Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 94993566

GI number: 94993566

Start: 185121

End: 186263

Strand: Direct

Name: tgt [H]

Synonym: MGAS10750_Spy0170

Alternate gene names: 94993566

Gene position: 185121-186263 (Clockwise)

Preceding gene: 94993565

Following gene: 94993567

Centisome position: 9.56

GC content: 43.31

Gene sequence:

>1143_bases
ATGACAGATTATCCGATTAAGTATCGCCTTATCAAAACAGAAAAGCACACCGGTGCACGCTTGGGCGAAATTATCACCCC
TCACGGGACCTTTCCGACACCAATGTTTATGCCAGTGGGAACTCAGGCTACTGTTAAAACACAGTCTCCAGAAGAGTTAA
AAGCAATTGGATCAGGGATTATTTTATCAAACACTTATCACCTCTGGTTGCGTCCAGGAGATGAGTTGATTGCTCGCTCT
GGTGGTCTGCACAAGTTTATGAACTGGGATCAGCCAATCTTAACCGATTCGGGTGGTTTTCAAGTTTACTCTTTAGCCGA
TTCACGAAACATCACTGAAGAAGGAGTGACTTTCAAAAATCACTTGAATGGCTCAAAAATGTTTCTATCACCCGAAAAAG
CCATTTCCATTCAGAATAACCTTGGCTCAGACATCATGATGAGTTTTGACGAGTGCCCGCAATTTTACCAACCTTATGAT
TACGTGAAAAAATCCATTGAACGTACTAGCCGTTGGGCAGAACGTGGCTTAAAAGCCCATCGTCGTCCTCATGACCAAGG
ATTATTTGGAATTGTGCAAGGAGCTGGATTTGAGGACCTTCGTCGTCAGTCTGCGGCAGATTTGGTGGCTATGGATTTCC
CAGGTTATTCTATTGGTGGTTTAGCTGTTGGGGAGAGCCATGAAGAAATGAATGCCGTCCTTGACTTTACGACTCCCCTC
TTACCAGAAAATAAACCTCGTTACCTGATGGGAGTGGGAGCACCAGATAGCTTGATTGATGGTGTGATTCGTGGTGTCGA
TATGTTTGACTGTGTCTTGCCAACGCGTATCGCTCGTAATGGGACCTGCATGACTAGTGAGGGGCGTCTTGTGGTTAAAA
ATGCCAAATTCGCTGAAGATTTTACCCCACTTGATCATGACTGTGACTGCTACACTTGTCAAAACTATAGCCGTGCCTAC
ATTCGTCACTTGCTAAAAGCGGACGAAACTTTTGGTATTCGTTTGACAAGCTACCACAACCTTTATTTCTTGGTTAACTT
GATGAAAAAAGTACGTCAGGCTATTATGGACGATAACTTACTTGAATTCCGCCAAGATTTTCTTGAACGGTATGGTTATA
ATAAATCAAACCGTAATTTCTAA

Upstream 100 bases:

>100_bases
CTAGAACAACATGTCATCTTGAAGCCCTTCATTATGATGGAAACTCAGGCAGATCAAAAAAGCTCAAGACTGTTTATCTT
GATAGCAAGGAAGAAAGCAC

Downstream 100 bases:

>100_bases
TTAAAAATGCTATGATCCTGGAACTGAGGGCGATCTATCGTCTTTGGTTTCAGTTTTTTTGTATAGACGGATGTCCTATT
GCTTTCAACTGATAGGTCAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARS
GGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSIGGLAVGESHEEMNAVLDFTTPL
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAY
IRHLLKADETFGIRLTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF

Sequences:

>Translated_380_residues
MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARS
GGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSIGGLAVGESHEEMNAVLDFTTPL
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAY
IRHLLKADETFGIRLTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF
>Mature_379_residues
TDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSG
GLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYDY
VKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSIGGLAVGESHEEMNAVLDFTTPLL
PENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAYI
RHLLKADETFGIRLTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=45.0549450549451, Blast_Score=336, Evalue=3e-92,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=50.8152173913043, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=45.1977401129944, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=207, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=42.7792915531335, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI21355311, Length=222, Percent_Identity=26.5765765765766, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43071; Mature: 42939

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKAIGSGI
CCCCCEEEEEEECCCCCCCHHCCEECCCCCCCCCEEEECCCCEEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKN
EEECEEEEEECCCHHHHHCCCCCHHHCCCCCCEEECCCCEEEEEECCCCCCCHHCCCHHH
HLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYDYVKKSIERTSRWAERGLKAH
CCCCCEEEECHHHHEEECCCCCHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSIGGLAVGESHEEMNAVLDFTTPL
CCCCCCCCEEEECCCCHHHHHHHHHHCEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCC
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFAED
CCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHHC
FTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIRLTSYHNLYFLVNLMKKVRQAIMDDNL
CCCCCCCCCCEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHH
LEFRQDFLERYGYNKSNRNF
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKAIGSGI
CCCCEEEEEEECCCCCCCHHCCEECCCCCCCCCEEEECCCCEEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKN
EEECEEEEEECCCHHHHHCCCCCHHHCCCCCCEEECCCCEEEEEECCCCCCCHHCCCHHH
HLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYDYVKKSIERTSRWAERGLKAH
CCCCCEEEECHHHHEEECCCCCHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSIGGLAVGESHEEMNAVLDFTTPL
CCCCCCCCEEEECCCCHHHHHHHHHHCEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCC
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFAED
CCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHHC
FTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIRLTSYHNLYFLVNLMKKVRQAIMDDNL
CCCCCCCCCCEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHH
LEFRQDFLERYGYNKSNRNF
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA