHHHHHHHHH
CLTLKDRYPRLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYND
HHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCC
KKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLAL
CEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
RKHAKADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH
HHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFGEDKKKKGTTLGYLLSLFFIYGILKIDVRYEMTYEETLEWIHDHLVFGIKPGLKRMLW
CCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHH
VLGQLGNPQKNVKGVHIVGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISING
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
RMISEKDLVIAANRIRPLTERLVRETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGL
EEECCCCEEEEEHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GGLYDSTNVFQAMVVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSARE
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCH
VFLTKAEQVGASIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMT
HHEECHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCHHEEEEECCCCCCHHHHHHHHHH
CLTLKDRYPRLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYND
HHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCC
KKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLAL
CEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
RKHAKADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH
HHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]