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The map label for this gene is nrgA [H]

Identifier: 94986153

GI number: 94986153

Start: 2160859

End: 2162154

Strand: Reverse

Name: nrgA [H]

Synonym: Dgeo_2054

Alternate gene names: 94986153

Gene position: 2162154-2160859 (Counterclockwise)

Preceding gene: 94986154

Following gene: 94986152

Centisome position: 87.64

GC content: 67.52

Gene sequence:

>1296_bases
ATGAAGCGGCTCCCTCTGCTGGTTTTGCTGAGTGGCGCGGCCCTGGCGCAGTCGGCCACACCTACCCTGGACACAGGCGA
CACCGCTTGGATGCTGATCGCCGCCGCCCTCGTTCTGCTGATGACCCCCGGCCTGGCCTTTTTCTACGGCGGCCTCACCC
GTGCGGGCAGCGTGCTGAACACCATGATGATGAGCGTGGCGTCGCTGGGGTTGGTTGGCGTGCTGTGGACCCTGGCGGGC
TATACCCTGGCCTTCGGTTCGGGCGGCAATGCGCTGATCGGCGGCCTCGCGAACCTGGGTCTAGCCGGGGTGCCAGGTGC
TCTCTGGGGGACTATCCCCAGTTCGGTCTTCGCCGCGTTCCAGGCGATGTTCGCGATCATCGCCCTCGCCCTCATCAGCG
GCGCAGTGGTGGAGCGCATGCGCTTTGGCGCGTTTCTTTGTTTCGGGGGGCTGTGGAGCCTGCTGATCTATGCCCCACTG
GCGCACTGGGTCTGGAGTGCGGACGGCTGGCTCTACAAACTGGGTGCCTTGGATTTCGCGGGCGGAACCGTGATTCATAT
CGCGGCGGGGGTGAGCGCGTTGGTGGCTGCCGCCGTCCTGGGGCCACGGCTGGGCTATCCCCGTGCCGTGCACGTGCCGC
ACAACGTCCCCTTTGTGCTGCTGGGGGCTGGGCTGCTGTGGTTTGGCTGGATCGGTTTCAATGCCGGAAGCGCGCTGGCG
GCCAACCAGACCGCCGGGCTGGCCTTGCTCACCACGCTGGTCGCTCCCGCTGCCGCCCTGCTCACTTGGCTGGCTTGGGA
AAGCCGCGGTGAAGGCAAACCCACGGCCGTCGGCGCGGCAACCGGCCTGGTGGTGGGGCTGGTCGCCATCACGCCCGCCT
GCGCCTTTGTGACCCCCTGGGCGGCCCTCGTGATCGGTGTGCTGGGGGCAAGCGCGAGCTTCTGGACCGTGCAGGCCAAG
CAGCGACTACGCGCCGATGACGCCCTCGATGTCTTCGCCTGCCATGGCGTTGCCGGAATTGTGGGTGCGCTGTTGACGGG
GGCGCTCGCCTGGACGACCGGCAGTGGGAAGCCGCTGGGTGAGCAACTCCTGATCCAGGTGCTCGGGGTGCTGGCGAGCG
TGCTGTTTACTGGTCTGGGCAGCTTCCTCCTGCTGAAACTCGTCAGCGTGCTGATGCCGCTGCGGGTGTCGGCGCGGCAG
GAGGTGGGCGGCATGGATCTCAGCGCCCACAGCGAGCAGGGCTACAGCGAACAGGAAACGGGTCTGGGCGCCCCGGTGTT
TGTGGGCGGCGACTAA

Upstream 100 bases:

>100_bases
AACGGGTGGTCCGCATCCGCACCGGCGAGGAGGACAACGCGGCCCTCACGCCCGTCACCGAGACGAGGCTCACGCCGGGC
CTGCCTGTGAGGCACGCGCG

Downstream 100 bases:

>100_bases
AAATAAAAAAGTTCTTCTGGTCCCGGTCCATCCTGCTTGCCTGGGGTGGACCGGGACGCTTGCCCTCCGGTGCTTCCATG
AACGCTGTGTTAGGATGTTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKRLPLLVLLSGAALAQSATPTLDTGDTAWMLIAAALVLLMTPGLAFFYGGLTRAGSVLNTMMMSVASLGLVGVLWTLAG
YTLAFGSGGNALIGGLANLGLAGVPGALWGTIPSSVFAAFQAMFAIIALALISGAVVERMRFGAFLCFGGLWSLLIYAPL
AHWVWSADGWLYKLGALDFAGGTVIHIAAGVSALVAAAVLGPRLGYPRAVHVPHNVPFVLLGAGLLWFGWIGFNAGSALA
ANQTAGLALLTTLVAPAAALLTWLAWESRGEGKPTAVGAATGLVVGLVAITPACAFVTPWAALVIGVLGASASFWTVQAK
QRLRADDALDVFACHGVAGIVGALLTGALAWTTGSGKPLGEQLLIQVLGVLASVLFTGLGSFLLLKLVSVLMPLRVSARQ
EVGGMDLSAHSEQGYSEQETGLGAPVFVGGD

Sequences:

>Translated_431_residues
MKRLPLLVLLSGAALAQSATPTLDTGDTAWMLIAAALVLLMTPGLAFFYGGLTRAGSVLNTMMMSVASLGLVGVLWTLAG
YTLAFGSGGNALIGGLANLGLAGVPGALWGTIPSSVFAAFQAMFAIIALALISGAVVERMRFGAFLCFGGLWSLLIYAPL
AHWVWSADGWLYKLGALDFAGGTVIHIAAGVSALVAAAVLGPRLGYPRAVHVPHNVPFVLLGAGLLWFGWIGFNAGSALA
ANQTAGLALLTTLVAPAAALLTWLAWESRGEGKPTAVGAATGLVVGLVAITPACAFVTPWAALVIGVLGASASFWTVQAK
QRLRADDALDVFACHGVAGIVGALLTGALAWTTGSGKPLGEQLLIQVLGVLASVLFTGLGSFLLLKLVSVLMPLRVSARQ
EVGGMDLSAHSEQGYSEQETGLGAPVFVGGD
>Mature_431_residues
MKRLPLLVLLSGAALAQSATPTLDTGDTAWMLIAAALVLLMTPGLAFFYGGLTRAGSVLNTMMMSVASLGLVGVLWTLAG
YTLAFGSGGNALIGGLANLGLAGVPGALWGTIPSSVFAAFQAMFAIIALALISGAVVERMRFGAFLCFGGLWSLLIYAPL
AHWVWSADGWLYKLGALDFAGGTVIHIAAGVSALVAAAVLGPRLGYPRAVHVPHNVPFVLLGAGLLWFGWIGFNAGSALA
ANQTAGLALLTTLVAPAAALLTWLAWESRGEGKPTAVGAATGLVVGLVAITPACAFVTPWAALVIGVLGASASFWTVQAK
QRLRADDALDVFACHGVAGIVGALLTGALAWTTGSGKPLGEQLLIQVLGVLASVLFTGLGSFLLLKLVSVLMPLRVSARQ
EVGGMDLSAHSEQGYSEQETGLGAPVFVGGD

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=411, Percent_Identity=43.0656934306569, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17550182, Length=450, Percent_Identity=29.3333333333333, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17550180, Length=453, Percent_Identity=27.5938189845475, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=27.5058275058275, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=452, Percent_Identity=25, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=444, Percent_Identity=35.8108108108108, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6325396, Length=421, Percent_Identity=35.1543942992874, Blast_Score=226, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=33.890214797136, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI161078314, Length=412, Percent_Identity=32.5242718446602, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43858; Mature: 43858

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLPLLVLLSGAALAQSATPTLDTGDTAWMLIAAALVLLMTPGLAFFYGGLTRAGSVLN
CCCCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TMMMSVASLGLVGVLWTLAGYTLAFGSGGNALIGGLANLGLAGVPGALWGTIPSSVFAAF
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
QAMFAIIALALISGAVVERMRFGAFLCFGGLWSLLIYAPLAHWVWSADGWLYKLGALDFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECC
GGTVIHIAAGVSALVAAAVLGPRLGYPRAVHVPHNVPFVLLGAGLLWFGWIGFNAGSALA
CCCCEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ANQTAGLALLTTLVAPAAALLTWLAWESRGEGKPTAVGAATGLVVGLVAITPACAFVTPW
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALVIGVLGASASFWTVQAKQRLRADDALDVFACHGVAGIVGALLTGALAWTTGSGKPLG
HHHHHHHHCCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHH
EQLLIQVLGVLASVLFTGLGSFLLLKLVSVLMPLRVSARQEVGGMDLSAHSEQGYSEQET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHC
GLGAPVFVGGD
CCCCCEEECCC
>Mature Secondary Structure
MKRLPLLVLLSGAALAQSATPTLDTGDTAWMLIAAALVLLMTPGLAFFYGGLTRAGSVLN
CCCCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TMMMSVASLGLVGVLWTLAGYTLAFGSGGNALIGGLANLGLAGVPGALWGTIPSSVFAAF
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
QAMFAIIALALISGAVVERMRFGAFLCFGGLWSLLIYAPLAHWVWSADGWLYKLGALDFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECC
GGTVIHIAAGVSALVAAAVLGPRLGYPRAVHVPHNVPFVLLGAGLLWFGWIGFNAGSALA
CCCCEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ANQTAGLALLTTLVAPAAALLTWLAWESRGEGKPTAVGAATGLVVGLVAITPACAFVTPW
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALVIGVLGASASFWTVQAKQRLRADDALDVFACHGVAGIVGALLTGALAWTTGSGKPLG
HHHHHHHHCCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHH
EQLLIQVLGVLASVLFTGLGSFLLLKLVSVLMPLRVSARQEVGGMDLSAHSEQGYSEQET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHC
GLGAPVFVGGD
CCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]