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The map label for this gene is birA [H]

Identifier: 94986239

GI number: 94986239

Start: 2253836

End: 2254810

Strand: Reverse

Name: birA [H]

Synonym: Dgeo_2140

Alternate gene names: 94986239

Gene position: 2254810-2253836 (Counterclockwise)

Preceding gene: 94986246

Following gene: 94986238

Centisome position: 91.39

GC content: 71.28

Gene sequence:

>975_bases
TTGCCTTCGTTCAGCAAATCCTCTACAACACTGAACGTGCCCGCCCGCCTGCTGCCGCTGCTCACCGAGGTGCCCCAGTC
CGGGGAGATTCTCGGTGCCCGGCTGGGTGTGGGCCGCGTGACCGTCAACACTCTCGCCCGGCGCCTGGCCGAAGATGGAG
TGCCGGTTGTGGTCACGCGGGGCGGGTACGCGCTCGCGCCGGGGACCCCTGCACCGCAGCTGGTGCCGGTCAGGGGCACC
TTTGGGCAGGCCATGCGCTATTGCGGCACGGTGGGCAGCACCCAAGACGAGGCCCGCGTCTGGGCCGACGATCCGCAGGC
TCCGGCTCCTCACGGCGCGGTGGTGGTTGCGGAACGGCAGACCGCCGGACGGGGGCGGCGCGGACGCAGCTGGGACACCA
CGCACGGCACGCTGGTCTTGAGTGTGCTGCTGCACGGACCGTTGCCCCTGCCTGAGCTGAGTCTGATGCCGCTGGCCGCA
GGGGTCGCCCTTCAAGCCGCTTGCGGGGTCGGTGGGCTGAAGTGGCCCAACGACCTGCTTGCCCCGGACGGTCGCAAGCT
CGCGGGCATCCTCCTCGAAGCGGACCTGCGCGGCGAGGAGGCGCGGCGAGCGGTCCTCGGTATCGGCGTGAATGTAGGTG
CTGCGCCGGAGGGGGCCGCCTGCCTCTGCGAGTTCAGACCTGAGCTCACCCGTGCGGGCCTGCTTGCCTGCATTCTGGCT
GAACTGGAACGCTGGCTGGCCGCGCCACCCGCAGCCGTGCTGGAGGCGTGGCGGGCGGCCAACGTGACGCTCGGTCGGGA
CGTTCGCGTGTCCACGCCGCGCGGCCTCCTGTCGGGCACTGCCCTCGACCTCGACCCCCAGGGGGGTCTCCTCATCCGCA
CGCCGGAAGGCACCACCGTCACCGCCCACGCGGGCGACGTTCAGCTGGTCGGTTCCCTCGCCCCCTCACACGGTCCACAG
GAGGACACCCCATGA

Upstream 100 bases:

>100_bases
ATAGCAAAAGGTCTGCTGCAGAGCGGACCCTGGCCTCACGGTTGGGCTGGCGGAGGGAAGACCAGCTTGCCCCGTCCATC
CCTCCTTTGGACTCAGTTCA

Downstream 100 bases:

>100_bases
CCCAAGTCACGCATCCGACCCTCGCCCGCGTGCTCGCGCCCGCACCCAGCCTCAGCCGTGACCTAGGGCTGGTTCTCGGT
GGCGCGGCTCTGGTCGCGCT

Product: bifunctional BirA, biotin operon repressor/biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MPSFSKSSTTLNVPARLLPLLTEVPQSGEILGARLGVGRVTVNTLARRLAEDGVPVVVTRGGYALAPGTPAPQLVPVRGT
FGQAMRYCGTVGSTQDEARVWADDPQAPAPHGAVVVAERQTAGRGRRGRSWDTTHGTLVLSVLLHGPLPLPELSLMPLAA
GVALQAACGVGGLKWPNDLLAPDGRKLAGILLEADLRGEEARRAVLGIGVNVGAAPEGAACLCEFRPELTRAGLLACILA
ELERWLAAPPAAVLEAWRAANVTLGRDVRVSTPRGLLSGTALDLDPQGGLLIRTPEGTTVTAHAGDVQLVGSLAPSHGPQ
EDTP

Sequences:

>Translated_324_residues
MPSFSKSSTTLNVPARLLPLLTEVPQSGEILGARLGVGRVTVNTLARRLAEDGVPVVVTRGGYALAPGTPAPQLVPVRGT
FGQAMRYCGTVGSTQDEARVWADDPQAPAPHGAVVVAERQTAGRGRRGRSWDTTHGTLVLSVLLHGPLPLPELSLMPLAA
GVALQAACGVGGLKWPNDLLAPDGRKLAGILLEADLRGEEARRAVLGIGVNVGAAPEGAACLCEFRPELTRAGLLACILA
ELERWLAAPPAAVLEAWRAANVTLGRDVRVSTPRGLLSGTALDLDPQGGLLIRTPEGTTVTAHAGDVQLVGSLAPSHGPQ
EDTP
>Mature_323_residues
PSFSKSSTTLNVPARLLPLLTEVPQSGEILGARLGVGRVTVNTLARRLAEDGVPVVVTRGGYALAPGTPAPQLVPVRGTF
GQAMRYCGTVGSTQDEARVWADDPQAPAPHGAVVVAERQTAGRGRRGRSWDTTHGTLVLSVLLHGPLPLPELSLMPLAAG
VALQAACGVGGLKWPNDLLAPDGRKLAGILLEADLRGEEARRAVLGIGVNVGAAPEGAACLCEFRPELTRAGLLACILAE
LERWLAAPPAAVLEAWRAANVTLGRDVRVSTPRGLLSGTALDLDPQGGLLIRTPEGTTVTAHAGDVQLVGSLAPSHGPQE
DTP

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=300, Percent_Identity=32.3333333333333, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 33504; Mature: 33373

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFSKSSTTLNVPARLLPLLTEVPQSGEILGARLGVGRVTVNTLARRLAEDGVPVVVTR
CCCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCCEEEEC
GGYALAPGTPAPQLVPVRGTFGQAMRYCGTVGSTQDEARVWADDPQAPAPHGAVVVAERQ
CCEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEEEECC
TAGRGRRGRSWDTTHGTLVLSVLLHGPLPLPELSLMPLAAGVALQAACGVGGLKWPNDLL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
APDGRKLAGILLEADLRGEEARRAVLGIGVNVGAAPEGAACLCEFRPELTRAGLLACILA
CCCCCEEEEEEEEECCCCHHHCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
ELERWLAAPPAAVLEAWRAANVTLGRDVRVSTPRGLLSGTALDLDPQGGLLIRTPEGTTV
HHHHHHCCCCHHHHHHHHHCCEEECCCEEECCCCCHHCCCEEEECCCCCEEEECCCCCEE
TAHAGDVQLVGSLAPSHGPQEDTP
EEECCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PSFSKSSTTLNVPARLLPLLTEVPQSGEILGARLGVGRVTVNTLARRLAEDGVPVVVTR
CCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCCEEEEC
GGYALAPGTPAPQLVPVRGTFGQAMRYCGTVGSTQDEARVWADDPQAPAPHGAVVVAERQ
CCEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEEEECC
TAGRGRRGRSWDTTHGTLVLSVLLHGPLPLPELSLMPLAAGVALQAACGVGGLKWPNDLL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
APDGRKLAGILLEADLRGEEARRAVLGIGVNVGAAPEGAACLCEFRPELTRAGLLACILA
CCCCCEEEEEEEEECCCCHHHCEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
ELERWLAAPPAAVLEAWRAANVTLGRDVRVSTPRGLLSGTALDLDPQGGLLIRTPEGTTV
HHHHHHCCCCHHHHHHHHHCCEEECCCEEECCCCCHHCCCEEEECCCCCEEEECCCCCEE
TAHAGDVQLVGSLAPSHGPQEDTP
EEECCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]