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The map label for this gene is rpoA

Identifier: 94985940

GI number: 94985940

Start: 1951169

End: 1952164

Strand: Reverse

Name: rpoA

Synonym: Dgeo_1840

Alternate gene names: 94985940

Gene position: 1952164-1951169 (Counterclockwise)

Preceding gene: 94985941

Following gene: 94985939

Centisome position: 79.12

GC content: 63.05

Gene sequence:

>996_bases
GTGGATCAAAAGCGCCCTCAACTCAAGGCCCGCGTGGAGGGAGATTACGGCGAGTTCGTCCTCGAACCGCTCACGCGCGG
CTACGGCGTCACCATCGGGAATCCCATCCGGCGCATCCTGATGTCCTCGATTCCCGGTACTGCCGTGACGAGCGTCTACA
TCGAGGATGTCCTGCACGAGTTTTCCACTATCCCCGGCGTCAAGGAAGACGTCATCCGCATCATCCTGAATCTCAAGGAA
CTTGTGGTGAAATTCCACGCGCCTGGCCCGAAGACCCTGACACTGCGGGCGCAGGGTGAGGGTGTGGTCAAGGCCAGTGC
CTTTGAGGTTCCAAGTGATGCCGAGATCGTCAACCCCGACCTGACGATCGCCACCCTCGCGGAGGATGGCAAGCTGGTCA
TGGAGGTGCGCGTCGAGGAGGGCGAGGGCTATGTCCCGGCCGACAAGCACGCCACCAAGGACCGCATCAACTCGATCCCG
GTGGACGCTGTGTTTTCTCCGGTTCGGCGCGTGGCTTATCACGTTGAGAACACTCGTGTGGGCCAGCAGACCGACCTGGA
CCGCTTGATTCTGCGGATCTGGACAGATGGCAGTGTCGGCCCCCAGGAGGCGCTGGACCGGGCGGTGGAGATTCTGCGTG
ATGAGCTGACCGTGTTCGGCAATGTCGAGACGGTGTCGGCTGCTGCACCCGAATTGCCGTCGGTCTATACGCCTGCTGCT
CCCATCGTGAGTGGCTACGACCTGCCGCGCCAGCCCGAACTCAGCATCAACCCCCAGCCGTACCCCGCTGACCTCGACAC
GCCCCGCGTGACGCTGGAAGGCTTGGGCCTCACCACCCGAGTGCTGCACTCGCTCAAGGAAGAAGGCATCGACTCGGTGG
ACGCCCTGTGCGCCCTCTCCGACCGTGACCTGAAGAAGGTGCCTGGCATCGGTGAGCGCAGCCTGGATGAGATCAAGCAG
CAGTTGGCCCAGTTCGGCCTGGCGCTGCGGGACTGA

Upstream 100 bases:

>100_bases
ACCGGCACCTTCGTGCGCCTGCCTGCGCGCGAGGATCTCGCACTGCCGATCAACGAGAACTTCATCATCGAGTATTACTC
GCGCTAATCGGGAGGCCCCA

Downstream 100 bases:

>100_bases
GCCGGAGGTGGGGGGCAACCCCCCCATCCACGCGGAACACAAAGACGAGCAAGGGCGGTCTCAGGCCAGCAGCGTGTGGT
GATTTTTCTGCCCTACCGCC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MDQKRPQLKARVEGDYGEFVLEPLTRGYGVTIGNPIRRILMSSIPGTAVTSVYIEDVLHEFSTIPGVKEDVIRIILNLKE
LVVKFHAPGPKTLTLRAQGEGVVKASAFEVPSDAEIVNPDLTIATLAEDGKLVMEVRVEEGEGYVPADKHATKDRINSIP
VDAVFSPVRRVAYHVENTRVGQQTDLDRLILRIWTDGSVGPQEALDRAVEILRDELTVFGNVETVSAAAPELPSVYTPAA
PIVSGYDLPRQPELSINPQPYPADLDTPRVTLEGLGLTTRVLHSLKEEGIDSVDALCALSDRDLKKVPGIGERSLDEIKQ
QLAQFGLALRD

Sequences:

>Translated_331_residues
MDQKRPQLKARVEGDYGEFVLEPLTRGYGVTIGNPIRRILMSSIPGTAVTSVYIEDVLHEFSTIPGVKEDVIRIILNLKE
LVVKFHAPGPKTLTLRAQGEGVVKASAFEVPSDAEIVNPDLTIATLAEDGKLVMEVRVEEGEGYVPADKHATKDRINSIP
VDAVFSPVRRVAYHVENTRVGQQTDLDRLILRIWTDGSVGPQEALDRAVEILRDELTVFGNVETVSAAAPELPSVYTPAA
PIVSGYDLPRQPELSINPQPYPADLDTPRVTLEGLGLTTRVLHSLKEEGIDSVDALCALSDRDLKKVPGIGERSLDEIKQ
QLAQFGLALRD
>Mature_331_residues
MDQKRPQLKARVEGDYGEFVLEPLTRGYGVTIGNPIRRILMSSIPGTAVTSVYIEDVLHEFSTIPGVKEDVIRIILNLKE
LVVKFHAPGPKTLTLRAQGEGVVKASAFEVPSDAEIVNPDLTIATLAEDGKLVMEVRVEEGEGYVPADKHATKDRINSIP
VDAVFSPVRRVAYHVENTRVGQQTDLDRLILRIWTDGSVGPQEALDRAVEILRDELTVFGNVETVSAAAPELPSVYTPAA
PIVSGYDLPRQPELSINPQPYPADLDTPRVTLEGLGLTTRVLHSLKEEGIDSVDALCALSDRDLKKVPGIGERSLDEIKQ
QLAQFGLALRD

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=324, Percent_Identity=39.1975308641975, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_DEIGD (Q1IX99)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605304.1
- ProteinModelPortal:   Q1IX99
- STRING:   Q1IX99
- GeneID:   4057570
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_1840
- NMPDR:   fig|68909.1.peg.216
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   DGEO319795:DGEO_1840-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36196; Mature: 36196

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKRPQLKARVEGDYGEFVLEPLTRGYGVTIGNPIRRILMSSIPGTAVTSVYIEDVLHE
CCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSTIPGVKEDVIRIILNLKELVVKFHAPGPKTLTLRAQGEGVVKASAFEVPSDAEIVNPD
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCEEECCC
LTIATLAEDGKLVMEVRVEEGEGYVPADKHATKDRINSIPVDAVFSPVRRVAYHVENTRV
CEEEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
GQQTDLDRLILRIWTDGSVGPQEALDRAVEILRDELTVFGNVETVSAAAPELPSVYTPAA
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCC
PIVSGYDLPRQPELSINPQPYPADLDTPRVTLEGLGLTTRVLHSLKEEGIDSVDALCALS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHCC
DRDLKKVPGIGERSLDEIKQQLAQFGLALRD
CCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDQKRPQLKARVEGDYGEFVLEPLTRGYGVTIGNPIRRILMSSIPGTAVTSVYIEDVLHE
CCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSTIPGVKEDVIRIILNLKELVVKFHAPGPKTLTLRAQGEGVVKASAFEVPSDAEIVNPD
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCEEECCC
LTIATLAEDGKLVMEVRVEEGEGYVPADKHATKDRINSIPVDAVFSPVRRVAYHVENTRV
CEEEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
GQQTDLDRLILRIWTDGSVGPQEALDRAVEILRDELTVFGNVETVSAAAPELPSVYTPAA
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCC
PIVSGYDLPRQPELSINPQPYPADLDTPRVTLEGLGLTTRVLHSLKEEGIDSVDALCALS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHCC
DRDLKKVPGIGERSLDEIKQQLAQFGLALRD
CCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA