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The map label for this gene is leuC1 [H]

Identifier: 94985258

GI number: 94985258

Start: 1227446

End: 1228705

Strand: Reverse

Name: leuC1 [H]

Synonym: Dgeo_1156

Alternate gene names: 94985258

Gene position: 1228705-1227446 (Counterclockwise)

Preceding gene: 94985261

Following gene: 94985257

Centisome position: 49.8

GC content: 65.71

Gene sequence:

>1260_bases
ATGAGTGCTTCTTCCCCCCGCCCGCAGACGATGGCGGAAAAGATCCTCTCGCGGCGCGGCCACCAGACCGTGTATGCCGG
GGACCTTGCCGTCGTGGACGTGGATCAGGTGATGGTCGTGGACTCCATCGCGCAGAGCTTTATCGCGCGGATGGAAGAGG
ACCTTGCCGCCACCCCGAAATACCCCGGGCGTGTTTCTATCGTCATCGACCACGTCGCGCCCGCCTCTACCGTGAGCGTC
GCGCAGGCACAGAAGGAGGCCCGCGAGTACGCCGCGAAGACGGGCGTCCGCCTGTTCGACGTCGGAAGGGGCATCTGCCA
CCAGGTCCTGATGGAGGAGCGCCTCGCACAACCCGGCTGGATTGTGTTGGGGTCGGACAGCCATTCGACCACCTACGGCG
CCGTCGCCGCCTTTGGCACTGGCATGGGTGCCACCGACATCGCCCTCGCTGCCGCCAGCGGCAAGACTTGGCTGCGGGTG
CCGGAAAGCGTGAAGGTGACCTTTGTGGGCGAACTCCAGCCCGGTGTGACCGCCAAGGACGCCGCCCTGGAAATGATTCG
CCTCTTGGGGGCGGACGGGGCCACCTACCAGAGCATCGAGATGCACCCCGGAGACCGCTTCACGCGCGGCGAGCGGATGA
CGCTGGCAAACCTCTGCGTGGAGGCGGGCGCGAAGGCAGGCCTGGTCGTTCCCGGCGGCGAAATCCTGACCGTCTACGGC
TACGATATCCCGGAGTGGGTGTACCCCGACTCCGGTGCTGAATATATACAAGAGATCGAGATTGATCTCACGGCCCTCCA
CCCCCGCATGAGTGCCCCCAGCGAGGTGGACAATGTCCATGACGTGGCGGAGCTGCGCGGCCTGAAGGTGGATCAGGTGT
TTATTGGCACCTGCACAAATGGCCGCTTGGAAGACCTGCATGCCGCCGCCGAGGTGCTGAGGGGTCAGCGGGTCGATCCC
TCCACTCGTCTTCTGGTCATTCCGGCCAGCAGCGAGGTGATGGCGGCGGCCTTGAGTGACGGAACCCTGCTCACGCTGAT
GCAGGCGGGTGCCGTGCTGGGGACGCCCGGCTGCGGTCCCTGTATGGGCCGTCACCAGGGCGTGCTTGCCGCGGGCGAGG
TCTGCGTCTCCACAAGTAACCGCAATTTCATCGGGCGCATGGGCGACAAGGACGCCAAGATCTACCTCGCTTCGCCCGCA
GTGGCGGCTGCGACGGCGGTGATGGGGCGGATTGCCCTGCCGGAAGACCTGAAGGCGTGA

Upstream 100 bases:

>100_bases
AGCGCCGGTTGAATCGTGTGACCTGGCAAATGACAGACCGGAAGGCGCTTTGAGCATTCAACCTCATCCCTCATCCGGAC
TTTCGCGCGAGAATAGCCCC

Downstream 100 bases:

>100_bases
ATGCCCCAGCCCACTTTCCTCCGTTCCATCTGCACTGTTCCTGTGACACCGGGCACCCACCTCGAAGACCAGCGGCTATG
GACACGTGTGTTTGCTGGCT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSASSPRPQTMAEKILSRRGHQTVYAGDLAVVDVDQVMVVDSIAQSFIARMEEDLAATPKYPGRVSIVIDHVAPASTVSV
AQAQKEAREYAAKTGVRLFDVGRGICHQVLMEERLAQPGWIVLGSDSHSTTYGAVAAFGTGMGATDIALAAASGKTWLRV
PESVKVTFVGELQPGVTAKDAALEMIRLLGADGATYQSIEMHPGDRFTRGERMTLANLCVEAGAKAGLVVPGGEILTVYG
YDIPEWVYPDSGAEYIQEIEIDLTALHPRMSAPSEVDNVHDVAELRGLKVDQVFIGTCTNGRLEDLHAAAEVLRGQRVDP
STRLLVIPASSEVMAAALSDGTLLTLMQAGAVLGTPGCGPCMGRHQGVLAAGEVCVSTSNRNFIGRMGDKDAKIYLASPA
VAAATAVMGRIALPEDLKA

Sequences:

>Translated_419_residues
MSASSPRPQTMAEKILSRRGHQTVYAGDLAVVDVDQVMVVDSIAQSFIARMEEDLAATPKYPGRVSIVIDHVAPASTVSV
AQAQKEAREYAAKTGVRLFDVGRGICHQVLMEERLAQPGWIVLGSDSHSTTYGAVAAFGTGMGATDIALAAASGKTWLRV
PESVKVTFVGELQPGVTAKDAALEMIRLLGADGATYQSIEMHPGDRFTRGERMTLANLCVEAGAKAGLVVPGGEILTVYG
YDIPEWVYPDSGAEYIQEIEIDLTALHPRMSAPSEVDNVHDVAELRGLKVDQVFIGTCTNGRLEDLHAAAEVLRGQRVDP
STRLLVIPASSEVMAAALSDGTLLTLMQAGAVLGTPGCGPCMGRHQGVLAAGEVCVSTSNRNFIGRMGDKDAKIYLASPA
VAAATAVMGRIALPEDLKA
>Mature_418_residues
SASSPRPQTMAEKILSRRGHQTVYAGDLAVVDVDQVMVVDSIAQSFIARMEEDLAATPKYPGRVSIVIDHVAPASTVSVA
QAQKEAREYAAKTGVRLFDVGRGICHQVLMEERLAQPGWIVLGSDSHSTTYGAVAAFGTGMGATDIALAAASGKTWLRVP
ESVKVTFVGELQPGVTAKDAALEMIRLLGADGATYQSIEMHPGDRFTRGERMTLANLCVEAGAKAGLVVPGGEILTVYGY
DIPEWVYPDSGAEYIQEIEIDLTALHPRMSAPSEVDNVHDVAELRGLKVDQVFIGTCTNGRLEDLHAAAEVLRGQRVDPS
TRLLVIPASSEVMAAALSDGTLLTLMQAGAVLGTPGCGPCMGRHQGVLAAGEVCVSTSNRNFIGRMGDKDAKIYLASPAV
AAATAVMGRIALPEDLKA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=354, Percent_Identity=29.6610169491525, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1786259, Length=416, Percent_Identity=31.4903846153846, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081781, Length=346, Percent_Identity=27.4566473988439, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367097, Length=161, Percent_Identity=34.7826086956522, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=452, Percent_Identity=29.2035398230088, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=350, Percent_Identity=29.7142857142857, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.2253521126761, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320440, Length=453, Percent_Identity=32.4503311258278, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321429, Length=376, Percent_Identity=31.1170212765957, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323335, Length=458, Percent_Identity=26.4192139737991, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=464, Percent_Identity=25.2155172413793, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571643, Length=350, Percent_Identity=27.1428571428571, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281365315, Length=450, Percent_Identity=25.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17864292, Length=450, Percent_Identity=25.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161076999, Length=372, Percent_Identity=26.0752688172043, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44197; Mature: 44066

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASSPRPQTMAEKILSRRGHQTVYAGDLAVVDVDQVMVVDSIAQSFIARMEEDLAATPK
CCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
YPGRVSIVIDHVAPASTVSVAQAQKEAREYAAKTGVRLFDVGRGICHQVLMEERLAQPGW
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCE
IVLGSDSHSTTYGAVAAFGTGMGATDIALAAASGKTWLRVPESVKVTFVGELQPGVTAKD
EEEECCCCCCHHHHHHHHCCCCCCHHHEEEECCCCEEEECCCCEEEEEEECCCCCCCHHH
AALEMIRLLGADGATYQSIEMHPGDRFTRGERMTLANLCVEAGAKAGLVVPGGEILTVYG
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCEEEEEC
YDIPEWVYPDSGAEYIQEIEIDLTALHPRMSAPSEVDNVHDVAELRGLKVDQVFIGTCTN
CCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCC
GRLEDLHAAAEVLRGQRVDPSTRLLVIPASSEVMAAALSDGTLLTLMQAGAVLGTPGCGP
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHCCCCEEEHHHCCCEECCCCCCC
CMGRHQGVLAAGEVCVSTSNRNFIGRMGDKDAKIYLASPAVAAATAVMGRIALPEDLKA
CCCCCCCCEEECCEEEECCCCCEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
SASSPRPQTMAEKILSRRGHQTVYAGDLAVVDVDQVMVVDSIAQSFIARMEEDLAATPK
CCCCCCCHHHHHHHHHHCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
YPGRVSIVIDHVAPASTVSVAQAQKEAREYAAKTGVRLFDVGRGICHQVLMEERLAQPGW
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCE
IVLGSDSHSTTYGAVAAFGTGMGATDIALAAASGKTWLRVPESVKVTFVGELQPGVTAKD
EEEECCCCCCHHHHHHHHCCCCCCHHHEEEECCCCEEEECCCCEEEEEEECCCCCCCHHH
AALEMIRLLGADGATYQSIEMHPGDRFTRGERMTLANLCVEAGAKAGLVVPGGEILTVYG
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCEEEEEC
YDIPEWVYPDSGAEYIQEIEIDLTALHPRMSAPSEVDNVHDVAELRGLKVDQVFIGTCTN
CCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCC
GRLEDLHAAAEVLRGQRVDPSTRLLVIPASSEVMAAALSDGTLLTLMQAGAVLGTPGCGP
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHCCCCEEEHHHCCCEECCCCCCC
CMGRHQGVLAAGEVCVSTSNRNFIGRMGDKDAKIYLASPAVAAATAVMGRIALPEDLKA
CCCCCCCCEEECCEEEECCCCCEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]