Click here to switch to the map view.

The map label for this gene is hisA

Identifier: 94986257

GI number: 94986257

Start: 2274277

End: 2275008

Strand: Reverse

Name: hisA

Synonym: Dgeo_2158

Alternate gene names: 94986257

Gene position: 2275008-2274277 (Counterclockwise)

Preceding gene: 94986258

Following gene: 94986255

Centisome position: 92.21

GC content: 68.31

Gene sequence:

>732_bases
ATGACGCCCGTCTCTTCCCGCCTTCAGCCTCTGATCATTCCCTGCGTGGACATCCAGTCGGGCCGCGCGGTGCGCCTCTA
TGAGGGTGACCCCGACCGCGAGACGGTCTACTTCGAGTCGCCTCTGGAGGCGGCCCGCCACTGGGCTGCCTTGGGTGCGG
GGCTGGTGCATCTGGTGGACCTGGACGCCGCCACCGGACGCGGTGAGAACCGCGCAGTCATCACGCAGATCACCGCCGAA
CTGGGCGTACCGGTCGAGGTGGGGGGCGGCATTCGCGACCGCGCCGCTGCCGAAGCCCTGCTGGACGCCGGAGTGGGACG
GGTGGTGATCGGCACCGCCGCTGTGAAGAACCCGGCACTCGTGCGCGAGCTGATTGCCGCGCACGGCCCCAGTCGGGTTG
TCGTGAGCCTGGACGCACGCGGCCTGGACGTCGCTATCCACGGGTGGGCCGAAGGCAGCGGCGTCAGCGTTGCGGAACTC
GCGCCGCTGTTGGCAGAGGCGGGCCTTGAAACGCTGATCTTTACAGACGTGACCCGTGACGGCACCCTCCGGGGTCTGGA
CCGTGACCTGATGCGGCAGGTCCGCCAGCTCTGGTCGAACACCCTGATTGTCGGCGGCGGCGTTGCCACGCTGGACGATG
TGCGCTTGTTGGCCGAGGAAGGTATTCAGGGCGCCATCGTGGGCCGGGCGATCTACGAGGGGACGCTGGTCTATCCAGTG
ACGGGTCTCTGA

Upstream 100 bases:

>100_bases
AAACGGATATCGCCTTCTTTCTGGAAGAAGGGTGATCCCGTAAGCGGCGCGGCTGCGGCCCCCATGATCTGCGTTTCTCG
GGCGCCGCTATCCTGCCCCC

Downstream 100 bases:

>100_bases
ATACACACCACTACGCCTGCTCTGGCCCTCAGCGTTTTTTTTGACGGCCTGTCAGGCCCAGCAGCCGCAGCACGATCACG
GCGGGGACGGCGCAGAGGGC

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTPVSSRLQPLIIPCVDIQSGRAVRLYEGDPDRETVYFESPLEAARHWAALGAGLVHLVDLDAATGRGENRAVITQITAE
LGVPVEVGGGIRDRAAAEALLDAGVGRVVIGTAAVKNPALVRELIAAHGPSRVVVSLDARGLDVAIHGWAEGSGVSVAEL
APLLAEAGLETLIFTDVTRDGTLRGLDRDLMRQVRQLWSNTLIVGGGVATLDDVRLLAEEGIQGAIVGRAIYEGTLVYPV
TGL

Sequences:

>Translated_243_residues
MTPVSSRLQPLIIPCVDIQSGRAVRLYEGDPDRETVYFESPLEAARHWAALGAGLVHLVDLDAATGRGENRAVITQITAE
LGVPVEVGGGIRDRAAAEALLDAGVGRVVIGTAAVKNPALVRELIAAHGPSRVVVSLDARGLDVAIHGWAEGSGVSVAEL
APLLAEAGLETLIFTDVTRDGTLRGLDRDLMRQVRQLWSNTLIVGGGVATLDDVRLLAEEGIQGAIVGRAIYEGTLVYPV
TGL
>Mature_242_residues
TPVSSRLQPLIIPCVDIQSGRAVRLYEGDPDRETVYFESPLEAARHWAALGAGLVHLVDLDAATGRGENRAVITQITAEL
GVPVEVGGGIRDRAAAEALLDAGVGRVVIGTAAVKNPALVRELIAAHGPSRVVVSLDARGLDVAIHGWAEGSGVSVAELA
PLLAEAGLETLIFTDVTRDGTLRGLDRDLMRQVRQLWSNTLIVGGGVATLDDVRLLAEEGIQGAIVGRAIYEGTLVYPVT
GL

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=233, Percent_Identity=38.1974248927039, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_DEIGD (Q1IWD2)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605621.1
- ProteinModelPortal:   Q1IWD2
- STRING:   Q1IWD2
- GeneID:   4058893
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_2158
- NMPDR:   fig|68909.1.peg.1988
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   Q1IWD2
- ProtClustDB:   CLSK497064
- BioCyc:   DGEO319795:DGEO_2158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25476; Mature: 25344

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: ACT_SITE 17-17 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVSSRLQPLIIPCVDIQSGRAVRLYEGDPDRETVYFESPLEAARHWAALGAGLVHLVD
CCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHHCCEEEEEE
LDAATGRGENRAVITQITAELGVPVEVGGGIRDRAAAEALLDAGVGRVVIGTAAVKNPAL
CCCCCCCCCCCEEEEEEHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHCCHHH
VRELIAAHGPSRVVVSLDARGLDVAIHGWAEGSGVSVAELAPLLAEAGLETLIFTDVTRD
HHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
GTLRGLDRDLMRQVRQLWSNTLIVGGGVATLDDVRLLAEEGIQGAIVGRAIYEGTLVYPV
CCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHCCEEEEEE
TGL
CCC
>Mature Secondary Structure 
TPVSSRLQPLIIPCVDIQSGRAVRLYEGDPDRETVYFESPLEAARHWAALGAGLVHLVD
CCCCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHHCCEEEEEE
LDAATGRGENRAVITQITAELGVPVEVGGGIRDRAAAEALLDAGVGRVVIGTAAVKNPAL
CCCCCCCCCCCEEEEEEHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHCCHHH
VRELIAAHGPSRVVVSLDARGLDVAIHGWAEGSGVSVAELAPLLAEAGLETLIFTDVTRD
HHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
GTLRGLDRDLMRQVRQLWSNTLIVGGGVATLDDVRLLAEEGIQGAIVGRAIYEGTLVYPV
CCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHCCEEEEEE
TGL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA