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The map label for this gene is rmlD [H]

Identifier: 94994085

GI number: 94994085

Start: 665097

End: 666011

Strand: Direct

Name: rmlD [H]

Synonym: MGAS10750_Spy0689

Alternate gene names: 94994085

Gene position: 665097-666011 (Clockwise)

Preceding gene: 94994084

Following gene: 94994086

Centisome position: 34.33

GC content: 40.11

Gene sequence:

>915_bases
ATGTTAGAGAACACTAAGATACTGAGAAAAGTGTTCTACCTATGGCAAAAAGGAGAACTGATGATTTTAATTACAGGAAG
CAATGGTCAATTAGGGACAGAACTTCGATACTTATTAGATGAACGAGGTGTTGATTATGTAGCCGTTGATGTCGCAGAAA
TGGACATCACAAATGAAGACAAAGTCGAAGCTGTTTTTGCGCAGGTCAAGCCAACATTGGTCTATCATTGTGCTGCTTAT
ACGGCAGTTGATGCAGCAGAAGACGAAGGAAAAGCTTTGAATGAGGCTATTAATGTAACAGGCTCAGAAAATATTGCCAA
GGCTTGTGGAAAATATGGGGCTACCCTTGTTTACATTTCAACAGACTACGTATTTGATGGCAATAAACCAGTCGGTCAAG
AATGGGTAGAAACAGATTGTCCAGACCCTAAGACAGAATATGGCCGTACAAAACGTTTAGGAGAACTAGCTGTTGAACGA
TACGCAGAGCATTTTTATATTATCCGTACTGCTTGGGTATTTGGAAATTATGGTAAAAACTTTGTCTTTACCATGGAGCA
GTTAGCGGAAAATCACTCCCGTTTGACTGTTGTCAATGACCAACACGGTCGGCCAACGTGGACAAGAACCTTGGCAGAAT
TCATGTGTTATTTGACAGAGAATCAAAAAGCCTTTGGTTACTATCACTTGTCAAATGATGCTAAGGAAGACACCACTTGG
TATGATTTTGCCAAAGAAATCTTAAAAGACAAGGCTGTTGAAGTGGTGCCAGTTGATTCATCAGCTTTCCCAGCAAAAGC
CAAACGTCCTTTAAATTCAACCATGAATCTTGATAAGGCAAAAGCAACAGGGTTTGTTATTCCAACATGGCAAGAAGCCT
TGAAGGCATTTTACCAACAAGGACTGAAAAAGTAA

Upstream 100 bases:

>100_bases
GGATTGCGCTGGGTATTCGCTAATCATTGATAGTAAAGGGATGAAATATTCCCTTTTTTGATGTAGAATAAAAGGAAATA
CAGGTGAAAGCCTTTGGTTA

Downstream 100 bases:

>100_bases
GTATTCTAAAAAGAGTTGGCTTTCTAAGGCCAACTCTTTTTCATGGGTTTAAGGTCGCATTAGCCTTCTAATTGTGGTAT
AATATCACTGATTATATTTT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLENTKILRKVFYLWQKGELMILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAY
TAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDCPDPKTEYGRTKRLGELAVER
YAEHFYIIRTAWVFGNYGKNFVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTW
YDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKAFYQQGLKK

Sequences:

>Translated_304_residues
MLENTKILRKVFYLWQKGELMILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAY
TAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDCPDPKTEYGRTKRLGELAVER
YAEHFYIIRTAWVFGNYGKNFVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTW
YDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKAFYQQGLKK
>Mature_304_residues
MLENTKILRKVFYLWQKGELMILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEAVFAQVKPTLVYHCAAY
TAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTDYVFDGNKPVGQEWVETDCPDPKTEYGRTKRLGELAVER
YAEHFYIIRTAWVFGNYGKNFVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTW
YDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKAFYQQGLKK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=284, Percent_Identity=26.056338028169, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI33519455, Length=284, Percent_Identity=26.056338028169, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788352, Length=293, Percent_Identity=36.1774744027304, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 34447; Mature: 34447

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENTKILRKVFYLWQKGELMILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNED
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCH
KVEAVFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYIS
HHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEE
TDYVFDGNKPVGQEWVETDCPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
EEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCC
FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTW
CEEEHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHH
YDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKAFYQQ
HHHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCCCCCCHHHCCEEEECHHHHHHHHHHH
GLKK
HCCC
>Mature Secondary Structure
MLENTKILRKVFYLWQKGELMILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNED
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCH
KVEAVFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYIS
HHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEE
TDYVFDGNKPVGQEWVETDCPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
EEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCC
FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDTTW
CEEEHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHH
YDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKAFYQQ
HHHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCCCCCCHHHCCEEEECHHHHHHHHHHH
GLKK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]