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The map label for this gene is radA [H]

Identifier: 94985314

GI number: 94985314

Start: 1291648

End: 1292997

Strand: Reverse

Name: radA [H]

Synonym: Dgeo_1212

Alternate gene names: 94985314

Gene position: 1292997-1291648 (Counterclockwise)

Preceding gene: 94985315

Following gene: 94985313

Centisome position: 52.41

GC content: 68.96

Gene sequence:

>1350_bases
GTGGCTAAGGTCACGACAAGATACGTTTGTACGTCCTGCGGCTACCAGTCCGCCAAGCCGCTGGGCCGCTGCCCCAACTG
CCAGGCGTGGAACTCTTTTGAAGAGGAGCTGCCTGCGGCTGTGCCGGGCAAGACCCGGATGGGTGGGAGTGGGGGCTACG
GCGGCGTGGTCGGCGGAAAGCTAACCCCCCTCTCCACCGTCGGGCGCCGCGAGGAACCTCGCCTGCCCAGCGGGATTCCC
GAACTTGACCGGGTGCTGGGCGGCGGGCTGGTCGCGGGCAGCGTGACCCTGATCGGCGGCGAACCCGGCATCGGCAAGAG
CACGCTGCTGTTGCAGGTGGCCGACCGGCTTGCGCGGAATGGCCAGACGGTGCTGTACGTGGCGGGTGAGGAGTCGCTCG
AACAGATTCGCCTGCGCGCCGACCGCCTGAACGTGACCGCTGAGATTCAACTCACCCGCGACACCCGCGCCGAACATATC
GCCGCGTTGATGCAGGAGCACCGACCCGCCCTGTGCATCGTGGACTCCATCCAGACCGTGACGGTGGAGGCCGAGGGCGC
GCCCGGCGGGGTCGCTCAGGTGCGCGAGGGAACGGCCCTGCTCACCCGCGCAGCCAAGGAAACCGGAACGGCGACGGTCC
TGGTCGGCCACGTGACCAAGGAAGGCACGGTTGCCGGGCCAAAGGTCATGGAACATATCGTGGATACCACCGTGTTTCTC
GAGACGGTGGGCGCTTTCCGCCTGCTCAGGAGCGTGAAAAACCGCTTCGGACAAGCGGGCGAACTCGGTGTATTCGAGAT
GCGTGGGGAAGGACTGGTCGCGGTCGAGAACCCCAGCGCCGCCTTCTTGGCCGAGCGGCCTGTGGGGGTTCCGGGGAGTG
TCGTCGCCGCGACCATTGACGGCCAGCGGCCTATGTTGTTGGAGGTGCAGGCGTTGGCCGCCAAGACGCCCTATCCCAAT
CCACGCCGCGTGGTGGTCGGCCTCGATCCCCGGCGCGTCGATGTGGTGCTGGCGGTGCTGGAACGCCGCCTCGACCTCAC
GTTGGGCGGGCTGGACGTGTACGTGAACCTGGCAGGCGGCCTGAAGGTGCCGGACCCCGGCCTCGACCTCCCGGTCGCGC
TCGCCGTGTACTCGGCGGTCGTGGGCCGGGCGCTGCCGGGCAATGTCGCCGTCTTTGGGGAAGTTGGGCTGGCGGGGGAG
GTGCGTTCCACCCAGGGTGCCTTGCGCCGCGCGGAGGAAGCCGCCCGCGCTGGGTACCGCAGCCTGATTGTGCCGCCCGG
CCTGGAGGGGCGCGAGGGCGTCCGCAGCGTGGAGGAGGCGGTCGGTCTGGTGTGGCAGCCCAATCCCTGA

Upstream 100 bases:

>100_bases
CCAGGCCTGCTCCTGACTGAACTTGTGGACGGGGACTATCTCGCGGCGCGGGACGGCACCTACTGGCGCGCAGACAGCAG
GCCCGAGCCTCAGGCATACC

Downstream 100 bases:

>100_bases
AAGAGGGAGACTCAACTGTTGATAGAGGATCGCTCAAGAAAAGACCCGGCCTCTGAAATGTGCTTGCCAAAATTCTGAGG
CACACTGGCCTCACCATGAC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAKVTTRYVCTSCGYQSAKPLGRCPNCQAWNSFEEELPAAVPGKTRMGGSGGYGGVVGGKLTPLSTVGRREEPRLPSGIP
ELDRVLGGGLVAGSVTLIGGEPGIGKSTLLLQVADRLARNGQTVLYVAGEESLEQIRLRADRLNVTAEIQLTRDTRAEHI
AALMQEHRPALCIVDSIQTVTVEAEGAPGGVAQVREGTALLTRAAKETGTATVLVGHVTKEGTVAGPKVMEHIVDTTVFL
ETVGAFRLLRSVKNRFGQAGELGVFEMRGEGLVAVENPSAAFLAERPVGVPGSVVAATIDGQRPMLLEVQALAAKTPYPN
PRRVVVGLDPRRVDVVLAVLERRLDLTLGGLDVYVNLAGGLKVPDPGLDLPVALAVYSAVVGRALPGNVAVFGEVGLAGE
VRSTQGALRRAEEAARAGYRSLIVPPGLEGREGVRSVEEAVGLVWQPNP

Sequences:

>Translated_449_residues
MAKVTTRYVCTSCGYQSAKPLGRCPNCQAWNSFEEELPAAVPGKTRMGGSGGYGGVVGGKLTPLSTVGRREEPRLPSGIP
ELDRVLGGGLVAGSVTLIGGEPGIGKSTLLLQVADRLARNGQTVLYVAGEESLEQIRLRADRLNVTAEIQLTRDTRAEHI
AALMQEHRPALCIVDSIQTVTVEAEGAPGGVAQVREGTALLTRAAKETGTATVLVGHVTKEGTVAGPKVMEHIVDTTVFL
ETVGAFRLLRSVKNRFGQAGELGVFEMRGEGLVAVENPSAAFLAERPVGVPGSVVAATIDGQRPMLLEVQALAAKTPYPN
PRRVVVGLDPRRVDVVLAVLERRLDLTLGGLDVYVNLAGGLKVPDPGLDLPVALAVYSAVVGRALPGNVAVFGEVGLAGE
VRSTQGALRRAEEAARAGYRSLIVPPGLEGREGVRSVEEAVGLVWQPNP
>Mature_448_residues
AKVTTRYVCTSCGYQSAKPLGRCPNCQAWNSFEEELPAAVPGKTRMGGSGGYGGVVGGKLTPLSTVGRREEPRLPSGIPE
LDRVLGGGLVAGSVTLIGGEPGIGKSTLLLQVADRLARNGQTVLYVAGEESLEQIRLRADRLNVTAEIQLTRDTRAEHIA
ALMQEHRPALCIVDSIQTVTVEAEGAPGGVAQVREGTALLTRAAKETGTATVLVGHVTKEGTVAGPKVMEHIVDTTVFLE
TVGAFRLLRSVKNRFGQAGELGVFEMRGEGLVAVENPSAAFLAERPVGVPGSVVAATIDGQRPMLLEVQALAAKTPYPNP
RRVVVGLDPRRVDVVLAVLERRLDLTLGGLDVYVNLAGGLKVPDPGLDLPVALAVYSAVVGRALPGNVAVFGEVGLAGEV
RSTQGALRRAEEAARAGYRSLIVPPGLEGREGVRSVEEAVGLVWQPNP

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=431, Percent_Identity=47.0997679814385, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47064; Mature: 46933

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVTTRYVCTSCGYQSAKPLGRCPNCQAWNSFEEELPAAVPGKTRMGGSGGYGGVVGGK
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LTPLSTVGRREEPRLPSGIPELDRVLGGGLVAGSVTLIGGEPGIGKSTLLLQVADRLARN
CCCHHHCCCCCCCCCCCCCCHHHHHHCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHCC
GQTVLYVAGEESLEQIRLRADRLNVTAEIQLTRDTRAEHIAALMQEHRPALCIVDSIQTV
CCEEEEEECHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCEEE
TVEAEGAPGGVAQVREGTALLTRAAKETGTATVLVGHVTKEGTVAGPKVMEHIVDTTVFL
EEEECCCCCCHHHHHCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHH
ETVGAFRLLRSVKNRFGQAGELGVFEMRGEGLVAVENPSAAFLAERPVGVPGSVVAATID
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCCCEEEEEEC
GQRPMLLEVQALAAKTPYPNPRRVVVGLDPRRVDVVLAVLERRLDLTLGGLDVYVNLAGG
CCCCEEEEEHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCEEECCEEEEEEECCC
LKVPDPGLDLPVALAVYSAVVGRALPGNVAVFGEVGLAGEVRSTQGALRRAEEAARAGYR
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
SLIVPPGLEGREGVRSVEEAVGLVWQPNP
EEECCCCCCCHHHHHHHHHHHCEEECCCC
>Mature Secondary Structure 
AKVTTRYVCTSCGYQSAKPLGRCPNCQAWNSFEEELPAAVPGKTRMGGSGGYGGVVGGK
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LTPLSTVGRREEPRLPSGIPELDRVLGGGLVAGSVTLIGGEPGIGKSTLLLQVADRLARN
CCCHHHCCCCCCCCCCCCCCHHHHHHCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHCC
GQTVLYVAGEESLEQIRLRADRLNVTAEIQLTRDTRAEHIAALMQEHRPALCIVDSIQTV
CCEEEEEECHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCEEE
TVEAEGAPGGVAQVREGTALLTRAAKETGTATVLVGHVTKEGTVAGPKVMEHIVDTTVFL
EEEECCCCCCHHHHHCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHH
ETVGAFRLLRSVKNRFGQAGELGVFEMRGEGLVAVENPSAAFLAERPVGVPGSVVAATID
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCCCEEEEEEC
GQRPMLLEVQALAAKTPYPNPRRVVVGLDPRRVDVVLAVLERRLDLTLGGLDVYVNLAGG
CCCCEEEEEHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCEEECCEEEEEEECCC
LKVPDPGLDLPVALAVYSAVVGRALPGNVAVFGEVGLAGEVRSTQGALRRAEEAARAGYR
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
SLIVPPGLEGREGVRSVEEAVGLVWQPNP
EEECCCCCCCHHHHHHHHHHHCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]