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The map label for this gene is papS [H]

Identifier: 94994160

GI number: 94994160

Start: 737991

End: 739199

Strand: Direct

Name: papS [H]

Synonym: MGAS10750_Spy0764

Alternate gene names: 94994160

Gene position: 737991-739199 (Clockwise)

Preceding gene: 94994159

Following gene: 94994161

Centisome position: 38.1

GC content: 39.87

Gene sequence:

>1209_bases
ATGAAATTAATGACAATGCCTTCTGAGTTTCAGAAGGCTTTACCCATATTGACTAAAATAAAAGAAGCTGGTTATGAAGC
CTATTTTGTGGGAGGAAGCGTCAGAGATGTTTTACTCGAGCGTCCCATTCATGATGTAGATATTGCAACTAGTTCCTATC
CTGAAGAGACTAAAGCGATTTTTAACAGAACTGTCGATGTTGGCATTGAACATGGCACTGTTTTGGTTTTGGAAAATGGT
GGTGAATATGAAATTACAACCTTTAGGACAGAAGATGTCTATGTTGATTATCGCCGTCCTAGTCAGGTTTCTTTTGTGCG
CTCTTTAGAGGAAGACTTAAAACGAAGAGACTTTACCGTCAATGCCTTGGCCTTGGATGAAAATGGTCAAGTTATTGATA
AATTTAGAGGACTCATTGATTTGGAGCAAAAACGGTTACGAGCAGTGGGTAAAGCAGAGGAACGTTTTGAAGAAGATGCA
CTACGCATTATGAGAGGCTTTCGATTTGCGGCGAGTCTGGATTTTGACATTGAAGCTGCAACTTTTGAAGCTATGAGGAG
CCATTCACCTCTATTGGAAAAGATTTCTGTGGAACGTTCTTTTACTGAATTTGATAAATTATTGATGGCACCGCACTGGC
GAAAAGGGATTTCTGCCATGATTGCTTGTCAGGCTTATGACTATTTACCAGGCTTGAAGCAGCGGGAAGCAGGCTTGAAT
CATCTGATAGTTTCTTTGAAAGATAACTTTACCTTTAGTGATCACCACCAAGCTTGGGCTTATGTGATGATTAGTTTAGC
CATTGAGGATCCTAAAAGCTTCCTAAAAGCATGGAAGACTTCAAATGATTTTCAGCGTTATGTCACAAAGTTGATTGCTC
TTTATAGAATTCGTCAGGAAAGATCTTTTGAAAAATTGGATATTTACCAGTACGGCAAGGAAATGGCAAGTCTAGTGGAA
GGCCTCCGAAAAGCTCAAAGCTTATCTGTTGACATGGACCACATTGAAGCTTTAGATCAAGCGCTAGCCATTCATAACAA
ACACGATATCGTCCTTAATGGTTCTCACCTGATTAAGGATTTTGGGATGGAACCAGGTCCTCAGCTAGGGCTTATGCTTG
AGAAAGTTGAATTAGCGATTGTAGAGGGATGCCTAGATAATGATTTCACTACAATTGAAGCATTTGTTAGAGAGGAATTA
GCAACATGA

Upstream 100 bases:

>100_bases
TTGCAAGAATCACTAAAACAATAACAAAATGTGATAGAACCCAAGTAGCTTAATCACACTTTAGATTGGGCTAGTTGGAT
GATAGGTTAGAAAACAACGT

Downstream 100 bases:

>100_bases
GCCATTTTTTGGTGGAGAAATTGACAAAAACGGTGGGAGATAAGACCGTCTTTCAGGATATTTCCTTTATTATTCATGAC
TTTGACCGTATTGGGATTAT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQREAGLN
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVE
GLRKAQSLSVDMDHIEALDQALAIHNKHDIVLNGSHLIKDFGMEPGPQLGLMLEKVELAIVEGCLDNDFTTIEAFVREEL
AT

Sequences:

>Translated_402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQREAGLN
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVE
GLRKAQSLSVDMDHIEALDQALAIHNKHDIVLNGSHLIKDFGMEPGPQLGLMLEKVELAIVEGCLDNDFTTIEAFVREEL
AT
>Mature_402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQREAGLN
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVE
GLRKAQSLSVDMDHIEALDQALAIHNKHDIVLNGSHLIKDFGMEPGPQLGLMLEKVELAIVEGCLDNDFTTIEAFVREEL
AT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=224, Percent_Identity=36.1607142857143, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI87081691, Length=211, Percent_Identity=35.0710900473934, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789436, Length=195, Percent_Identity=38.4615384615385, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=228, Percent_Identity=35.0877192982456, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281360112, Length=386, Percent_Identity=30.8290155440415, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21357337, Length=386, Percent_Identity=30.8290155440415, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46019; Mature: 46019

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTV
HHHHHEEEECCCEEEEEECCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCEE
NALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHH
TFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQREAGLN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHH
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQE
HEEEEEECCCEECCCHHHHHHHEEEHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDHIEALDQALAIHNKHDIVLNGSHLIKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHH
FGMEPGPQLGLMLEKVELAIVEGCLDNDFTTIEAFVREELAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQVSFVRSLEEDLKRRDFTV
HHHHHEEEECCCEEEEEECCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCEE
NALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHH
TFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQREAGLN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHH
HLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQE
HEEEEEECCCEECCCHHHHHHHEEEHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDHIEALDQALAIHNKHDIVLNGSHLIKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHH
FGMEPGPQLGLMLEKVELAIVEGCLDNDFTTIEAFVREELAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]