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The map label for this gene is kgdA [H]

Identifier: 94993942

GI number: 94993942

Start: 528988

End: 529641

Strand: Direct

Name: kgdA [H]

Synonym: MGAS10750_Spy0546

Alternate gene names: 94993942

Gene position: 528988-529641 (Clockwise)

Preceding gene: 94993941

Following gene: 94993943

Centisome position: 27.31

GC content: 42.05

Gene sequence:

>654_bases
ATGTCCAGAGGTGATGATATGGGAAAAATAGAAATCTTAACCAAACTTAAGGCTAATCGGCTTGTCCTGGTTGTACGAGG
AGAGTCTAGTGAGGAAGCTTTAGCTTGCAGCCTGGCCAGTATTGAAGGTGGCATTAAGACAATTGAAGTCACCTATACTA
ACCCCTTTGCTAGTGAGGTTATCGGTCAATTAGCTGAACGCTTCAAAGAAGATCCTGAGGTATTGATAGGTGCTGGGACA
GTTTTGGATGATGTCACTGCCCGCCAAGCTATTTTAGCAGGAGCACAGCTTATTGTTGGTCCTAATTTCAATCGAGCAGT
GGCTTTGATTTGTCATCGTTATAGCATACCTTATTTACCAGGTTGTATGACGGTTAATGAAGTGGTTACAGCATTAGAAT
CAGGGGTTGATATGGTCAAAATTTTTCCTGGTTCTACTGTAGGGATGTCCTTTATTCGGGCAATCAAATCTCCTTTGCCA
CAAGTAGAGGTTATGGTTACAGGCGGGGTTTCTTCAGATAATCTCAAAGACTGGCTAGCTGCTGGAGTAGATGTTCTAGG
CATAGGAGGCGAGTTTAATCAGTTAGCGAGCCAAAAACAATATAACCTAATCACTAAGAAAGCCGCTCATTATATCAAAA
GTTTGCGACAATAA

Upstream 100 bases:

>100_bases
GATTTTGCTGTGGCAACAGCTAGCCTAAAATGTACAGTAGCTGAAGATCAGCTCTATCACAGCGCCCAACAAGTAGAAGC
AGTCTTAGCTAATCAACGAG

Downstream 100 bases:

>100_bases
TCTAAAAAACGTTTTTACCTTGGCTTTAGTGCTAGTAAAAACGTTTTTTATCATTCATTCAATTAATATGCTTCTGCTTA
TGTTTTTTCAGATAGTATAG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSRGDDMGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEVTYTNPFASEVIGQLAERFKEDPEVLIGAGT
VLDDVTARQAILAGAQLIVGPNFNRAVALICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLP
QVEVMVTGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ

Sequences:

>Translated_217_residues
MSRGDDMGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEVTYTNPFASEVIGQLAERFKEDPEVLIGAGT
VLDDVTARQAILAGAQLIVGPNFNRAVALICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLP
QVEVMVTGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ
>Mature_216_residues
SRGDDMGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEVTYTNPFASEVIGQLAERFKEDPEVLIGAGTV
LDDVTARQAILAGAQLIVGPNFNRAVALICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQ
VEVMVTGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=209, Percent_Identity=34.4497607655502, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788156, Length=190, Percent_Identity=32.6315789473684, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23238; Mature: 23107

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGDDMGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEVTYTNPFASEV
CCCCCCCCHHHHEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHH
IGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQLIVGPNFNRAVALICHRYSIPYLP
HHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCC
GCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMVTGGVSSDNLKDWLA
CCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
AGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRGDDMGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEVTYTNPFASEV
CCCCCCCHHHHEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHH
IGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQLIVGPNFNRAVALICHRYSIPYLP
HHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCC
GCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMVTGGVSSDNLKDWLA
CCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
AGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]