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The map label for this gene is alaS [H]

Identifier: 94985303

GI number: 94985303

Start: 1276719

End: 1277918

Strand: Reverse

Name: alaS [H]

Synonym: Dgeo_1201

Alternate gene names: 94985303

Gene position: 1277918-1276719 (Counterclockwise)

Preceding gene: 94985304

Following gene: 94985302

Centisome position: 51.8

GC content: 69.33

Gene sequence:

>1200_bases
ATGACCCGGCCGCTCTATGACACGTCGCCCACCCGCCTGACCTTTACGGCTACCGTCACCGACCGGCGTGACGGGGCCGT
CGCGCTGGATGCCAGCGCCTTCTACCCAGAGGGCGGCGGCCAGAACGGTGACGTCGGTGTCTTGCGCTGGCGCGGGGGTG
AAGCGCGCGTTACGGACACCCGCAAGGACAAATCCAGCGGCGTCATTTGGCACGAAGGAGAGGGTGAGCTGCCGCCGGTC
GGCGCTTCCATCCGCGGTGAGGTAGACCCGGTTTGCCGCTGGCGCAACATGGCCCGTCACAGTGCCGAGCATCTGCTCGC
GCAGGCTTTTCACCGCGTCAACCCCGCCTTCGCGGTGGCCGCTGTCAGCCTGCGGAATGCCGAGAGCACGATCGACCTGA
CCGGTGATCCGGGCGAGGCCGATGTGCGCGCCGCGGAGAGGTTGCTGCGGGAGACGCTCGCCCGCACCGAGCTGACGCTG
GAAACGCTGACGGTCCCCGAGGAGGAGCTGTACCGGTACCCGCTGCGCCGCGAAGCGAAGGTGCGGGGCGATGTGCGCCT
GGTGATCTTCCGCGACGCAGAGGGTATCCCCTTCGATGTAAGCGCCTGTGGCGGCACCCATGTTCCGTGTGCCAGCATGG
TCGCTCCGGTTGTCGTGCTGCGCACCGAGCGGATCCGGGGCGGCCTGACCCGCGTGTTCTTCATGGCAGGCGAGGAGGCG
AGTGCGTACCTAGCGGACGTGTACCGTGACGCCCGTGCGTTGGCGCAGAGCTTCAGCGCCTCGGTGTCTGATCTGCCCGG
TCGCGTGGCGGCGCTGGCCACCGAGCGTGACATGCTGAAGGCGGAAGGAACTGCTCTGCGCGCGCGGCTCGCGCGTCTCC
TGGCCGATGCTGCCCCGCTGGAGACGGTCGCAGGTGTTCCGCTGCGTCTCCTGAATCTGGAGGACCCGAACCTCCTCTCC
GACGCGCTGGCCGCCACGCCCAAAGGTGAGGTCCGGGTGGCTCTGGCTTCCGGTGGGCGCTGCGGCGTCGGCAGTGGCCG
GGAGGACGTGTCCGCCGGGAAGCTGTTGGAGGCGGCGCTCAGACTCACGGGTGGCAAGGGGGGCGGGCGGCCCGCGCTGG
CCCAGGGCCGTACCGCTGCGCCCGAACAATTTGGGGAGGCTGTCCGGGAAGTTCTGCAGACTACCCGAGCACCCGCCTAA

Upstream 100 bases:

>100_bases
ACCCCGAGGCGCTGCTCTGCACCTACCCCGGCTGCCGCTGCCAGGAGGTCCGCCGTCTGGTGCGTCAGCAGGGCCTGACG
CCGCGCCCCCACCTGCCTGT

Downstream 100 bases:

>100_bases
GTGCCCGCCCTGCCTCTCAGCCGCCGCACCCGCCAGTCGTAGGGCGCCATCAGAACGGCGGCGGTTACGCGACCCGCCGA
GGAGGGCGGTCATGTCCAGC

Product: threonyl/alanyl tRNA synthetase, SAD

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTRPLYDTSPTRLTFTATVTDRRDGAVALDASAFYPEGGGQNGDVGVLRWRGGEARVTDTRKDKSSGVIWHEGEGELPPV
GASIRGEVDPVCRWRNMARHSAEHLLAQAFHRVNPAFAVAAVSLRNAESTIDLTGDPGEADVRAAERLLRETLARTELTL
ETLTVPEEELYRYPLRREAKVRGDVRLVIFRDAEGIPFDVSACGGTHVPCASMVAPVVVLRTERIRGGLTRVFFMAGEEA
SAYLADVYRDARALAQSFSASVSDLPGRVAALATERDMLKAEGTALRARLARLLADAAPLETVAGVPLRLLNLEDPNLLS
DALAATPKGEVRVALASGGRCGVGSGREDVSAGKLLEAALRLTGGKGGGRPALAQGRTAAPEQFGEAVREVLQTTRAPA

Sequences:

>Translated_399_residues
MTRPLYDTSPTRLTFTATVTDRRDGAVALDASAFYPEGGGQNGDVGVLRWRGGEARVTDTRKDKSSGVIWHEGEGELPPV
GASIRGEVDPVCRWRNMARHSAEHLLAQAFHRVNPAFAVAAVSLRNAESTIDLTGDPGEADVRAAERLLRETLARTELTL
ETLTVPEEELYRYPLRREAKVRGDVRLVIFRDAEGIPFDVSACGGTHVPCASMVAPVVVLRTERIRGGLTRVFFMAGEEA
SAYLADVYRDARALAQSFSASVSDLPGRVAALATERDMLKAEGTALRARLARLLADAAPLETVAGVPLRLLNLEDPNLLS
DALAATPKGEVRVALASGGRCGVGSGREDVSAGKLLEAALRLTGGKGGGRPALAQGRTAAPEQFGEAVREVLQTTRAPA
>Mature_398_residues
TRPLYDTSPTRLTFTATVTDRRDGAVALDASAFYPEGGGQNGDVGVLRWRGGEARVTDTRKDKSSGVIWHEGEGELPPVG
ASIRGEVDPVCRWRNMARHSAEHLLAQAFHRVNPAFAVAAVSLRNAESTIDLTGDPGEADVRAAERLLRETLARTELTLE
TLTVPEEELYRYPLRREAKVRGDVRLVIFRDAEGIPFDVSACGGTHVPCASMVAPVVVLRTERIRGGLTRVFFMAGEEAS
AYLADVYRDARALAQSFSASVSDLPGRVAALATERDMLKAEGTALRARLARLLADAAPLETVAGVPLRLLNLEDPNLLSD
ALAATPKGEVRVALASGGRCGVGSGREDVSAGKLLEAALRLTGGKGGGRPALAQGRTAAPEQFGEAVREVLQTTRAPA

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=382, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR022429
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 42510; Mature: 42378

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPLYDTSPTRLTFTATVTDRRDGAVALDASAFYPEGGGQNGDVGVLRWRGGEARVTDT
CCCCCCCCCCCEEEEEEEEECCCCCCEEEECCEECCCCCCCCCCEEEEEEECCCCEECCC
RKDKSSGVIWHEGEGELPPVGASIRGEVDPVCRWRNMARHSAEHLLAQAFHRVNPAFAVA
CCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AVSLRNAESTIDLTGDPGEADVRAAERLLRETLARTELTLETLTVPEEELYRYPLRREAK
HHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCC
VRGDVRLVIFRDAEGIPFDVSACGGTHVPCASMVAPVVVLRTERIRGGLTRVFFMAGEEA
CCCCEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
SAYLADVYRDARALAQSFSASVSDLPGRVAALATERDMLKAEGTALRARLARLLADAAPL
HHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCH
ETVAGVPLRLLNLEDPNLLSDALAATPKGEVRVALASGGRCGVGSGREDVSAGKLLEAAL
HHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
RLTGGKGGGRPALAQGRTAAPEQFGEAVREVLQTTRAPA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRPLYDTSPTRLTFTATVTDRRDGAVALDASAFYPEGGGQNGDVGVLRWRGGEARVTDT
CCCCCCCCCCEEEEEEEEECCCCCCEEEECCEECCCCCCCCCCEEEEEEECCCCEECCC
RKDKSSGVIWHEGEGELPPVGASIRGEVDPVCRWRNMARHSAEHLLAQAFHRVNPAFAVA
CCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AVSLRNAESTIDLTGDPGEADVRAAERLLRETLARTELTLETLTVPEEELYRYPLRREAK
HHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCC
VRGDVRLVIFRDAEGIPFDVSACGGTHVPCASMVAPVVVLRTERIRGGLTRVFFMAGEEA
CCCCEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
SAYLADVYRDARALAQSFSASVSDLPGRVAALATERDMLKAEGTALRARLARLLADAAPL
HHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCH
ETVAGVPLRLLNLEDPNLLSDALAATPKGEVRVALASGGRCGVGSGREDVSAGKLLEAAL
HHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
RLTGGKGGGRPALAQGRTAAPEQFGEAVREVLQTTRAPA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA