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The map label for this gene is lysX [H]

Identifier: 94985253

GI number: 94985253

Start: 1224678

End: 1225556

Strand: Reverse

Name: lysX [H]

Synonym: Dgeo_1151

Alternate gene names: 94985253

Gene position: 1225556-1224678 (Counterclockwise)

Preceding gene: 94985254

Following gene: 94985250

Centisome position: 49.67

GC content: 66.33

Gene sequence:

>879_bases
ATGGCCGAACTTGCCGTTCTCTACGACCGTATTCGCCCCGACGAGAAGATGCTCTTCGGGGCGCTCGATGCCCTCGGTGT
GCCCTATGACAAGGTCTACACGCCGCAACTGCACGTCACCTTTGATGAGGCGGGGCGCGCGCGGGTGCCCTGGCGGGTTG
CGCTCGAACGCTGCGTCAGTCAAACGCGAGGGCACGCCGTCACGCGGGCCCTGGAGGGGTTGGGCGTACGCGTCGTGAAT
CCCTCGCACGTTATCGAGCTGTGCGGCGACAAGCTCGCCACCAACGCTGCCCTTGCCCGCGCGGGGTTGCCCACGCCGCG
CACCGGTGTCGCCTTTGACGGCGAGACTGCTCTGGCTCTGATCGAGGAGCTGGGCTACCCAGTCGTCCTCAAGCCGACCG
TGGGTTCCTGGGGGCGGATGGTCAGCCGCATCAACGACCGTGACGCCGCCGAGGCGCTGATCGAGCACAAGGAGGTGCTC
GGCGGGCCGCAGCATGGCGTTTTCTACGTGCAGGAGCTGATTCACAAACCGGGACGCGACATCCGCGCTTTTGTGGTGGG
CGGGGAGTGCATCGGGGCGATCTACCGCACGAGCGAGCACTGGATCACGAACACCGCGCGGGGTGCCAAGGCCAGCAACT
GCCCGGTGACGCCCGAGATCGCGTCCCTGGCGACCCGCGCCGCCGCTGCGGTTGGAGGCGAGATCGTCGCCATCGACCTG
GTCGAGGACCCGCAGCGCCGAAACGAGTGGGGCGGCCTGCTGGTCATTGAGGTCAATCACACCATGGAGTTCAAAAACTC
GGTGAGCACGACTGGCGTTGACATTCCCCGTCAGATGGGTGAGTACGCTCTGGGCCTGCTGGCTGGCGTGCTTGCCTGA

Upstream 100 bases:

>100_bases
CAGGCCCCGCAGGAAGCGGAGGACTGGGGAGAGTAAGGCGTGAGGGCTTAGGGGTGAGGAACAGCCTCCCCCTGACTCTT
GACGCCTTCCCCCTTTCCTT

Downstream 100 bases:

>100_bases
CTCCAGCTCCACCCTGACTCCAGCTTGACCAGGGCGGGCGGTTCTCCTCTGATGGGGAGGCCGCCGCCTGGCTGTCTCAG
CGGTACAAGGCCAGGTTCAC

Product: lysine biosynthesis enzyme LysX

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAELAVLYDRIRPDEKMLFGALDALGVPYDKVYTPQLHVTFDEAGRARVPWRVALERCVSQTRGHAVTRALEGLGVRVVN
PSHVIELCGDKLATNAALARAGLPTPRTGVAFDGETALALIEELGYPVVLKPTVGSWGRMVSRINDRDAAEALIEHKEVL
GGPQHGVFYVQELIHKPGRDIRAFVVGGECIGAIYRTSEHWITNTARGAKASNCPVTPEIASLATRAAAAVGGEIVAIDL
VEDPQRRNEWGGLLVIEVNHTMEFKNSVSTTGVDIPRQMGEYALGLLAGVLA

Sequences:

>Translated_292_residues
MAELAVLYDRIRPDEKMLFGALDALGVPYDKVYTPQLHVTFDEAGRARVPWRVALERCVSQTRGHAVTRALEGLGVRVVN
PSHVIELCGDKLATNAALARAGLPTPRTGVAFDGETALALIEELGYPVVLKPTVGSWGRMVSRINDRDAAEALIEHKEVL
GGPQHGVFYVQELIHKPGRDIRAFVVGGECIGAIYRTSEHWITNTARGAKASNCPVTPEIASLATRAAAAVGGEIVAIDL
VEDPQRRNEWGGLLVIEVNHTMEFKNSVSTTGVDIPRQMGEYALGLLAGVLA
>Mature_291_residues
AELAVLYDRIRPDEKMLFGALDALGVPYDKVYTPQLHVTFDEAGRARVPWRVALERCVSQTRGHAVTRALEGLGVRVVNP
SHVIELCGDKLATNAALARAGLPTPRTGVAFDGETALALIEELGYPVVLKPTVGSWGRMVSRINDRDAAEALIEHKEVLG
GPQHGVFYVQELIHKPGRDIRAFVVGGECIGAIYRTSEHWITNTARGAKASNCPVTPEIASLATRAAAAVGGEIVAIDLV
EDPQRRNEWGGLLVIEVNHTMEFKNSVSTTGVDIPRQMGEYALGLLAGVLA

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=230, Percent_Identity=31.7391304347826, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787076, Length=220, Percent_Identity=34.0909090909091, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31474; Mature: 31343

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELAVLYDRIRPDEKMLFGALDALGVPYDKVYTPQLHVTFDEAGRARVPWRVALERCVS
CCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEECCEEEEEECCCCCCCCCHHHHHHHHHH
QTRGHAVTRALEGLGVRVVNPSHVIELCGDKLATNAALARAGLPTPRTGVAFDGETALAL
HHCCHHHHHHHHHCCEEEECHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCHHHHHH
IEELGYPVVLKPTVGSWGRMVSRINDRDAAEALIEHKEVLGGPQHGVFYVQELIHKPGRD
HHHCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
IRAFVVGGECIGAIYRTSEHWITNTARGAKASNCPVTPEIASLATRAAAAVGGEIVAIDL
EEEEEECCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
VEDPQRRNEWGGLLVIEVNHTMEFKNSVSTTGVDIPRQMGEYALGLLAGVLA
CCCCHHHCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AELAVLYDRIRPDEKMLFGALDALGVPYDKVYTPQLHVTFDEAGRARVPWRVALERCVS
CHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEECCEEEEEECCCCCCCCCHHHHHHHHHH
QTRGHAVTRALEGLGVRVVNPSHVIELCGDKLATNAALARAGLPTPRTGVAFDGETALAL
HHCCHHHHHHHHHCCEEEECHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCHHHHHH
IEELGYPVVLKPTVGSWGRMVSRINDRDAAEALIEHKEVLGGPQHGVFYVQELIHKPGRD
HHHCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
IRAFVVGGECIGAIYRTSEHWITNTARGAKASNCPVTPEIASLATRAAAAVGGEIVAIDL
EEEEEECCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
VEDPQRRNEWGGLLVIEVNHTMEFKNSVSTTGVDIPRQMGEYALGLLAGVLA
CCCCHHHCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379 [H]