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The map label for this gene is ddl

Identifier: 94986220

GI number: 94986220

Start: 2232948

End: 2233967

Strand: Reverse

Name: ddl

Synonym: Dgeo_2121

Alternate gene names: 94986220

Gene position: 2233967-2232948 (Counterclockwise)

Preceding gene: 94986224

Following gene: 94986219

Centisome position: 90.55

GC content: 67.35

Gene sequence:

>1020_bases
GTGAAGAAGCGCATCCTGCTGCTGGCGGGCGGACAATCTGAAGAGCACGAAGTGAGCCTGAGGAGTGCCCGCAGCGTCCT
GGCGGCTTTGCCCCGCGATCAGTTCGACGTGACGCCCGTGGTGATCAGCCCTCAGGGGCGCTGGTTGCCCCCCACTGACA
CCCAGCGGGCCCTGGAAACCGGTGAAGCCGTGCGCGGCGGCGACCTGGTGCTGCACCGCGCCGCCAGCGCCGAGGGCTAC
GACGCCGTTTTCCCTCTGCTGCATGGCCCAATGGGCGAGGACGGCACCATCCAGGGCTTGCTGACGCTGGCGGGAATTCC
CTTCGTGGGGTCGGGGGTGCTGGGATCAGCGGTCAGCATGGACAAGGTGATGACCAAGCAGGTGCTGGCCTCGGTGGGCA
TGCCGCAGGTGGCCTGGCGCCTGGCCGTGCGCCGCGAGTGGCAGGAACGCCCGCAGGAGGTGCGCGCCCGCGCCGGGGAG
CTGGGCTACCCCCTCTTCGTGAAGCCCGCCAATCTGGGATCGAGCGTCGGCATCAGCAAGGTGAGTGGCCCCGGCGAGCT
GGAGCGGGCCCTCGACCTTGCCTTTAGCCTCGGCCGCCGCGTGATCTTGGAGGCGATGACCGCCCACAAGCCGCGCGAGT
TGGAGGTGGGCATCCTGGGCAACGACGCGCCTATCGCCAGCCCGGTCGGGGAGCTGCGTTTCGAGGCCGACTTTTACGAC
TACGAGACGAAATACACCGAGGGCCGCGCGACCATGCACATTCCCGCCCCGCTGCCTCCCGAAATCGCCGAGCGTGTGCG
GATGCTGGCCCTCACGGCCTTCCGCACGCTCGACTGTGCCGGCTTGGCCCGGGTGGACTTTTTCTACGTGGAGCAGACCG
GTGAACTCTTCCTCAACGAGGTGAACACCATGCCTGGCTTCACCACAACCAGCATGTACCCCAAGCTTTTCGAGGCGGCG
GGCCTGAGTTACAGCGAACTGGTCACGCGCCTGGTGGAGTTGGCGCTGGAACGGCGGTAA

Upstream 100 bases:

>100_bases
CGGCAGATGTGAGAAGTGGGCAAGAAGATCGGGGGAAGACATCATCCACCGGTCAGGTCCCTCCAGCTGTCGGTCGCGCG
GCTGAGGCATACTGCCCCGC

Downstream 100 bases:

>100_bases
GTGGGTCGTCGCAGCGGTAGCTTCAACGGTGGCTTAACCTGAACACGCCCTGGCGTCACGCCTTAACGGGACAGGCGTCC
TTGGCACCCCCCCGGATAGG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKKRILLLAGGQSEEHEVSLRSARSVLAALPRDQFDVTPVVISPQGRWLPPTDTQRALETGEAVRGGDLVLHRAASAEGY
DAVFPLLHGPMGEDGTIQGLLTLAGIPFVGSGVLGSAVSMDKVMTKQVLASVGMPQVAWRLAVRREWQERPQEVRARAGE
LGYPLFVKPANLGSSVGISKVSGPGELERALDLAFSLGRRVILEAMTAHKPRELEVGILGNDAPIASPVGELRFEADFYD
YETKYTEGRATMHIPAPLPPEIAERVRMLALTAFRTLDCAGLARVDFFYVEQTGELFLNEVNTMPGFTTTSMYPKLFEAA
GLSYSELVTRLVELALERR

Sequences:

>Translated_339_residues
MKKRILLLAGGQSEEHEVSLRSARSVLAALPRDQFDVTPVVISPQGRWLPPTDTQRALETGEAVRGGDLVLHRAASAEGY
DAVFPLLHGPMGEDGTIQGLLTLAGIPFVGSGVLGSAVSMDKVMTKQVLASVGMPQVAWRLAVRREWQERPQEVRARAGE
LGYPLFVKPANLGSSVGISKVSGPGELERALDLAFSLGRRVILEAMTAHKPRELEVGILGNDAPIASPVGELRFEADFYD
YETKYTEGRATMHIPAPLPPEIAERVRMLALTAFRTLDCAGLARVDFFYVEQTGELFLNEVNTMPGFTTTSMYPKLFEAA
GLSYSELVTRLVELALERR
>Mature_339_residues
MKKRILLLAGGQSEEHEVSLRSARSVLAALPRDQFDVTPVVISPQGRWLPPTDTQRALETGEAVRGGDLVLHRAASAEGY
DAVFPLLHGPMGEDGTIQGLLTLAGIPFVGSGVLGSAVSMDKVMTKQVLASVGMPQVAWRLAVRREWQERPQEVRARAGE
LGYPLFVKPANLGSSVGISKVSGPGELERALDLAFSLGRRVILEAMTAHKPRELEVGILGNDAPIASPVGELRFEADFYD
YETKYTEGRATMHIPAPLPPEIAERVRMLALTAFRTLDCAGLARVDFFYVEQTGELFLNEVNTMPGFTTTSMYPKLFEAA
GLSYSELVTRLVELALERR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=43.1754874651811, Blast_Score=280, Evalue=7e-77,
Organism=Escherichia coli, GI1786280, Length=336, Percent_Identity=36.3095238095238, Blast_Score=181, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_DEIGD (Q1IWG9)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605584.1
- ProteinModelPortal:   Q1IWG9
- SMR:   Q1IWG9
- STRING:   Q1IWG9
- GeneID:   4058856
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_2121
- NMPDR:   fig|68909.1.peg.1950
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   CKAINAY
- PhylomeDB:   Q1IWG9
- ProtClustDB:   PRK01966
- BioCyc:   DGEO319795:DGEO_2121-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 36973; Mature: 36973

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRILLLAGGQSEEHEVSLRSARSVLAALPRDQFDVTPVVISPQGRWLPPTDTQRALET
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
GEAVRGGDLVLHRAASAEGYDAVFPLLHGPMGEDGTIQGLLTLAGIPFVGSGVLGSAVSM
CCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
DKVMTKQVLASVGMPQVAWRLAVRREWQERPQEVRARAGELGYPLFVKPANLGSSVGISK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
VSGPGELERALDLAFSLGRRVILEAMTAHKPRELEVGILGNDAPIASPVGELRFEADFYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHEEECCEEC
YETKYTEGRATMHIPAPLPPEIAERVRMLALTAFRTLDCAGLARVDFFYVEQTGELFLNE
CCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECHHHHHHHH
VNTMPGFTTTSMYPKLFEAAGLSYSELVTRLVELALERR
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKRILLLAGGQSEEHEVSLRSARSVLAALPRDQFDVTPVVISPQGRWLPPTDTQRALET
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
GEAVRGGDLVLHRAASAEGYDAVFPLLHGPMGEDGTIQGLLTLAGIPFVGSGVLGSAVSM
CCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
DKVMTKQVLASVGMPQVAWRLAVRREWQERPQEVRARAGELGYPLFVKPANLGSSVGISK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
VSGPGELERALDLAFSLGRRVILEAMTAHKPRELEVGILGNDAPIASPVGELRFEADFYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHEEECCEEC
YETKYTEGRATMHIPAPLPPEIAERVRMLALTAFRTLDCAGLARVDFFYVEQTGELFLNE
CCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECHHHHHHHH
VNTMPGFTTTSMYPKLFEAAGLSYSELVTRLVELALERR
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA