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The map label for this gene is carA

Identifier: 94986159

GI number: 94986159

Start: 2167114

End: 2168298

Strand: Reverse

Name: carA

Synonym: Dgeo_2060

Alternate gene names: 94986159

Gene position: 2168298-2167114 (Counterclockwise)

Preceding gene: 94986160

Following gene: 94986158

Centisome position: 87.88

GC content: 63.29

Gene sequence:

>1185_bases
ATGATTCGTAAGGAACGCGCGATTCTGGCGCTGGAAGACGGTACGGTGTACCGCGGTTATGCTTTTGGCCATCGCGGCGA
GACGGTCGGGGAGGTCGTGTTCAACACCTCCATGACCGGGTACCAGGAGATCATGACCGATCCCTCGTACAACGGGCAGA
TCGTGACCATCACGTACCCGCATGTGGGCAACTACGGCGTGGCCATCTACGACATGGAGAGCAACAAGCCCTACGTGCGA
GGCTTTATCGCGCGCGAGTTTTCCGGCGAGTACTCCAACCACCGCGCGCAACAGTCACTCGAAGCCTTTATGCAGCAGTA
CGGCGTGGTGAGCATCCAAGGGATCGACACGCGGGCGCTGGTGCGGCGTCTTCGCACGGGCGGCGTGGTGAAGGGCGTGA
TCGCTCACCGCTCCTACACGCACCCCGAAGACCCCTACGGCGAGTTCACCCCCGCTGAGGAGCAGATCTACGTGCAGCGT
GCCCGCGACCATCAGGACATCGACGGGCACGACATGACGAAGGAGGTCACCACACCGCTGCCCTACGCCTTCCCGACCCT
GCGCCACGGCAAGCGCGTGGTGTTGATGGATTTCGGCATCAAGCACACCATCATCGAACGGCTGGCGGAGGTGGGCATTG
AACCCATTGTGGTGCCAGCACACACCACCCCGGCGCAGATCATGGCCCTCCAGCCGCACGGCCTCTTTTTGTCGAACGGC
CCCGGGGACCCCGCTCCACTGGAATACGCGCACAAGACCGCCTGGGAACTGATGGGTCTGCTGCCCACCTTTGGCATCTG
CTTGGGCCACCAGATTCTGGGTCTTGCGGCGGGCGGCCAGACCTTCAAGATGAAGTTCGGGCACCGCGGAGGCAACCAGC
CGGTGAAGAACCTGCTCACCGGGAATGTGGAGATCACCTCGCAGAACCACGGCTACGCGGTGGACATCGCCTCCATCCCG
GACGGCGCCTTTGTCGCCACCCACGTCAACCTCAACGACGGCACGCTAGAAGGCATGGCTCACAGCCGCTATCCGGTCTT
CTCGGTGCAGTACCACCCCGAGGCGTCCCCCGGTCCCCACGACAGCCGCTACCTCTTTGACCGCTTTATCGAGGAGATCG
ATGCCTTCGAGGGCGCAAACGGTTCGCCGGTCCTGAAGGCTTCGGCGGGGCGGCTGGGGGTGTAG

Upstream 100 bases:

>100_bases
CCGTGGTCAGGCTTGCTAGGATTCACGGCTGAAAGCTGCGGCTGACCGCTGCCAGCTTCCGCCATGAACTGCGTCCCGTG
AGGCGCGAATGGAGGTTTCT

Downstream 100 bases:

>100_bases
GGAGCTTTCCGCTCCACTTCCACTTGAGCGTTCCTTGAGGCGAGAGCGCCTTCCCTGCCGGGAGCGGGAGACTGAAGCTC
GCCCATGAGCGACCCTGCCT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MIRKERAILALEDGTVYRGYAFGHRGETVGEVVFNTSMTGYQEIMTDPSYNGQIVTITYPHVGNYGVAIYDMESNKPYVR
GFIAREFSGEYSNHRAQQSLEAFMQQYGVVSIQGIDTRALVRRLRTGGVVKGVIAHRSYTHPEDPYGEFTPAEEQIYVQR
ARDHQDIDGHDMTKEVTTPLPYAFPTLRHGKRVVLMDFGIKHTIIERLAEVGIEPIVVPAHTTPAQIMALQPHGLFLSNG
PGDPAPLEYAHKTAWELMGLLPTFGICLGHQILGLAAGGQTFKMKFGHRGGNQPVKNLLTGNVEITSQNHGYAVDIASIP
DGAFVATHVNLNDGTLEGMAHSRYPVFSVQYHPEASPGPHDSRYLFDRFIEEIDAFEGANGSPVLKASAGRLGV

Sequences:

>Translated_394_residues
MIRKERAILALEDGTVYRGYAFGHRGETVGEVVFNTSMTGYQEIMTDPSYNGQIVTITYPHVGNYGVAIYDMESNKPYVR
GFIAREFSGEYSNHRAQQSLEAFMQQYGVVSIQGIDTRALVRRLRTGGVVKGVIAHRSYTHPEDPYGEFTPAEEQIYVQR
ARDHQDIDGHDMTKEVTTPLPYAFPTLRHGKRVVLMDFGIKHTIIERLAEVGIEPIVVPAHTTPAQIMALQPHGLFLSNG
PGDPAPLEYAHKTAWELMGLLPTFGICLGHQILGLAAGGQTFKMKFGHRGGNQPVKNLLTGNVEITSQNHGYAVDIASIP
DGAFVATHVNLNDGTLEGMAHSRYPVFSVQYHPEASPGPHDSRYLFDRFIEEIDAFEGANGSPVLKASAGRLGV
>Mature_394_residues
MIRKERAILALEDGTVYRGYAFGHRGETVGEVVFNTSMTGYQEIMTDPSYNGQIVTITYPHVGNYGVAIYDMESNKPYVR
GFIAREFSGEYSNHRAQQSLEAFMQQYGVVSIQGIDTRALVRRLRTGGVVKGVIAHRSYTHPEDPYGEFTPAEEQIYVQR
ARDHQDIDGHDMTKEVTTPLPYAFPTLRHGKRVVLMDFGIKHTIIERLAEVGIEPIVVPAHTTPAQIMALQPHGLFLSNG
PGDPAPLEYAHKTAWELMGLLPTFGICLGHQILGLAAGGQTFKMKFGHRGGNQPVKNLLTGNVEITSQNHGYAVDIASIP
DGAFVATHVNLNDGTLEGMAHSRYPVFSVQYHPEASPGPHDSRYLFDRFIEEIDAFEGANGSPVLKASAGRLGV

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=390, Percent_Identity=38.2051282051282, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI21361331, Length=402, Percent_Identity=36.0696517412935, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI169790915, Length=402, Percent_Identity=36.0696517412935, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1786215, Length=388, Percent_Identity=44.8453608247423, Blast_Score=322, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI193204318, Length=386, Percent_Identity=39.8963730569948, Blast_Score=240, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=389, Percent_Identity=38.3033419023136, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=37.5634517766497, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=38.7596899224806, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=38.7596899224806, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_DEIGD (Q1IWN0)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605523.1
- ProteinModelPortal:   Q1IWN0
- SMR:   Q1IWN0
- STRING:   Q1IWN0
- GeneID:   4058406
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_2060
- NMPDR:   fig|68909.1.peg.2245
- eggNOG:   COG0505
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- PhylomeDB:   Q1IWN0
- ProtClustDB:   PRK12564
- BioCyc:   DGEO319795:DGEO_2060-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 43392; Mature: 43392

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 267-267 ACT_SITE 352-352 ACT_SITE 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKERAILALEDGTVYRGYAFGHRGETVGEVVFNTSMTGYQEIMTDPSYNGQIVTITYP
CCCCCCEEEEEECCCEEEEEEECCCCCCHHHHEECCCCCCHHHHHCCCCCCCEEEEEECC
HVGNYGVAIYDMESNKPYVRGFIAREFSGEYSNHRAQQSLEAFMQQYGVVSIQGIDTRAL
CCCCCCEEEEEECCCCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
VRRLRTGGVVKGVIAHRSYTHPEDPYGEFTPAEEQIYVQRARDHQDIDGHDMTKEVTTPL
HHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCC
PYAFPTLRHGKRVVLMDFGIKHTIIERLAEVGIEPIVVPAHTTPAQIMALQPHGLFLSNG
CCCCCHHHCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEECC
PGDPAPLEYAHKTAWELMGLLPTFGICLGHQILGLAAGGQTFKMKFGHRGGNQPVKNLLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCHHHHHHC
GNVEITSQNHGYAVDIASIPDGAFVATHVNLNDGTLEGMAHSRYPVFSVQYHPEASPGPH
CCEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCC
DSRYLFDRFIEEIDAFEGANGSPVLKASAGRLGV
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MIRKERAILALEDGTVYRGYAFGHRGETVGEVVFNTSMTGYQEIMTDPSYNGQIVTITYP
CCCCCCEEEEEECCCEEEEEEECCCCCCHHHHEECCCCCCHHHHHCCCCCCCEEEEEECC
HVGNYGVAIYDMESNKPYVRGFIAREFSGEYSNHRAQQSLEAFMQQYGVVSIQGIDTRAL
CCCCCCEEEEEECCCCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
VRRLRTGGVVKGVIAHRSYTHPEDPYGEFTPAEEQIYVQRARDHQDIDGHDMTKEVTTPL
HHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCC
PYAFPTLRHGKRVVLMDFGIKHTIIERLAEVGIEPIVVPAHTTPAQIMALQPHGLFLSNG
CCCCCHHHCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEECC
PGDPAPLEYAHKTAWELMGLLPTFGICLGHQILGLAAGGQTFKMKFGHRGGNQPVKNLLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCHHHHHHC
GNVEITSQNHGYAVDIASIPDGAFVATHVNLNDGTLEGMAHSRYPVFSVQYHPEASPGPH
CCEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCC
DSRYLFDRFIEEIDAFEGANGSPVLKASAGRLGV
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA