Click here to switch to the map view.

The map label for this gene is lplB [H]

Identifier: 94994306

GI number: 94994306

Start: 864572

End: 865561

Strand: Direct

Name: lplB [H]

Synonym: MGAS10750_Spy0910

Alternate gene names: 94994306

Gene position: 864572-865561 (Clockwise)

Preceding gene: 94994303

Following gene: 94994309

Centisome position: 44.63

GC content: 37.68

Gene sequence:

>990_bases
ATGAAATACATCGTTAACAAAAGTCACAATCCTGCCTTTAACATCGCTTTGGAAGCCTATGCTTTTAGAGAACTAGTGGA
AGAAGACGAATTGTTTATCCTTTGGATCAATGAGCCAGCTATTATTATTGGCAAACATCAAAACACCATTCAAGAAATCA
ATAAAGAATACATTGATGAACATGGTATTCATGTTGTGCGACGTTTATCTGGTGGTGGTGCGGTTTATCATGATTTGAAT
AACTTGAACTACACTATCATTTCAAATAAAACAGCGGAAGGCGCATTTGATTTCAAAACCTTCTCACAACCTGTGATTGC
GACCTTAGCTGACTTGGGCGTAACAGCCAACTTTACAGGCCGTAATGATATTGAAATTGATGGCAAAAAAATCTGTGGTA
ATGCTCAAGCTTACTACAAAGGTCGCATGATGCACCATGGTTGCCTTTTGTTTGATGTGGATATGACCGTTCTTGGCGAT
GCTCTTAAGGTAAGTAAGGATAAGATTGAGTCTAAAGGAGTGAAGTCTGTTCGTGCCCGTGTGACCAATATTTTAAATGA
ATTACCAGAAAAAATTACCGTTGAGGAATTTTCAGATAAAATCTTGACCAAGATGAAAGAAACGTACCCAGACATGACAG
AATATGTCTTGTCGGAGGATGAGTTGGCTAAGATTGAACAATCTGCCAAAGAGCAATTTGGTAGCTGGGATTGGACCTAC
GGCAAAGCGCCAGAATATACGATTGAACGCAATGTTCGTTACCCTGCAGGTAAAATTTCAACCTTTGCAAATGTTGAAAA
TTCTATCATTAAAAACCTTAAAATCTATGGTGATTTCTTTGGAATCAAAGATGTGCAAGACATTGAGAACTTATTAATTG
GTTGTAAATATGAGTACCGTGATGTTTTTGAACGTTTAAAAACGATTGATACGACTCAGTACTTCTCACGCATGACCGTT
GAAGAAGTTGCTAAAGCTATTGTTTCTTAG

Upstream 100 bases:

>100_bases
AGGTACTGAAGATGTCATACAGATAGGACACTTGTCATTTAGGCTTGTTTTCATTATACTAGAGAAGTAGATTACATATT
TTTGAAAGGACTTTTTTCCT

Downstream 100 bases:

>100_bases
TGGTTTTAATCACTTAAGAAGCTCTGTCAGAAGAAAAAACTATAAAACTCCTAAGCGAACTATCGTGTCCTACTTAGGAG
TTTTTGTTTGATGACATGAA

Product: Lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIGKHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLN
NLNYTIISNKTAEGAFDFKTFSQPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLSEDELAKIEQSAKEQFGSWDWTY
GKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIYGDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTV
EEVAKAIVS

Sequences:

>Translated_329_residues
MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIGKHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLN
NLNYTIISNKTAEGAFDFKTFSQPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLSEDELAKIEQSAKEQFGSWDWTY
GKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIYGDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTV
EEVAKAIVS
>Mature_329_residues
MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIGKHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLN
NLNYTIISNKTAEGAFDFKTFSQPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLSEDELAKIEQSAKEQFGSWDWTY
GKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIYGDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTV
EEVAKAIVS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=298, Percent_Identity=29.8657718120805, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI21729884, Length=298, Percent_Identity=29.8657718120805, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI21729882, Length=298, Percent_Identity=29.8657718120805, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI21729880, Length=298, Percent_Identity=29.8657718120805, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI21729878, Length=298, Percent_Identity=29.8657718120805, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1790846, Length=305, Percent_Identity=31.4754098360656, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71981909, Length=249, Percent_Identity=31.3253012048193, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322415, Length=256, Percent_Identity=28.515625, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573464, Length=254, Percent_Identity=28.3464566929134, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37533; Mature: 37533

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIGKHQNTIQEINKEYIDE
CCEEECCCCCCEEEEEHHHHHHHHHHCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHH
HGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFSQPVIATLADLGVTANFTG
HHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHCCEEEECCC
RNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGDALKVSKDKIESKGVKSVRAR
CCCEEECCHHHCCCCHHHHHCCCEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
VTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLSEDELAKIEQSAKEQFGSWDWTY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCC
GKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIYGDFFGIKDVQDIENLLIGCKYEYR
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
DVFERLKTIDTTQYFSRMTVEEVAKAIVS
HHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIGKHQNTIQEINKEYIDE
CCEEECCCCCCEEEEEHHHHHHHHHHCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHH
HGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFSQPVIATLADLGVTANFTG
HHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHCCEEEECCC
RNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGDALKVSKDKIESKGVKSVRAR
CCCEEECCHHHCCCCHHHHHCCCEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
VTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLSEDELAKIEQSAKEQFGSWDWTY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCC
GKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIYGDFFGIKDVQDIENLLIGCKYEYR
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
DVFERLKTIDTTQYFSRMTVEEVAKAIVS
HHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]