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The map label for this gene is cofE [H]

Identifier: 94993859

GI number: 94993859

Start: 453690

End: 454448

Strand: Direct

Name: cofE [H]

Synonym: MGAS10750_Spy0463

Alternate gene names: 94993859

Gene position: 453690-454448 (Clockwise)

Preceding gene: 94993858

Following gene: 94993860

Centisome position: 23.42

GC content: 33.33

Gene sequence:

>759_bases
ATGAGTATATTAATGGAGGCTAAAATGAATTTGAGTATATTTGGTTTAAAGAATATTCCTTATTTGAAAGAAGGGGATAG
TATAGAAAAGCTTATTGAAGAAAGCATTAAAACTTCAGAATTTTTTATCGAAGACAATGATGTGCTTTGCATAGCTTCTA
AAGTTGTATCTGTCGCAGAAGGCCAGGTGATGTCTTTAAACGAGATACAAGTGAGTGATGTAGCAAAAGAAATCCACAGG
AATATTCCTCGTAAAGATCCTAGAATAATTGAAATTATGCTAAATTTAGTCAATAGAGATCTGTCACGTTTAGATATCAA
AAAAAATTATATTGGTTGTCGCTTGGAAAATGGTTTAAAGTTGACAAGTGGTGGAATCGATAGAAAATCAGTAGATGAAG
TATTTCTTTTACCTAATAATCCGGATGCTTCTGCAAAAAGAATTTCGGAATATCTTAAAAAATCACTTGGAAAAAATGTT
GCAGTTGTTATAACAGATAGTGATGGTAGAGAGGATAAAAGAGGTGCTACTCAAGTAGCAATTGGTATTTATGGTATTCA
TCCTTTGAGAAAAACAGAAGTAATAGATTCACAAGGAGAGACAATAAAATTCCAAGAAGAGACTTTGTGTGATATGATTG
CAGCTTGTGCTGGCTTAGTAATGGGGCAACGTGGAACTGGAATTCCAGCTGTCCTCATTAAGGGCTTGGATTATAAATGG
TTTGAAAATACTAGTATTAAGGAAGCGTTAAATGAGTAG

Upstream 100 bases:

>100_bases
ATCCATGAACATTTTATAGACTTAATTTTTAGTTTAGAAGTACATGAAAAAGAAATAGACAAAAAGGCAGAAGTAGTGAG
GAAGTCTTATCTGACACTAA

Downstream 100 bases:

>100_bases
AAATCAGGACTGTAAGAATATTCTAATCATTGGTTTTGGAAAAATTGGGAAAATTAAAGCGTACAAGTGGCTATCACGCG
GATATGAGGTATATGTCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSILMEAKMNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSVAEGQVMSLNEIQVSDVAKEIHR
NIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNV
AVVITDSDGREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIKGLDYKW
FENTSIKEALNE

Sequences:

>Translated_252_residues
MSILMEAKMNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSVAEGQVMSLNEIQVSDVAKEIHR
NIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNV
AVVITDSDGREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIKGLDYKW
FENTSIKEALNE
>Mature_251_residues
SILMEAKMNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSVAEGQVMSLNEIQVSDVAKEIHRN
IPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVA
VVITDSDGREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIKGLDYKWF
ENTSIKEALNE

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 28000; Mature: 27869

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILMEAKMNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSVAE
CCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHC
GQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLK
CCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHCEEEEEECCCEE
LTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSDGREDKRGATQVA
ECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEE
IGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIKGLDYKW
EEEEECCCCCHHHEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEE
FENTSIKEALNE
CCCCCHHHHHCC
>Mature Secondary Structure 
SILMEAKMNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSVAE
CEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHC
GQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLK
CCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHCEEEEEECCCEE
LTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSDGREDKRGATQVA
ECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEE
IGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIKGLDYKW
EEEEECCCCCHHHEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEE
FENTSIKEALNE
CCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]