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The map label for this gene is gidA [H]

Identifier: 94986434

GI number: 94986434

Start: 2456575

End: 2458419

Strand: Reverse

Name: gidA [H]

Synonym: Dgeo_2337

Alternate gene names: 94986434

Gene position: 2458419-2456575 (Counterclockwise)

Preceding gene: 94986443

Following gene: 94986433

Centisome position: 99.64

GC content: 66.07

Gene sequence:

>1845_bases
GTGCTCGCTGCTTCCCCACTGCTACACTTCGGCGATATGCGCGGCTGGAACGTGATGGTGATTGGCGGCGGACACGCGGG
CCTGGAGGCTGCTTGGGCCGCCGCGAAGTTTGCCCGGGTGGCGGTGCTGGTGAGCAATCCCGCCACGGTTGGCCGGATGC
CCTGTAATCCAGCGGTGGGCGGTCCCGGCAAAAGCCAGCTGGTGTTCGAGGTGCAGGCCTTGGGCGGCCTGATGGGCCGT
TTGGCCGACGACACAGCAATCCACACCCGCGTGCTCAACGCGAGCAAGGGGCCTGCGGTGCAGTCGCTGCGTGTACAAAA
CGAGCGCGACGCTTATGCCGAACGCGCCCAGGAGATCCTGTTGGGCCATTCTGGAATCGACGTGGTGCGAGGCGAGGCCG
CCGACCTCGAGCCTGACGGGTGCGGTGGCTGGTTCGTGGTGACGACCGATGGCCGCCGCTTTCACGCCCGCAGCGTGGTG
GTCGCGGCAGGAACCTTTATGCGTGGCGTGACCTGGTATGGTCGCTATTCCCGGCCCGAAGGTCGTCAGGGGGAGCCCCC
TTCCCGCTTTCTCAGCGCACCGCTGGCGCGTGCCGGCCACCGTCTCAAGCGCTACAAGACCGGCACGCCACCCCGTGTGC
GGGCTGATTCGGTGCGCTTTGCCGACCTGTTGGAGATTCCGGCCGATCCGCAGCCGCGCGGGTTCACTGGGCGTCCTGGC
CCGCGTGCCGCCGAGTCGCCCACTTGGCAGACCCACACCACGCCGGAAACTCACCGCCTGATCCAAGAGAACCTGCACGA
GTCTCCCATGTATGCCGGCGACATCGCGGGGCTGGGACCGCGCTACTGCCCCAGCATCGAGGACAAGGTGGTTCGCTTTG
CCCATCATGACCGCCACCTGCTGTTCGTGGAGCCGGACGGCGTGCAGACCAGCGAGGTGTATCTCCAGGGATTTAGCTCC
AGCTTGCCTCCCGCACTCCAAGATCGCCTGGTTCGCAGCCTGCCGGGCTTTGAGGCGGCGGTGATCCAGCGTTACGCCTA
TGCCGTGGAGTACGACGTGGTGGACTCCACCGAACTCACCCTCAACCTGGAATCCCGCCTGCTGCCGGGAATTTTCACCG
CCGGGCAGATCAACGGCACCAGCGGGTATGAGGAGGCCGCAGCTCAGGGTCTGGTCGCCGGCACCGCTGCCGCGCGCCGA
GCGCTGGAACTCGAGGAGCTTCAGATTTCACGTGAAACGAGCTATCTGGGGGTGCTGCTCGACGACCTGGTCTTGAAGGG
CAGCGATGAACCCTACCGGATGATGACCAGCCGCGTGGAACATCGCCTGATTGTCCGCCAGGACAACGCCGATGAGCGGC
TCACCGAGTTGGGCGTGAAGTTGGGACTGGTGGACGAGGACACGCAGCGGGCGGTCCAGGCCAAGTACCGCCGGGTGGCT
GAGGGCATACGGGCGCTTCAAATGCAGCGCGTCCAGGGCCAGACCGGTGACGCCTGGCTGCGCCGTCCCGAGTTTGCCCT
GGAGGATGTAGAGGCGCTTGGCTTCAAGCTCCCCGCCCTTTCTCCAGAGGAGCGAGAAGCTGTGGCGATCCGAGTGAAGT
ACGCCGGGTATATCGAGCGGGCTGAACGTCAACTGGCGGCCGAGGACCGGGCCAGGGAACTCAGTCTGCGGGGCGTGGCG
TTTGGCGAGATCGCGTCGCTCTCCAATGAGGCTCGGGAGAAGCTGGAGCGGGTCCGTCCCTTAACGGTGGCGCAGGCGGC
GGGAATTCCGGGCGTCCGCCACGCCGACATCAGCGCCCTCTTGGTGCATCTGCGGCGCACAGGCAACGTTTCACGCGAAA
CCTGA

Upstream 100 bases:

>100_bases
TGCCTTTTCCCTAGCCAAGCTTGGTCTAGCTAACGCTCGTTAGGCATTCTAGCCTTTCACCCGCGGCAGGGCGGCCACCC
CTACCGTGACCGCCGACGGA

Downstream 100 bases:

>100_bases
CGGTGAAACAAGGAAGGCGGGGGATTTGGCCAAGCTCTTTTCGCTTGACGCCCCCCCTGCTTCACGGGAAACTTGACGGT
GAAACAGGGGGCGGGACTTG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MLAASPLLHFGDMRGWNVMVIGGGHAGLEAAWAAAKFARVAVLVSNPATVGRMPCNPAVGGPGKSQLVFEVQALGGLMGR
LADDTAIHTRVLNASKGPAVQSLRVQNERDAYAERAQEILLGHSGIDVVRGEAADLEPDGCGGWFVVTTDGRRFHARSVV
VAAGTFMRGVTWYGRYSRPEGRQGEPPSRFLSAPLARAGHRLKRYKTGTPPRVRADSVRFADLLEIPADPQPRGFTGRPG
PRAAESPTWQTHTTPETHRLIQENLHESPMYAGDIAGLGPRYCPSIEDKVVRFAHHDRHLLFVEPDGVQTSEVYLQGFSS
SLPPALQDRLVRSLPGFEAAVIQRYAYAVEYDVVDSTELTLNLESRLLPGIFTAGQINGTSGYEEAAAQGLVAGTAAARR
ALELEELQISRETSYLGVLLDDLVLKGSDEPYRMMTSRVEHRLIVRQDNADERLTELGVKLGLVDEDTQRAVQAKYRRVA
EGIRALQMQRVQGQTGDAWLRRPEFALEDVEALGFKLPALSPEEREAVAIRVKYAGYIERAERQLAAEDRARELSLRGVA
FGEIASLSNEAREKLERVRPLTVAQAAGIPGVRHADISALLVHLRRTGNVSRET

Sequences:

>Translated_614_residues
MLAASPLLHFGDMRGWNVMVIGGGHAGLEAAWAAAKFARVAVLVSNPATVGRMPCNPAVGGPGKSQLVFEVQALGGLMGR
LADDTAIHTRVLNASKGPAVQSLRVQNERDAYAERAQEILLGHSGIDVVRGEAADLEPDGCGGWFVVTTDGRRFHARSVV
VAAGTFMRGVTWYGRYSRPEGRQGEPPSRFLSAPLARAGHRLKRYKTGTPPRVRADSVRFADLLEIPADPQPRGFTGRPG
PRAAESPTWQTHTTPETHRLIQENLHESPMYAGDIAGLGPRYCPSIEDKVVRFAHHDRHLLFVEPDGVQTSEVYLQGFSS
SLPPALQDRLVRSLPGFEAAVIQRYAYAVEYDVVDSTELTLNLESRLLPGIFTAGQINGTSGYEEAAAQGLVAGTAAARR
ALELEELQISRETSYLGVLLDDLVLKGSDEPYRMMTSRVEHRLIVRQDNADERLTELGVKLGLVDEDTQRAVQAKYRRVA
EGIRALQMQRVQGQTGDAWLRRPEFALEDVEALGFKLPALSPEEREAVAIRVKYAGYIERAERQLAAEDRARELSLRGVA
FGEIASLSNEAREKLERVRPLTVAQAAGIPGVRHADISALLVHLRRTGNVSRET
>Mature_614_residues
MLAASPLLHFGDMRGWNVMVIGGGHAGLEAAWAAAKFARVAVLVSNPATVGRMPCNPAVGGPGKSQLVFEVQALGGLMGR
LADDTAIHTRVLNASKGPAVQSLRVQNERDAYAERAQEILLGHSGIDVVRGEAADLEPDGCGGWFVVTTDGRRFHARSVV
VAAGTFMRGVTWYGRYSRPEGRQGEPPSRFLSAPLARAGHRLKRYKTGTPPRVRADSVRFADLLEIPADPQPRGFTGRPG
PRAAESPTWQTHTTPETHRLIQENLHESPMYAGDIAGLGPRYCPSIEDKVVRFAHHDRHLLFVEPDGVQTSEVYLQGFSS
SLPPALQDRLVRSLPGFEAAVIQRYAYAVEYDVVDSTELTLNLESRLLPGIFTAGQINGTSGYEEAAAQGLVAGTAAARR
ALELEELQISRETSYLGVLLDDLVLKGSDEPYRMMTSRVEHRLIVRQDNADERLTELGVKLGLVDEDTQRAVQAKYRRVA
EGIRALQMQRVQGQTGDAWLRRPEFALEDVEALGFKLPALSPEEREAVAIRVKYAGYIERAERQLAAEDRARELSLRGVA
FGEIASLSNEAREKLERVRPLTVAQAAGIPGVRHADISALLVHLRRTGNVSRET

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=636, Percent_Identity=38.9937106918239, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI19882217, Length=661, Percent_Identity=37.9727685325265, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI183227703, Length=677, Percent_Identity=36.7799113737075, Blast_Score=325, Evalue=7e-89,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=43.1781701444623, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17534255, Length=617, Percent_Identity=37.1150729335494, Blast_Score=355, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6321202, Length=651, Percent_Identity=36.405529953917, Blast_Score=351, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=38.1933438985737, Blast_Score=351, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 67166; Mature: 67166

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAASPLLHFGDMRGWNVMVIGGGHAGLEAAWAAAKFARVAVLVSNPATVGRMPCNPAVG
CCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
GPGKSQLVFEVQALGGLMGRLADDTAIHTRVLNASKGPAVQSLRVQNERDAYAERAQEIL
CCCCCEEEEEHHHHHHHHHHHCCCHHHHHHEECCCCCCCHHHHCCCCHHHHHHHHHHHHH
LGHSGIDVVRGEAADLEPDGCGGWFVVTTDGRRFHARSVVVAAGTFMRGVTWYGRYSRPE
HCCCCCCEECCCCCCCCCCCCCCEEEEECCCCEEECCEEEEEHHHHHHCCCCCCCCCCCC
GRQGEPPSRFLSAPLARAGHRLKRYKTGTPPRVRADSVRFADLLEIPADPQPRGFTGRPG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
PRAAESPTWQTHTTPETHRLIQENLHESPMYAGDIAGLGPRYCPSIEDKVVRFAHHDRHL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEE
LFVEPDGVQTSEVYLQGFSSSLPPALQDRLVRSLPGFEAAVIQRYAYAVEYDVVDSTELT
EEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCEEE
LNLESRLLPGIFTAGQINGTSGYEEAAAQGLVAGTAAARRALELEELQISRETSYLGVLL
EEEHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
DDLVLKGSDEPYRMMTSRVEHRLIVRQDNADERLTELGVKLGLVDEDTQRAVQAKYRRVA
HHHHHCCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEECCCCHHHHHHHHHHHHHH
EGIRALQMQRVQGQTGDAWLRRPEFALEDVEALGFKLPALSPEEREAVAIRVKYAGYIER
HHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHCEECCCCCCCCCCEEEEEEEHHHHHHH
AERQLAAEDRARELSLRGVAFGEIASLSNEAREKLERVRPLTVAQAAGIPGVRHADISAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHH
LVHLRRTGNVSRET
HHHHHHCCCCCCCC
>Mature Secondary Structure
MLAASPLLHFGDMRGWNVMVIGGGHAGLEAAWAAAKFARVAVLVSNPATVGRMPCNPAVG
CCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
GPGKSQLVFEVQALGGLMGRLADDTAIHTRVLNASKGPAVQSLRVQNERDAYAERAQEIL
CCCCCEEEEEHHHHHHHHHHHCCCHHHHHHEECCCCCCCHHHHCCCCHHHHHHHHHHHHH
LGHSGIDVVRGEAADLEPDGCGGWFVVTTDGRRFHARSVVVAAGTFMRGVTWYGRYSRPE
HCCCCCCEECCCCCCCCCCCCCCEEEEECCCCEEECCEEEEEHHHHHHCCCCCCCCCCCC
GRQGEPPSRFLSAPLARAGHRLKRYKTGTPPRVRADSVRFADLLEIPADPQPRGFTGRPG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
PRAAESPTWQTHTTPETHRLIQENLHESPMYAGDIAGLGPRYCPSIEDKVVRFAHHDRHL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEE
LFVEPDGVQTSEVYLQGFSSSLPPALQDRLVRSLPGFEAAVIQRYAYAVEYDVVDSTELT
EEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCEEE
LNLESRLLPGIFTAGQINGTSGYEEAAAQGLVAGTAAARRALELEELQISRETSYLGVLL
EEEHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
DDLVLKGSDEPYRMMTSRVEHRLIVRQDNADERLTELGVKLGLVDEDTQRAVQAKYRRVA
HHHHHCCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEECCCCHHHHHHHHHHHHHH
EGIRALQMQRVQGQTGDAWLRRPEFALEDVEALGFKLPALSPEEREAVAIRVKYAGYIER
HHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHCEECCCCCCCCCCEEEEEEEHHHHHHH
AERQLAAEDRARELSLRGVAFGEIASLSNEAREKLERVRPLTVAQAAGIPGVRHADISAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHH
LVHLRRTGNVSRET
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA