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The map label for this gene is trpF [H]

Identifier: 94986365

GI number: 94986365

Start: 2391762

End: 2392406

Strand: Reverse

Name: trpF [H]

Synonym: Dgeo_2266

Alternate gene names: 94986365

Gene position: 2392406-2391762 (Counterclockwise)

Preceding gene: 94986366

Following gene: 94986363

Centisome position: 96.97

GC content: 68.53

Gene sequence:

>645_bases
GTGACCGCCGGTCTCCCCGTTCGCGTCAAGATCTGCGGTACCACCAGCCTGCATGACGCTCTGCTGGCCGCCCAGGCAGG
GGCTGACGCGCTGGGCTTTATCTTTGCGCCAGGCAGCCGGCGGCGGGTGTCCCCGCAGATTGCGCGCGAGGCCGGCTTGA
GCGTGGGGCCGGTGATCGCGCGGGTGGGCGTGTTTCTGAACCAGCCGCTGGACGAGGTGTTGCGAACGGCCGAAGCGGCG
CGGGTGAGCGCCATACAGCTTCACGGTGTCCTGTCAAGTCTTTACGTCCGTGAGGTGGCCCGCTATTATCCCGTTCTGCG
CGTTCTGCGCCCCGCCGATTTGGGACGGGAGGACGTGCTGACAGCCCTGAGCTGTCCGGGCGTCACGCCGATGCTGGACG
CGCCGGAGCCGGGCAGTGGGGTACCGCTGGACTGGGCCGCGTTGCGGGAGAGCTTCCCGGTGGGAGCCTGGCTGGCCGGT
GGGTTGGGCGCCGCCAATGTGGCGGAGGCGATCCGGGTGCTGCGGCCCGCAGGTGTGGACGCTGTGAGTCGCCTGGAGAA
GGCCCCGGGCGTGAAAGACGCGGAGCAGGTGAAGGCCTTTGTCCGGGCTGCACGAGGAGCCAAAACACAGAGTTATCCAC
AGTGA

Upstream 100 bases:

>100_bases
AACGGCCCAAGCTGTATGACCACTACGCGCTGCTCCTGACAGCCCTGCTGCGCCGAGTGCGCGACCTGGAAGCGCGTGTG
GCGGCGCTGGAGGCTCGCGA

Downstream 100 bases:

>100_bases
AACGCGTGCCTGTGGATAACTCTGTGGCGGCGCCTCCCTCGAGTCTGCAAGAAGAAAAATGCTGCTATAGACAGCAAAAA
TCATATGGCTTATGGAGGGC

Product: phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: PRAI [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTAGLPVRVKICGTTSLHDALLAAQAGADALGFIFAPGSRRRVSPQIAREAGLSVGPVIARVGVFLNQPLDEVLRTAEAA
RVSAIQLHGVLSSLYVREVARYYPVLRVLRPADLGREDVLTALSCPGVTPMLDAPEPGSGVPLDWAALRESFPVGAWLAG
GLGAANVAEAIRVLRPAGVDAVSRLEKAPGVKDAEQVKAFVRAARGAKTQSYPQ

Sequences:

>Translated_214_residues
MTAGLPVRVKICGTTSLHDALLAAQAGADALGFIFAPGSRRRVSPQIAREAGLSVGPVIARVGVFLNQPLDEVLRTAEAA
RVSAIQLHGVLSSLYVREVARYYPVLRVLRPADLGREDVLTALSCPGVTPMLDAPEPGSGVPLDWAALRESFPVGAWLAG
GLGAANVAEAIRVLRPAGVDAVSRLEKAPGVKDAEQVKAFVRAARGAKTQSYPQ
>Mature_213_residues
TAGLPVRVKICGTTSLHDALLAAQAGADALGFIFAPGSRRRVSPQIAREAGLSVGPVIARVGVFLNQPLDEVLRTAEAAR
VSAIQLHGVLSSLYVREVARYYPVLRVLRPADLGREDVLTALSCPGVTPMLDAPEPGSGVPLDWAALRESFPVGAWLAGG
LGAANVAEAIRVLRPAGVDAVSRLEKAPGVKDAEQVKAFVRAARGAKTQSYPQ

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=206, Percent_Identity=33.9805825242718, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320210, Length=213, Percent_Identity=32.8638497652582, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00697 PRAI [H]

EC number: =5.3.1.24 [H]

Molecular weight: Translated: 22391; Mature: 22260

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGLPVRVKICGTTSLHDALLAAQAGADALGFIFAPGSRRRVSPQIAREAGLSVGPVIA
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHH
RVGVFLNQPLDEVLRTAEAARVSAIQLHGVLSSLYVREVARYYPVLRVLRPADLGREDVL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TALSCPGVTPMLDAPEPGSGVPLDWAALRESFPVGAWLAGGLGAANVAEAIRVLRPAGVD
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHCCCCHH
AVSRLEKAPGVKDAEQVKAFVRAARGAKTQSYPQ
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAGLPVRVKICGTTSLHDALLAAQAGADALGFIFAPGSRRRVSPQIAREAGLSVGPVIA
CCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHH
RVGVFLNQPLDEVLRTAEAARVSAIQLHGVLSSLYVREVARYYPVLRVLRPADLGREDVL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TALSCPGVTPMLDAPEPGSGVPLDWAALRESFPVGAWLAGGLGAANVAEAIRVLRPAGVD
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHCCCCHH
AVSRLEKAPGVKDAEQVKAFVRAARGAKTQSYPQ
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]