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The map label for this gene is dusA [H]

Identifier: 94986095

GI number: 94986095

Start: 2097811

End: 2098845

Strand: Reverse

Name: dusA [H]

Synonym: Dgeo_1996

Alternate gene names: 94986095

Gene position: 2098845-2097811 (Counterclockwise)

Preceding gene: 94986097

Following gene: 94986092

Centisome position: 85.07

GC content: 68.5

Gene sequence:

>1035_bases
ATGACGGCTGCTTTCCGCCCGCCCCATACCTTTTCGGTCGCTCCGATGATGGACTGGACCGACCGGCACTGCCGCGCGTT
TCACCGCACGCTGACGCGCCGGACGCTGCTCTATACCGAGATGGTCACGACCGGGGCCATTTTGCACGGTGACCGCGCGC
GGCACCTGGGGTTCAGTCCGGCTGAACATCCGGTTGCCCTGCAACTGGGCGGCAGCGATCCGGCGGCGCTGGCCGAGTGT
GCCCGGATTGCCCAGGACTGGGGTTATGACGAGGTGAACCTCAACTGCGGTTGCCCCAGCGACCGAGTGCAAAACGGCTC
GTTCGGGGCTTGCCTGATGGCGACACCGGACGTGGTGGCCCGCGCGGTGGAGGCCATGCGGGGGGCAACCACCCTCCCCG
TTACGGTGAAACACCGCATCGGCATTGACGACCTGGACAGCTACGAGCACCTGACGCGCTTTGTGCGGACGGTGGCGGAG
GCGGGCTGTCAGACCTTTATCGTCCATGCCCGCAAGGCCTGGCTCTCCGGCCTGTCCCCTAAGGAGAACCGGGAAATTCC
CCCGCTGCGCTACGAGGTGGTGCGCCAGCTCAAGGCCGACTTCCCGCACCTGACCGTCGTGCTGAATGGCGGTGTGCTGA
CGCTGGCGGCGGCGCAGGAGCATCTGGCCTGGGCCGACGGCGTGATGGTGGGCCGCGCCGCCTATCAGGATCCTTACCTC
TTGGCTGCCGCCGACCGCGACCTGTTCGGCGAGGACGTCACCCCGCCTACCCGCCGCGAAGCGATCGAGGCGTTCCTGCC
CTACGTCGCTGCACAGCTGGCAGAGGGCCAGCCGCTGCACCGCATGATGCGGCATACCCTGGGGCTGTTTGCGGGTCAGC
CCGGCGCTCGTCACTGGAAACGGACGCTGAGCGAGCGGGGCCACCGCCCCGGCGCGGGGCTGGAGGTGGTGCGGGAAGCG
CTGGCGGGGGTGCCGGAGAGTGTGTTGGACGCGCGGCCCGTGGTGGGGGAGAGTCAGGCGCTGGAAGCAAGATGA

Upstream 100 bases:

>100_bases
ACGCTGCCGTTGCACCCTCTCGGGCGCTGGACCCTTGCGCTGCTCAGCTGGACGCCTCCCCACCTTTTGTTCTTGACGCT
TTCCGTACACTGACCGGCTG

Downstream 100 bases:

>100_bases
AACGGCCCCTCTCCTTCATGGAAAGGGGCTGAGCCTGGTGGTGGCGCTCAAGGGTATTTCAGAAGCGGTAGCCGACGCTG
AGGCCAAAGGCAGGCAAGAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTAAFRPPHTFSVAPMMDWTDRHCRAFHRTLTRRTLLYTEMVTTGAILHGDRARHLGFSPAEHPVALQLGGSDPAALAEC
ARIAQDWGYDEVNLNCGCPSDRVQNGSFGACLMATPDVVARAVEAMRGATTLPVTVKHRIGIDDLDSYEHLTRFVRTVAE
AGCQTFIVHARKAWLSGLSPKENREIPPLRYEVVRQLKADFPHLTVVLNGGVLTLAAAQEHLAWADGVMVGRAAYQDPYL
LAAADRDLFGEDVTPPTRREAIEAFLPYVAAQLAEGQPLHRMMRHTLGLFAGQPGARHWKRTLSERGHRPGAGLEVVREA
LAGVPESVLDARPVVGESQALEAR

Sequences:

>Translated_344_residues
MTAAFRPPHTFSVAPMMDWTDRHCRAFHRTLTRRTLLYTEMVTTGAILHGDRARHLGFSPAEHPVALQLGGSDPAALAEC
ARIAQDWGYDEVNLNCGCPSDRVQNGSFGACLMATPDVVARAVEAMRGATTLPVTVKHRIGIDDLDSYEHLTRFVRTVAE
AGCQTFIVHARKAWLSGLSPKENREIPPLRYEVVRQLKADFPHLTVVLNGGVLTLAAAQEHLAWADGVMVGRAAYQDPYL
LAAADRDLFGEDVTPPTRREAIEAFLPYVAAQLAEGQPLHRMMRHTLGLFAGQPGARHWKRTLSERGHRPGAGLEVVREA
LAGVPESVLDARPVVGESQALEAR
>Mature_343_residues
TAAFRPPHTFSVAPMMDWTDRHCRAFHRTLTRRTLLYTEMVTTGAILHGDRARHLGFSPAEHPVALQLGGSDPAALAECA
RIAQDWGYDEVNLNCGCPSDRVQNGSFGACLMATPDVVARAVEAMRGATTLPVTVKHRIGIDDLDSYEHLTRFVRTVAEA
GCQTFIVHARKAWLSGLSPKENREIPPLRYEVVRQLKADFPHLTVVLNGGVLTLAAAQEHLAWADGVMVGRAAYQDPYLL
AAADRDLFGEDVTPPTRREAIEAFLPYVAAQLAEGQPLHRMMRHTLGLFAGQPGARHWKRTLSERGHRPGAGLEVVREAL
AGVPESVLDARPVVGESQALEAR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=301, Percent_Identity=25.5813953488372, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI8923374, Length=192, Percent_Identity=27.6041666666667, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=61.0062893081761, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1788462, Length=186, Percent_Identity=31.7204301075269, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789660, Length=248, Percent_Identity=26.2096774193548, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=279, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=27.7310924369748, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24580595, Length=241, Percent_Identity=24.896265560166, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920448, Length=241, Percent_Identity=24.896265560166, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37697; Mature: 37566

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAFRPPHTFSVAPMMDWTDRHCRAFHRTLTRRTLLYTEMVTTGAILHGDRARHLGFSP
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCCC
AEHPVALQLGGSDPAALAECARIAQDWGYDEVNLNCGCPSDRVQNGSFGACLMATPDVVA
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCEEEECCHHHHH
RAVEAMRGATTLPVTVKHRIGIDDLDSYEHLTRFVRTVAEAGCQTFIVHARKAWLSGLSP
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
KENREIPPLRYEVVRQLKADFPHLTVVLNGGVLTLAAAQEHLAWADGVMVGRAAYQDPYL
CCCCCCCCHHHHHHHHHHCCCCEEEEEEECCEEEEHHHHHHHHHHCCHHCCHHHCCCCEE
LAAADRDLFGEDVTPPTRREAIEAFLPYVAAQLAEGQPLHRMMRHTLGLFAGQPGARHWK
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
RTLSERGHRPGAGLEVVREALAGVPESVLDARPVVGESQALEAR
HHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TAAFRPPHTFSVAPMMDWTDRHCRAFHRTLTRRTLLYTEMVTTGAILHGDRARHLGFSP
CCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCCC
AEHPVALQLGGSDPAALAECARIAQDWGYDEVNLNCGCPSDRVQNGSFGACLMATPDVVA
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCEEEECCHHHHH
RAVEAMRGATTLPVTVKHRIGIDDLDSYEHLTRFVRTVAEAGCQTFIVHARKAWLSGLSP
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
KENREIPPLRYEVVRQLKADFPHLTVVLNGGVLTLAAAQEHLAWADGVMVGRAAYQDPYL
CCCCCCCCHHHHHHHHHHCCCCEEEEEEECCEEEEHHHHHHHHHHCCHHCCHHHCCCCEE
LAAADRDLFGEDVTPPTRREAIEAFLPYVAAQLAEGQPLHRMMRHTLGLFAGQPGARHWK
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
RTLSERGHRPGAGLEVVREALAGVPESVLDARPVVGESQALEAR
HHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]