Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 94993836

GI number: 94993836

Start: 429651

End: 431594

Strand: Direct

Name: thrS [H]

Synonym: MGAS10750_Spy0440

Alternate gene names: 94993836

Gene position: 429651-431594 (Clockwise)

Preceding gene: 94993835

Following gene: 94993837

Centisome position: 22.18

GC content: 43.16

Gene sequence:

>1944_bases
ATGATTAAGATTACTTTTCCAGATGGCGCCGTGCGTGAATTTGAATCTGGCGTAACAACGTTTGACATTGCTGAGTCCAT
CAGCAAATCATTGGCTAAAAAAGCTTTAGCTGGTAAGTTCAATGATCAACTCATTGACACCACTCGTGCCATTGAAGAAG
ATGGCAGTATTGAAATCGTGACACCTGATCACAAAGATGCTTATGAGGTGCTTCGTCACTCTGCAGCCCATTTATTTGCC
CAAGCTGCAAAACGCTTGTTCCCAAATCTTCATTTGGGTGTGGGCCCTGCCATTGCAGAAGGCTTTTACTATGATACAGA
TAATGCAGAAGGCCAAATTTCAAACGAAGATTTGCCACGTATCGAAGCTGAAATGCAAAAAATCGTGACAGAAAATTACC
CATGTATCCGTGAAGAAGTGACCAAAGAAGAAGCTTTGGAATTGTTCAAAGATGATCCTTACAAGGTAGAATTGATTAAT
GAGCATGCTGGTGCTGGCTTGACGGTTTACCGTCAAGGTGAATTTGTGGATCTTTGCCGTGGCCCTCATGTGCCATCAAC
AGGCCGTATCCAAGTCTTCCACTTGCTCAATGTGGCGGGTGCTTACTGGCGTGGTAACAGCGACAATAACATGATGCAAC
GTATCTATGGAACAGCTTGGTTTGATAAAAAAGACCTCAAAGCTTACTTGACTCGTCTAGAAGAAGCCAAAGAACGTGAT
CACCGTAAGCTTGGTAAAGAGCTTGACTTGTTCATGATTAGCCAAGAAGTTGGTCAAGGCTTGCCGTTTTGGTTACCAGA
TGGAGCGACTATTCGTCGTACACTTGAGCGCTACATCACAGATAAAGAATTAGCATCAGGCTACCAACACGTTTACACAC
CACCACTTGCTTCTGTAGAATTGTACAAGACATCAGGTCATTGGGATCATTATCAAGAAGATATGTTCCCTGTAATGGAT
ATGGGAGATGGTGAGGAGTTTGTTCTTCGTCCAATGAACTGCCCACACCACATTCAAGTGTATAAAAATCATGTGCGTTC
TTATCGTGAATTGCCTATTCGTATTGCCGAGCTTGGCATGATGCACCGTTACGAAAAATCAGGTGCCCTATCAGGACTTC
AACGGGTACGTGAAATGACCTTGAATGATGGCCACATTTTCGTAACACCAGAGCAAATCCAGGAAGAATTTCGACGTGCC
CTTCAATTAATCATTGATGTTTATGCTGACTTCAACTTGACTGATTATCGTTTTCGTTTGTCATACCGAGACCCTAACGA
TACTCATAAATATTATGACAATGATGAGATGTGGGAAAATGCTCAAAGTATGCTTAAAGCTGCACTTGATGAGATGGGTG
TAGATTACTTTGAAGCAGAAGGTGAAGCAGCCTTTTATGGGCCTAAGCTTGATATTCAGGTGAAAACAGCTCTTGGTAAC
GAAGAAACCCTATCAACCATTCAGTTGGACTTCTTGTTGCCAGAACGCTTTGACCTTAAATACATTGGTGCAGATGGCGA
AGAACACCGTCCAGTCATGATTCACCGTGGTGTTATCTCAACTATGGAACGTTTCACTGCTATCCTTATTGAAACTTACA
AGGGTGCTTTCCCAACTTGGTTGGCACCTCACCAAGTAACCGTGATTCCAATTTCAAATGAAGCCCACATTGACTATGCT
TGGGAAGTAGCAAAAACCCTTCGTGACCGCGGTGTTCGTGCAGATGTCGATGACCGTAATGAAAAAATGCAGTACAAGAT
TCGTGCGTCTCAAACCAGCAAAATTCCTTACCAATTAATTGTTGGGGACAAAGAAATGGAAGACAAATCAGTCAATGTTC
GCCGCTATGGTAGCAAGGCGACTCACACCGAATCTGTGGAAGAATTTGTGGAAAACATCTTGGCAGACATTGCTCGCAAG
TCACGTCCAGATGCACAAGCTTAA

Upstream 100 bases:

>100_bases
CTACGTGGTGGTGTTGAGCTATTCACTAACCTCTTGTCCCTAATTTGTCTTACTATCCATGTTTTAGTGGAAATCTTAAC
TGACTTAAAGGAGAAAGCAA

Downstream 100 bases:

>100_bases
TCAATGGCAAAAAGAGGTCCTTATGGGCTTCTTTTTCTTTCTTAAAAAATTAAGCAAACGGTTGCACCGTCTTTTTCATG
GTGTTATAATAAATTGTTTA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQLIDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFA
QAAKRLFPNLHLGVGPAIAEGFYYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGTAWFDKKDLKAYLTRLEEAKERD
HRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMD
MGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFRRA
LQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGN
EETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYA
WEVAKTLRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTESVEEFVENILADIARK
SRPDAQA

Sequences:

>Translated_647_residues
MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQLIDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFA
QAAKRLFPNLHLGVGPAIAEGFYYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGTAWFDKKDLKAYLTRLEEAKERD
HRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMD
MGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFRRA
LQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGN
EETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYA
WEVAKTLRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTESVEEFVENILADIARK
SRPDAQA
>Mature_647_residues
MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQLIDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFA
QAAKRLFPNLHLGVGPAIAEGFYYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGTAWFDKKDLKAYLTRLEEAKERD
HRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMD
MGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFRRA
LQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGN
EETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYA
WEVAKTLRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTESVEEFVENILADIARK
SRPDAQA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=636, Percent_Identity=39.4654088050315, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI38570111, Length=623, Percent_Identity=40.1284109149278, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI20070344, Length=664, Percent_Identity=35.6927710843374, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1788013, Length=647, Percent_Identity=41.7310664605873, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1786392, Length=169, Percent_Identity=24.8520710059172, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=616, Percent_Identity=40.2597402597403, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984192, Length=616, Percent_Identity=40.2597402597403, Blast_Score=456, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=658, Percent_Identity=38.9057750759878, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322654, Length=415, Percent_Identity=36.3855421686747, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24583839, Length=635, Percent_Identity=39.6850393700787, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24583842, Length=635, Percent_Identity=39.6850393700787, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI28574141, Length=635, Percent_Identity=39.6850393700787, Blast_Score=468, Evalue=1e-132,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 74304; Mature: 74304

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQLIDTTRAIEEDGSIEIV
CEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEE
TPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGFYYDTDNAEGQISNEDLPR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCCCCCCCCCCCHH
IEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELINEHAGAGLTVYRQGEFVDLCR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCHHHHC
GPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGTAWFDKKDLKAYLTRLEEAKERD
CCCCCCCCCEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVE
HHHHCCHHHHEEEEHHHCCCCCEECCCCHHHHHHHHHHHCHHHHHCCCCEECCCCCCCEE
LYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGM
EEECCCCCCHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
MHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFRRALQLIIDVYADFNLTDYRFRL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGN
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCEEEEEEEECCCC
EETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFPTW
CHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCC
LAPHQVTVIPISNEAHIDYAWEVAKTLRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLI
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEE
VGDKEMEDKSVNVRRYGSKATHTESVEEFVENILADIARKSRPDAQA
ECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQLIDTTRAIEEDGSIEIV
CEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEE
TPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGFYYDTDNAEGQISNEDLPR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCCCCCCCCCCCHH
IEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELINEHAGAGLTVYRQGEFVDLCR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCHHHHC
GPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGTAWFDKKDLKAYLTRLEEAKERD
CCCCCCCCCEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVE
HHHHCCHHHHEEEEHHHCCCCCEECCCCHHHHHHHHHHHCHHHHHCCCCEECCCCCCCEE
LYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGM
EEECCCCCCHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
MHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFRRALQLIIDVYADFNLTDYRFRL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGN
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCEEEEEEEECCCC
EETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFPTW
CHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCC
LAPHQVTVIPISNEAHIDYAWEVAKTLRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLI
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEE
VGDKEMEDKSVNVRRYGSKATHTESVEEFVENILADIARKSRPDAQA
ECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA