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The map label for this gene is trmB

Identifier: 94986189

GI number: 94986189

Start: 2199696

End: 2200691

Strand: Reverse

Name: trmB

Synonym: Dgeo_2090

Alternate gene names: 94986189

Gene position: 2200691-2199696 (Counterclockwise)

Preceding gene: 94986190

Following gene: 94986188

Centisome position: 89.2

GC content: 66.47

Gene sequence:

>996_bases
ATGATCTTCCGGCTCGCAGACTTCCACTTTCCCGATAGCGCCGCCCGTCTGTATCCCGACACACCGCAACGCCCCTGGAT
CCTGGAGGTTGGCTTTGGCGACGGGCGCTTCTGGCCGCACTACGCGGCGACCTTTCCCGAGGCGCCGAATTATCTCGGCG
TTGAGATCAGCGGCGTCTCGCTCCTCAAGGCCGAACGGCGGCTCCGGGAGGCGGGGCTGTCAAACGCCGTGCTGACTAAA
CTCCCCGCCACCCCCCTGATCCGCGAGGTGGTGCCCGCAGGGTCCCTCGACGCCATCGTGGTGAATTTTCCTGACCCTTG
GCCCAAGGCTGGACACGCAGAACACCGTCTGTTGCGCGCCCCGTTCTTTCGCCTCGCTGCCAGCCGCTTGAAACCGGGCG
GGGCAGTCCTTTTGACCACCGACCACGACGAGTATTTCGAGTTCGCCTGCCGCGAGGCGGAGGCCAGCGGCGTGATGCGG
GCAGAACTCACCGATCCTCCCCCTGCCGCCCTCGAAACCAAGTACGCGCGCAAGTGGCGCGAGCTGGGGCTGGAGGTCCA
GCATGCTCGCTTTGTTCCAACCCATCACCCCCATGTGCCACACGGCACCGTGGCCCGTTTCCCTGACTCGGAGGATGCTC
CCGCCGTGCCCCACGCCATCCTGACCCTGCCCGCTGCCTTTGATCCCGGCGCCTTCCACAAGCACACGGCCCGCGGTGGC
CAGACCCGTGAAGACCCAGTGGGCTGGACGGTCGTCCTGCTCGAGCTCTACCGCAGTCTTAAGCAGGACGGCTGGGTGAT
GCTGGCCCACGTGGTGGAGGGCGAACTCACCCAGGAGGTGCTGATCGGGATCAGCGCGCGGGGGGACGGCAGCCACCTTG
TCCGCCTCGCCTCCTTTGGCGGCCCCATCATCACGCCGGGAGTGAAAGCAGCTGTTGGCGTAGTGACGGACTGGCTGGAA
GAGCAGGGGGCGGCAGTGCGGCACCGAGGGTATTGA

Upstream 100 bases:

>100_bases
GGGTTTACGCGCTGGGCCGCTGTACACCGGGCGACCATACCTACGCTTCGGTGCTGGAGGATGCGGCCCGGCTGGCAGAA
GAACTGGCGGGGGTAGGGGC

Downstream 100 bases:

>100_bases
GGCGGTGCGCCGTTTGCCGTCAGCAGCCATGCCGCAGCCCGAGCTGCACTTTACCTGCGAACCCCTCAGCCGGATTCTGC
CCGCCGTGCGCGCCGCCCTC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIFRLADFHFPDSAARLYPDTPQRPWILEVGFGDGRFWPHYAATFPEAPNYLGVEISGVSLLKAERRLREAGLSNAVLTK
LPATPLIREVVPAGSLDAIVVNFPDPWPKAGHAEHRLLRAPFFRLAASRLKPGGAVLLTTDHDEYFEFACREAEASGVMR
AELTDPPPAALETKYARKWRELGLEVQHARFVPTHHPHVPHGTVARFPDSEDAPAVPHAILTLPAAFDPGAFHKHTARGG
QTREDPVGWTVVLLELYRSLKQDGWVMLAHVVEGELTQEVLIGISARGDGSHLVRLASFGGPIITPGVKAAVGVVTDWLE
EQGAAVRHRGY

Sequences:

>Translated_331_residues
MIFRLADFHFPDSAARLYPDTPQRPWILEVGFGDGRFWPHYAATFPEAPNYLGVEISGVSLLKAERRLREAGLSNAVLTK
LPATPLIREVVPAGSLDAIVVNFPDPWPKAGHAEHRLLRAPFFRLAASRLKPGGAVLLTTDHDEYFEFACREAEASGVMR
AELTDPPPAALETKYARKWRELGLEVQHARFVPTHHPHVPHGTVARFPDSEDAPAVPHAILTLPAAFDPGAFHKHTARGG
QTREDPVGWTVVLLELYRSLKQDGWVMLAHVVEGELTQEVLIGISARGDGSHLVRLASFGGPIITPGVKAAVGVVTDWLE
EQGAAVRHRGY
>Mature_331_residues
MIFRLADFHFPDSAARLYPDTPQRPWILEVGFGDGRFWPHYAATFPEAPNYLGVEISGVSLLKAERRLREAGLSNAVLTK
LPATPLIREVVPAGSLDAIVVNFPDPWPKAGHAEHRLLRAPFFRLAASRLKPGGAVLLTTDHDEYFEFACREAEASGVMR
AELTDPPPAALETKYARKWRELGLEVQHARFVPTHHPHVPHGTVARFPDSEDAPAVPHAILTLPAAFDPGAFHKHTARGG
QTREDPVGWTVVLLELYRSLKQDGWVMLAHVVEGELTQEVLIGISARGDGSHLVRLASFGGPIITPGVKAAVGVVTDWLE
EQGAAVRHRGY

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=123, Percent_Identity=34.1463414634146, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_DEIGD (Q1IWK0)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605553.1
- ProteinModelPortal:   Q1IWK0
- SMR:   Q1IWK0
- STRING:   Q1IWK0
- GeneID:   4058187
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_2090
- NMPDR:   fig|68909.1.peg.2274
- eggNOG:   COG0220
- HOGENOM:   HBG449997
- OMA:   YLGVEIS
- PhylomeDB:   Q1IWK0
- ProtClustDB:   CLSK445190
- BioCyc:   DGEO319795:DGEO_2090-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 36337; Mature: 36337

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 55-55 BINDING 105-105 BINDING 109-109 BINDING 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFRLADFHFPDSAARLYPDTPQRPWILEVGFGDGRFWPHYAATFPEAPNYLGVEISGVS
CEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCEEEEEECCHH
LLKAERRLREAGLSNAVLTKLPATPLIREVVPAGSLDAIVVNFPDPWPKAGHAEHRLLRA
HHHHHHHHHHCCCCCHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
PFFRLAASRLKPGGAVLLTTDHDEYFEFACREAEASGVMRAELTDPPPAALETKYARKWR
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHH
ELGLEVQHARFVPTHHPHVPHGTVARFPDSEDAPAVPHAILTLPAAFDPGAFHKHTARGG
HCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCC
QTREDPVGWTVVLLELYRSLKQDGWVMLAHVVEGELTQEVLIGISARGDGSHLVRLASFG
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHEEEECCCCCHHEEEEECCC
GPIITPGVKAAVGVVTDWLEEQGAAVRHRGY
CCEECCCHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MIFRLADFHFPDSAARLYPDTPQRPWILEVGFGDGRFWPHYAATFPEAPNYLGVEISGVS
CEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCEEEEEECCHH
LLKAERRLREAGLSNAVLTKLPATPLIREVVPAGSLDAIVVNFPDPWPKAGHAEHRLLRA
HHHHHHHHHHCCCCCHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
PFFRLAASRLKPGGAVLLTTDHDEYFEFACREAEASGVMRAELTDPPPAALETKYARKWR
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHH
ELGLEVQHARFVPTHHPHVPHGTVARFPDSEDAPAVPHAILTLPAAFDPGAFHKHTARGG
HCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCC
QTREDPVGWTVVLLELYRSLKQDGWVMLAHVVEGELTQEVLIGISARGDGSHLVRLASFG
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHEEEECCCCCHHEEEEECCC
GPIITPGVKAAVGVVTDWLEEQGAAVRHRGY
CCEECCCHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA